Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AACH30_RS00660 Genome accession   NZ_AP027243
Coordinates   136723..137667 (+) Length   314 a.a.
NCBI ID   WP_265150863.1    Uniprot ID   A0AA43TCR2
Organism   Lactococcus garvieae strain L21-68     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131723..142667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH30_RS00645 (LG21E68_01210) - 133133..134587 (-) 1455 WP_265149966.1 GH25 family lysozyme -
  AACH30_RS00650 (LG21E68_01220) guaB 134756..136237 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  AACH30_RS00655 (LG21E68_01230) rpsU 136360..136536 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  AACH30_RS00660 (LG21E68_01240) comGA 136723..137667 (+) 945 WP_265150863.1 competence type IV pilus ATPase ComGA Machinery gene
  AACH30_RS00665 (LG21E68_01250) comGB 137606..138625 (+) 1020 WP_016171027.1 competence type IV pilus assembly protein ComGB Machinery gene
  AACH30_RS00670 (LG21E68_01260) comYC 138644..138949 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  AACH30_RS00675 comGD 138924..139340 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  AACH30_RS00680 comGE 139372..139605 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  AACH30_RS00685 (LG21E68_01280) comGF 139586..140026 (+) 441 WP_338129529.1 competence type IV pilus minor pilin ComGF -
  AACH30_RS00690 (LG21E68_01290) - 140259..141104 (+) 846 WP_023889974.1 zinc ABC transporter substrate-binding protein -
  AACH30_RS00695 (LG21E68_01300) - 141190..142059 (-) 870 WP_023889973.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35852.07 Da        Isoelectric Point: 6.6641

>NTDB_id=100286 AACH30_RS00660 WP_265150863.1 136723..137667(+) (comGA) [Lactococcus garvieae strain L21-68]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVISR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=100286 AACH30_RS00660 WP_265150863.1 136723..137667(+) (comGA) [Lactococcus garvieae strain L21-68]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGGCCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG
CTGTTCTTCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCGCGCTCAATATCAGGTGTAATTTCACGG
CTAAAGGAACTTGGATTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGATGAATGGAACGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.554

100

0.596
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.077

99.682

0.519
  comYA Streptococcus mutans UA159

51.282

99.363

0.51
  comYA Streptococcus mutans UA140

51.282

99.363

0.51

Multiple sequence alignment