Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   QUD59_RS03735 Genome accession   NZ_AP027292
Coordinates   804433..805653 (+) Length   406 a.a.
NCBI ID   WP_286239683.1    Uniprot ID   -
Organism   Neptuniibacter halophilus strain LMG 25378     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 799433..810653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD59_RS03710 ampD 800009..800557 (-) 549 WP_286239676.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUD59_RS03715 nadC 800673..801521 (+) 849 WP_286239677.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUD59_RS03720 - 801607..801948 (-) 342 WP_286239679.1 hypothetical protein -
  QUD59_RS03725 - 802057..802500 (-) 444 WP_286239680.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QUD59_RS03730 pilB 802704..804416 (+) 1713 WP_286239682.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUD59_RS03735 pilC 804433..805653 (+) 1221 WP_286239683.1 type II secretion system F family protein Machinery gene
  QUD59_RS03740 pilD 805669..806517 (+) 849 WP_286239685.1 A24 family peptidase Machinery gene
  QUD59_RS03745 coaE 806520..807125 (+) 606 WP_350227791.1 dephospho-CoA kinase -
  QUD59_RS03750 yacG 807122..807349 (+) 228 WP_286239686.1 DNA gyrase inhibitor YacG -
  QUD59_RS03755 - 807350..808294 (-) 945 WP_286239687.1 Nudix family hydrolase -
  QUD59_RS03760 argJ 808294..809514 (-) 1221 WP_286239688.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44113.60 Da        Isoelectric Point: 9.3090

>NTDB_id=100611 QUD59_RS03735 WP_286239683.1 804433..805653(+) (pilC) [Neptuniibacter halophilus strain LMG 25378]
MPKEAKLQTFSWQGKDKNGNKSKGKLDAESLSVAKAQLRKQGIISPKVSKEIEIGFLSSKNKPIKPVDIAFFTRQMATML
KSGVPLMQGLDITAGGMDKQKLKEMVYAIKNDVNSGLDFSTSLTKYPEHFDDLYCNLVHAGEQSGALEQMLDRIATYKEK
LETLKGKIKKALTYPTAVIVVGLIVSAILLVKVVPQFEMVFKNFGADLPAFTMFVVGLSEVAQEWWWVALLSIAAAGVSF
SKARARSQKFSDSVDRFMLKIPVIGKILQDASVARFSRTLATTFAAGVPLVNALESAAGASGNALYRDAILQIRNGVSTG
QSLQNAINMTGIFPNMAVQMISIGEEAGSLEMMLEKVADFYEEQVDNAVDNLSSLLEPLIMVVLGVLVGGLVVAMYLPIF
QLGNVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=100611 QUD59_RS03735 WP_286239683.1 804433..805653(+) (pilC) [Neptuniibacter halophilus strain LMG 25378]
ATGCCGAAAGAAGCCAAACTGCAGACGTTTTCCTGGCAGGGCAAGGATAAAAATGGCAACAAAAGTAAAGGTAAGCTTGA
CGCTGAAAGCCTCTCTGTTGCCAAAGCACAATTGCGCAAACAGGGCATTATTTCTCCCAAAGTCAGCAAAGAGATTGAGA
TTGGCTTTTTAAGCAGCAAAAATAAGCCGATTAAACCTGTGGATATCGCTTTTTTCACCCGACAGATGGCGACCATGCTC
AAATCCGGTGTACCGCTGATGCAAGGACTGGATATCACTGCAGGCGGCATGGATAAACAAAAGCTCAAAGAGATGGTTTA
TGCCATTAAAAATGATGTAAACAGCGGCCTCGACTTTTCGACATCCCTGACCAAGTATCCCGAACATTTTGATGATCTCT
ACTGTAATCTGGTTCATGCAGGCGAGCAGTCGGGTGCTCTTGAGCAGATGCTCGATCGAATTGCGACCTATAAAGAGAAG
CTGGAGACTCTCAAAGGTAAAATCAAAAAAGCATTGACCTACCCTACCGCTGTTATTGTGGTGGGCTTGATTGTGTCGGC
TATATTGCTGGTCAAAGTGGTCCCACAGTTCGAGATGGTATTCAAGAACTTCGGTGCCGACCTGCCCGCATTTACGATGT
TTGTTGTCGGTCTGTCTGAAGTCGCACAGGAGTGGTGGTGGGTAGCCCTGCTTAGTATTGCAGCAGCCGGCGTTTCTTTC
AGTAAAGCCAGAGCCCGATCCCAGAAATTCAGTGACTCGGTTGATCGTTTCATGCTGAAAATACCAGTAATCGGCAAAAT
TCTGCAAGACGCTTCCGTGGCTCGATTTTCGCGTACACTGGCCACTACCTTTGCCGCAGGTGTGCCTTTGGTCAATGCTC
TTGAGTCAGCCGCGGGCGCCTCTGGTAACGCCCTCTATCGGGATGCGATTCTGCAGATCCGGAATGGGGTATCCACCGGT
CAGTCATTACAAAATGCCATCAATATGACCGGCATTTTCCCCAACATGGCTGTACAGATGATCTCCATTGGTGAGGAAGC
CGGCTCTCTGGAGATGATGCTGGAAAAAGTCGCCGACTTCTATGAAGAGCAGGTGGATAACGCGGTTGATAACCTCTCCA
GCCTGCTGGAACCGCTGATCATGGTCGTACTGGGCGTACTGGTTGGCGGTCTGGTAGTTGCTATGTACCTGCCTATATTC
CAGTTGGGTAACGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.168

99.507

0.579
  pilC Legionella pneumophila strain ERS1305867

56

98.522

0.552
  pilC Acinetobacter baumannii D1279779

53.25

98.522

0.525
  pilC Acinetobacter baylyi ADP1

52.75

98.522

0.52
  pilC Vibrio cholerae strain A1552

42.289

99.015

0.419
  pilG Neisseria gonorrhoeae MS11

42.289

99.015

0.419
  pilG Neisseria meningitidis 44/76-A

42.04

99.015

0.416
  pilC Vibrio campbellii strain DS40M4

41.895

98.768

0.414

Multiple sequence alignment