Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CV024_RS02450 Genome accession   NZ_CP156745
Coordinates   516214..517950 (+) Length   578 a.a.
NCBI ID   WP_257937331.1    Uniprot ID   -
Organism   Vibrio fluvialis strain QY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511214..522950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV024_RS02430 (CV024_02430) pilM 513520..514524 (+) 1005 WP_100267595.1 type IV pilus assembly protein PilM Machinery gene
  CV024_RS02435 (CV024_02435) pilN 514514..515104 (+) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  CV024_RS02440 (CV024_02440) pilO 515097..515687 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  CV024_RS02445 (CV024_02445) pilP 515677..516189 (+) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  CV024_RS02450 (CV024_02450) pilQ 516214..517950 (+) 1737 WP_257937331.1 type IV pilus secretin PilQ family protein Machinery gene
  CV024_RS02455 (CV024_02455) aroK 518138..518656 (+) 519 WP_004728569.1 shikimate kinase AroK -
  CV024_RS02460 (CV024_02460) aroB 518684..519769 (+) 1086 WP_020329448.1 3-dehydroquinate synthase -
  CV024_RS02465 (CV024_02465) - 519815..521323 (+) 1509 WP_100267597.1 AAA family ATPase -
  CV024_RS02470 (CV024_02470) - 521418..522248 (+) 831 WP_100267598.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62396.32 Da        Isoelectric Point: 4.5373

>NTDB_id=1002448 CV024_RS02450 WP_257937331.1 516214..517950(+) (pilQ) [Vibrio fluvialis strain QY27]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQEAAANQLKNIDFRVNKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1002448 CV024_RS02450 WP_257937331.1 516214..517950(+) (pilQ) [Vibrio fluvialis strain QY27]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTTGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCGTCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCCTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGAGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGTACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGCTGGGGTTTTAGCTCCACCAA
TGGCAGCACGACCACGGGGGGCTCAATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAATGTTAACCTCGCAGCCACCTCAGCTAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTCGGCGCCGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACCACCAACAAGCAGCCGGCGTATATTGAACAGGGTACTGAGATTCCGTACTTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCCTTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCGCAAATCACCCCCGAT
AACCGTCTGGTGCTCGATCTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGCACTGGCGAGGCGGTGGC
GATCAACACGCAACGTATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCCGTGTTAGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTTGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.865

100

0.82
  pilQ Vibrio cholerae strain A1552

81.865

100

0.82
  pilQ Vibrio campbellii strain DS40M4

79.235

94.983

0.753
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382

Multiple sequence alignment