Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   ECRM13516_RS17485 Genome accession   NZ_CP006262
Coordinates   3414758..3415750 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O145:H28 str. RM13516     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 3409758..3420750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECRM13516_RS17460 (ECRM13516_3453) pphB 3411121..3411777 (+) 657 WP_025380568.1 protein-serine/threonine phosphatase -
  ECRM13516_RS17465 (ECRM13516_3454) - 3411818..3412054 (-) 237 WP_000562979.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  ECRM13516_RS17470 (ECRM13516_3455) - 3412065..3413492 (-) 1428 WP_025380569.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  ECRM13516_RS17475 (ECRM13516_3456) - 3413492..3414085 (-) 594 WP_000767721.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  ECRM13516_RS17480 (ECRM13516_3457) - 3414231..3414638 (+) 408 WP_001208062.1 MarR family transcriptional regulator -
  ECRM13516_RS17485 (ECRM13516_3458) rpoS 3414758..3415750 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  ECRM13516_RS17490 (ECRM13516_3459) nlpD 3415813..3416907 (-) 1095 WP_306174120.1 murein hydrolase activator NlpD -
  ECRM13516_RS17495 (ECRM13516_3460) pcm 3417092..3417718 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  ECRM13516_RS17500 (ECRM13516_3461) surE 3417712..3418473 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  ECRM13516_RS17505 (ECRM13516_3462) truD 3418454..3419503 (-) 1050 WP_000568924.1 tRNA pseudouridine(13) synthase TruD -
  ECRM13516_RS17510 (ECRM13516_3463) ispF 3419500..3419979 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ECRM13516_RS17515 (ECRM13516_3464) ispD 3419979..3420689 (-) 711 WP_000246160.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=100289 ECRM13516_RS17485 WP_000081550.1 3414758..3415750(-) (rpoS) [Escherichia coli O145:H28 str. RM13516]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=100289 ECRM13516_RS17485 WP_000081550.1 3414758..3415750(-) (rpoS) [Escherichia coli O145:H28 str. RM13516]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715

Multiple sequence alignment