Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ABHN08_RS19465 Genome accession   NZ_CP157354
Coordinates   4296606..4297823 (+) Length   405 a.a.
NCBI ID   WP_024014342.1    Uniprot ID   V8R166
Organism   Pseudomonas atacamensis strain GY-AP11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4291606..4302823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHN08_RS19445 (ABHN08_19445) - 4293048..4293347 (+) 300 WP_348970149.1 DUF2845 domain-containing protein -
  ABHN08_RS19450 (ABHN08_19450) - 4293612..4293965 (-) 354 WP_016773155.1 BON domain-containing protein -
  ABHN08_RS19455 (ABHN08_19455) - 4294260..4294676 (-) 417 WP_056790436.1 pilin -
  ABHN08_RS19460 (ABHN08_19460) pilB 4294903..4296603 (+) 1701 WP_073475466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABHN08_RS19465 (ABHN08_19465) pilC 4296606..4297823 (+) 1218 WP_024014342.1 type II secretion system F family protein Machinery gene
  ABHN08_RS19470 (ABHN08_19470) pilD 4297825..4298697 (+) 873 WP_122747336.1 prepilin peptidase Machinery gene
  ABHN08_RS19475 (ABHN08_19475) coaE 4298694..4299317 (+) 624 WP_186569367.1 dephospho-CoA kinase -
  ABHN08_RS19480 (ABHN08_19480) yacG 4299314..4299514 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  ABHN08_RS19485 (ABHN08_19485) - 4299522..4299737 (-) 216 WP_016773148.1 hypothetical protein -
  ABHN08_RS19490 (ABHN08_19490) - 4299803..4300492 (-) 690 WP_016773147.1 energy-coupling factor ABC transporter permease -
  ABHN08_RS19495 (ABHN08_19495) - 4300610..4301236 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  ABHN08_RS19500 (ABHN08_19500) - 4301233..4301760 (+) 528 WP_024014346.1 MOSC domain-containing protein -
  ABHN08_RS19505 (ABHN08_19505) - 4301812..4301985 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43995.68 Da        Isoelectric Point: 10.1214

>NTDB_id=1006213 ABHN08_RS19465 WP_024014342.1 4296606..4297823(+) (pilC) [Pseudomonas atacamensis strain GY-AP11]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSLGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1006213 ABHN08_RS19465 WP_024014342.1 4296606..4297823(+) (pilC) [Pseudomonas atacamensis strain GY-AP11]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGGCAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAAGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CCTCCCTGCTGAGCTTGGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCAACGATGATGAAA
GCCGGTGTACCGCTGTTGCAGTCCTTCGACATCATCGGCGAGGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGATGA
GGTCAAACAGGAAGTTGCCGCCGGTAACAGTTTCGCCACGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGACGCCGGCGAACAGTCCGGTGCCCTCGACACCTTGCTCGAACGAGTGGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCGGTGGTGACCGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTTCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCGATTCTGTTTGCACTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACATCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTTGCACGCTTTGCCCGCACCCTGTCGACCACTTTCGCCGCCGGTGTGCCGCTGGTCGAAGCACTGG
ATTCGGTGGCGGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCTACGGGTTCGGCAGGACGTCTCGACCGGCATG
CAGCTCAACTTCTCGATGCGCAGCACCGGTGTGTTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAGTCCGG
GGCACTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGTCTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8R166

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.949

97.778

0.44
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

39.85

98.519

0.393

Multiple sequence alignment