Detailed information    

experimental Experimentally validated

Overview

Name   rpoS   Type   Regulator
Locus tag   GTF74_RS11590 Genome accession   NZ_CP047303
Coordinates   2562473..2563480 (-) Length   335 a.a.
NCBI ID   WP_000116737.1    Uniprot ID   O51804
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   regulation of chitinases   
Competence regulation

Function

An rpoS mutant has reduced chitinase activity, which is required to liberate the soluble chitin oligosaccharides that serve as an inducing cue for competence. RpoS is required for growth of V. cholerae on insoluble chitin. RpoS also regulates the mucosal escape response in pathogenic strains of V. cholerae.


Genomic Context

Location: 2557473..2568480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS11580 (GTF74_11585) cysM 2558637..2559524 (+) 888 WP_001279365.1 cysteine synthase CysM -
  GTF74_RS11585 (GTF74_11590) mutS 2559792..2562380 (+) 2589 WP_001894770.1 DNA mismatch repair protein MutS -
  GTF74_RS11590 (GTF74_11595) rpoS 2562473..2563480 (-) 1008 WP_000116737.1 RNA polymerase sigma factor RpoS Regulator
  GTF74_RS11595 (GTF74_11600) nlpD 2563554..2564489 (-) 936 WP_000177568.1 murein hydrolase activator NlpD -
  GTF74_RS11600 (GTF74_11605) - 2564489..2565115 (-) 627 WP_000002982.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  GTF74_RS11605 (GTF74_11610) surE 2565108..2565860 (-) 753 WP_000698379.1 5'/3'-nucleotidase SurE -
  GTF74_RS11610 (GTF74_11615) truD 2565862..2566947 (-) 1086 WP_000129752.1 tRNA pseudouridine(13) synthase TruD -
  GTF74_RS11615 (GTF74_11620) ispF 2566977..2567453 (-) 477 WP_000619209.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GTF74_RS11620 (GTF74_11625) ispD 2567450..2568148 (-) 699 WP_001061951.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GTF74_RS11625 (GTF74_11630) ftsB 2568145..2568429 (-) 285 WP_001263189.1 cell division protein FtsB -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 38534.39 Da        Isoelectric Point: 4.3648

>NTDB_id=1183 GTF74_RS11590 WP_000116737.1 2562473..2563480(-) (rpoS) [Vibrio cholerae O1 biovar El Tor strain E7946]
MSVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLY
ARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALM
NQTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVK
QGLNMEALFNVEYDN

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=1183 GTF74_RS11590 WP_000116737.1 2562473..2563480(-) (rpoS) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGAGTGTCAGCAATACCGTAACCAAAGTAGAAGAGTTCGATTTTGAAGATGAAGCACTGGAAGTGCTAGAAACTGATGC
CGAGCTCACCAGTGATGAAGAATTAGTTGCTGTTGAAGGGGCAAGTGAAGACGTTCGTGAAGAGTTTGATGCTTCTGCGA
AAAGTCTTGATGCGACCCAGATGTATCTCAGCGAAATTGGTTTTTCACCGCTCCTTACTGCCGAAGAAGAAGTGCTTTAT
GCTCGTCGTGCCTTACGTGGTGATGAAGCCGCACGTAAACGCATGATTGAAAGTAACTTGCGTCTGGTGGTAAAAATTTC
ACGCCGTTACAGCAACCGAGGATTAGCACTGCTCGATCTGATTGAAGAAGGTAATCTTGGTCTGATCCGTGCGGTTGAGA
AATTCGATCCAGAACGCGGTTTCCGCTTCTCTACCTACGCAACATGGTGGATCCGTCAAACCATTGAACGTGCGCTGATG
AACCAAACACGCACCATTCGTCTACCGATTCATGTCGTCAAAGAGCTGAACATTTATTTGCGTACTGCTCGCGAATTATC
ACAGCGCCTTGACCACGAACCTACACCAGAAGAAATTGCTCTTGAGTTAGACCGACCTGTCGATGATGTCACTAAGATGC
TGCGTCTTAACGAACGGATCAGCTCAGTGGATACGCCAATTGGTGGGGATGGAGATAAGGCACTGCTGGATATTTTGCCA
GACTCTCACAATGCCGATCCTGAGTTTTCAACTCAAGATGATGACATTCGTGAATCGCTGCTCAACTGGTTGGATGAACT
TAATCCAAAGCAAAAAGAAGTGCTTGCGCGTCGCTTTGGGCTTCTTGGCTATGAACCATCGACCTTGGAAGAAGTGGGTC
GTGAGATCAATCTCACTCGTGAGCGTGTTCGCCAAATCCAAGTGGAAGGTCTACGTCGTCTGCGTGAGATTTTGGTGAAA
CAAGGTTTGAATATGGAAGCGCTGTTTAACGTCGAATACGACAACTAA

Domains

Predicted by InterproScan.

Sigma-70, region 4 (Sigma70_r4)

(263-316)

Sigma-70 region 3 (Sigma70_r3)

(175-249)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(57-90)

Sigma-70 region 2 (Sigma70_r2)

(95-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O51804

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Ankur B Dalia (2016) RpoS is required for natural transformation of Vibrio cholerae through regulation of chitinases. Environmental Microbiology 18(11):3758-3767. [PMID: 26999629]