Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   E0F40_RS10320 Genome accession   NZ_LR216065
Coordinates   1903991..1904932 (-) Length   313 a.a.
NCBI ID   WP_000249550.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1898991..1909932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F40_RS12365 (SAMEA3714487_01961) - 1899003..1899125 (-) 123 WP_000343850.1 hypothetical protein -
  E0F40_RS10290 (SAMEA3714487_01962) - 1899199..1899474 (-) 276 WP_001094372.1 hypothetical protein -
  E0F40_RS10295 (SAMEA3714487_01963) irvR 1899649..1900455 (+) 807 WP_000090700.1 XRE family transcriptional regulator Regulator
  E0F40_RS10300 (SAMEA3714487_01964) - 1900457..1901221 (+) 765 WP_000032361.1 type II toxin-antitoxin system PemK/MazF family toxin -
  E0F40_RS10305 (SAMEA3714487_01965) - 1901500..1902945 (+) 1446 WP_050105991.1 recombinase family protein -
  E0F40_RS10315 (SAMEA3714487_01966) comGB/cglB 1903027..1904043 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F40_RS10320 (SAMEA3714487_01967) comGA/cglA/cilD 1903991..1904932 (-) 942 WP_000249550.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F40_RS10325 (SAMEA3714487_01968) - 1905008..1905373 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F40_RS10330 (SAMEA3714487_01969) - 1905524..1906582 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F40_RS10335 (SAMEA3714487_01970) nagA 1906745..1907896 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  E0F40_RS10340 (SAMEA3714487_01971) - 1908049..1909866 (-) 1818 WP_001220850.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35516.43 Da        Isoelectric Point: 6.0083

>NTDB_id=1004144 E0F40_RS10320 WP_000249550.1 1903991..1904932(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1004144 E0F40_RS10320 WP_000249550.1 1903991..1904932(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAAAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comGA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comGA Streptococcus mutans UA159

66.238

99.361

0.658
  comGA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364