Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUD59_RS03730 Genome accession   NZ_AP027292
Coordinates   802704..804416 (+) Length   570 a.a.
NCBI ID   WP_286239682.1    Uniprot ID   -
Organism   Neptuniibacter halophilus strain LMG 25378     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797704..809416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD59_RS03700 - 797722..799119 (-) 1398 WP_286239674.1 DUF4856 domain-containing protein -
  QUD59_RS03705 - 799298..800008 (+) 711 WP_286239675.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUD59_RS03710 ampD 800009..800557 (-) 549 WP_286239676.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUD59_RS03715 nadC 800673..801521 (+) 849 WP_286239677.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUD59_RS03720 - 801607..801948 (-) 342 WP_286239679.1 hypothetical protein -
  QUD59_RS03725 - 802057..802500 (-) 444 WP_286239680.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QUD59_RS03730 pilB 802704..804416 (+) 1713 WP_286239682.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUD59_RS03735 pilC 804433..805653 (+) 1221 WP_286239683.1 type II secretion system F family protein Machinery gene
  QUD59_RS03740 pilD 805669..806517 (+) 849 WP_286239685.1 A24 family peptidase Machinery gene
  QUD59_RS03745 coaE 806520..807125 (+) 606 WP_350227791.1 dephospho-CoA kinase -
  QUD59_RS03750 yacG 807122..807349 (+) 228 WP_286239686.1 DNA gyrase inhibitor YacG -
  QUD59_RS03755 - 807350..808294 (-) 945 WP_286239687.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63170.03 Da        Isoelectric Point: 4.8893

>NTDB_id=100610 QUD59_RS03730 WP_286239682.1 802704..804416(+) (pilB) [Neptuniibacter halophilus strain LMG 25378]
MQSRQPLSGLAKRLVNEGVCSAEVAADAAQQCRDKQQTFISYVIEQRLVSAYRAAAAASEEFGIPLLDLDALDMSSLPQG
LINETLITKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAVIVEEDKLTRAIESFLDNQGSALDDLEDSDLD
NLEIDDATPGEQEDDSGEDAANDAPIVRFVNKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLASRLSA
RLKVMSQMDISERRLPQDGRIKMKISKNRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIEALGFEPFQKDLYMTTLHKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVHAKVGLTFAEALRAFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCEGCKKPLQLPETTLREEGF
SEEQIKELNLFEANKEGCNKCNRGYKGRVGIYEMLPMSQEVAEVIMENGTSLDILKVAEQQNFQTLRRSGLKKVAEGVTS
LEEMNRVIKI

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=100610 QUD59_RS03730 WP_286239682.1 802704..804416(+) (pilB) [Neptuniibacter halophilus strain LMG 25378]
TTGCAGTCACGTCAACCACTCAGCGGCCTCGCTAAAAGGCTCGTCAACGAAGGGGTCTGTTCTGCCGAAGTCGCAGCGGA
CGCTGCCCAGCAGTGCCGCGATAAACAGCAGACCTTTATCTCCTATGTGATTGAGCAGCGTCTGGTCAGCGCCTACCGCG
CAGCCGCAGCAGCGTCGGAGGAGTTCGGCATCCCTCTGCTGGATCTGGATGCACTGGATATGAGCAGCCTCCCTCAGGGG
CTGATCAATGAAACCCTGATTACCAAACACCATGCGCTGCCCTTGCAAAAGCGCGATAACCGACTGTTTGTGGCCATTTC
TGACCCGACAAACATTCAGGCGCTGGATGAATTCAAGTTTCAGAGCGGGATCACCACCGAGGCAGTGATTGTCGAAGAAG
ACAAACTCACCCGGGCGATTGAATCCTTTCTGGATAATCAGGGCTCTGCTCTGGACGACCTGGAAGACAGCGATCTTGAT
AATCTGGAGATCGATGATGCCACTCCCGGTGAGCAGGAAGATGATAGCGGTGAAGATGCCGCCAACGATGCGCCGATCGT
CCGCTTCGTTAACAAGATACTTCTCGACTCGATTAAAAACGGCGCCTCGGATATCCACTTCGAGCCCTATGAGAAAAGTT
ACCGTATCCGTAGTCGTATCGATGGCATCCTGCAGGAGGTTGCCAAACCTCCTTCTAATCTGGCGTCGCGTCTTTCGGCA
CGCCTGAAGGTTATGTCACAGATGGATATATCAGAACGACGCCTCCCGCAGGATGGTCGTATAAAGATGAAAATATCCAA
AAATCGCGCCATCGATTTCCGTGTTAACACCCTCCCCACCCTGTGGGGGGAGAAGATCGTGCTGCGTATCCTCGACCCCA
GCAGCGCTAAGATGGGCATTGAGGCGCTGGGCTTTGAGCCTTTCCAGAAAGATCTGTATATGACGACCCTGCACAAGCCA
CAGGGAATGATTCTGGTAACCGGCCCTACCGGTAGCGGTAAAACCGTGAGTCTGTATACCGGCCTGAACATACTGAATAC
CGAGGAGCGAAACATCTCGACCGCAGAAGACCCGGTAGAGATAAACCTCGAGGGGATCAATCAGGTTCACGTGCACGCCA
AAGTCGGCCTTACCTTTGCTGAAGCCCTGCGTGCCTTCTTGCGACAGGATCCTGACGTAGTCATGGTGGGTGAGATCCGG
GATCTGGAAACCGCCGAAATTGCGATCAAAGCGGCCCAGACCGGGCACATGGTACTCTCCACCCTGCACACCAACAGTGC
ACCGGAAACCCTGACCCGACTGCGCAACATGGGTGTACCGGCCTTCAATATCGCCACCTCAGTCAGCCTGATTATTGCCC
AGCGTCTGGGGCGCCGTCTCTGCGAAGGTTGCAAAAAGCCCCTTCAGTTGCCCGAAACCACACTGCGTGAAGAGGGATTC
TCGGAAGAGCAGATCAAAGAACTGAATCTGTTCGAAGCCAACAAAGAGGGCTGCAACAAGTGCAACCGAGGCTACAAAGG
CCGGGTAGGTATCTACGAGATGCTGCCGATGAGCCAGGAAGTGGCTGAAGTAATCATGGAGAACGGTACCTCTTTGGATA
TACTTAAGGTGGCCGAACAACAAAATTTCCAGACATTAAGACGTTCCGGGCTGAAAAAAGTAGCCGAAGGGGTTACCAGT
CTGGAAGAGATGAACCGCGTAATCAAAATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.596

100

0.586
  pilB Acinetobacter baylyi ADP1

58.792

98.772

0.581
  pilB Legionella pneumophila strain ERS1305867

54.947

99.298

0.546
  pilB Vibrio cholerae strain A1552

55.491

91.053

0.505
  pilB Vibrio campbellii strain DS40M4

54.264

90.526

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

54.102

89.825

0.486
  pilF Neisseria gonorrhoeae MS11

48.33

99.825

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

40.234

89.825

0.361

Multiple sequence alignment