Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEB33_RS01905 Genome accession   NZ_CP156025
Coordinates   417578..418717 (-) Length   379 a.a.
NCBI ID   WP_051156715.1    Uniprot ID   -
Organism   Herbaspirillum huttiense strain CLJ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412578..423717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEB33_RS01875 (ABEB33_01870) - 413126..413551 (+) 426 WP_039790450.1 NUDIX domain-containing protein -
  ABEB33_RS01880 (ABEB33_01875) yacG 413594..413779 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  ABEB33_RS01885 (ABEB33_01880) zapD 413815..414570 (-) 756 WP_034336663.1 cell division protein ZapD -
  ABEB33_RS01890 (ABEB33_01885) coaE 414784..415404 (-) 621 WP_343741380.1 dephospho-CoA kinase -
  ABEB33_RS01895 (ABEB33_01890) - 415409..416272 (-) 864 WP_402709157.1 prepilin peptidase -
  ABEB33_RS01900 (ABEB33_01895) - 416326..417591 (-) 1266 WP_402709155.1 type II secretion system F family protein -
  ABEB33_RS01905 (ABEB33_01900) pilF 417578..418717 (-) 1140 WP_051156715.1 GspE/PulE family protein Machinery gene
  ABEB33_RS01910 (ABEB33_01905) - 418868..420160 (-) 1293 WP_402709153.1 HlyC/CorC family transporter -
  ABEB33_RS01915 (ABEB33_01910) - 420334..421296 (-) 963 WP_402709151.1 2-hydroxyacid dehydrogenase -
  ABEB33_RS01920 (ABEB33_01915) - 421293..422150 (-) 858 WP_039790457.1 IclR family transcriptional regulator -
  ABEB33_RS01925 (ABEB33_01920) - 422417..423502 (+) 1086 WP_034336645.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 40828.48 Da        Isoelectric Point: 8.2654

>NTDB_id=1001328 ABEB33_RS01905 WP_051156715.1 417578..418717(-) (pilF) [Herbaspirillum huttiense strain CLJ01]
MLHTPDTAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATRLKILARLDI
AEKRLPQDGKMRLALGTRTVDFRVSTLPTQFGEKIVLRILDTAQAALSISDLGYEPAQQEALLQAISKPHGLVLMTGPTG
SGKTVSLYACLQRLNQPGVNIATAEDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKA
SQTGHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCRCRRPAGGTPATFVPHGCPACNQTGFRGRT
GIYQVMPVSAAMQSLILAQAGTLELGRQAQAEGIIDLRRAGLLKVLRGETSVGEILACT

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1001328 ABEB33_RS01905 WP_051156715.1 417578..418717(-) (pilF) [Herbaspirillum huttiense strain CLJ01]
ATGCTGCACACTCCCGACACGGCCGTGGCCGATGATGATGTTCCCGTGGTGCGCCTGCTGCACAAGCTGCTGGCCGATGC
CGTCGGCATGGGCGCTTCCGACCTGCATTTCGAGCCCTTCGAACATCTCTTCCGCGTGCGCTTGCGGCTGGATGGCGTGC
TGCATGAACTGGCGCAGCCGCCGCTGAGCTTGCGCGACAAGCTGGCCACCCGCCTGAAGATCCTGGCGCGTCTGGATATC
GCCGAAAAGCGCCTGCCCCAAGACGGCAAGATGCGCCTGGCACTGGGCACGCGCACGGTGGATTTCCGCGTCTCGACGCT
GCCCACGCAGTTCGGTGAAAAAATCGTGCTGCGTATCCTCGATACCGCCCAGGCGGCGTTGTCGATCAGCGACCTCGGCT
ATGAACCGGCCCAGCAGGAAGCGCTGCTGCAGGCCATCAGCAAGCCGCACGGGCTGGTCCTGATGACCGGGCCGACCGGC
TCCGGCAAGACGGTCTCGCTGTATGCCTGTCTGCAGCGCCTGAACCAGCCGGGGGTGAACATCGCCACCGCCGAGGACCC
GGTGGAAATCAACCTGCCCGGCATCAACCAGGTCAGCATCAACGAACGCGCCGGACTGGACTTCGCCCTGGCACTGCGCG
CCTTCCTGCGCCAGGATCCGGATGTGCTGATGGTAGGCGAGATCCGCGACCTGGAGACCGCCGATATCGCCGTCAAGGCT
TCGCAGACCGGGCATCTGGTGCTCTCCACCCTGCACACCAACGACGCCCCGGCCACGCTCACGCGGCTGCTCAACATGGG
CGTGCCGGCCTACAACATTGCGGCATCGGTCAGCCTGATCGTGGCCCAGCGGCTGGTGCGCAAGCTGTGCCGCTGCCGCC
GTCCGGCCGGTGGTACGCCGGCCACCTTCGTGCCGCACGGCTGCCCGGCCTGCAACCAGACCGGCTTTCGCGGGCGCACC
GGCATCTATCAGGTGATGCCGGTCAGCGCGGCCATGCAGTCGCTGATCCTGGCCCAGGCCGGCACATTGGAACTGGGCCG
GCAGGCGCAAGCGGAGGGCATCATCGACCTGCGGCGGGCGGGACTGCTGAAGGTCTTGCGCGGTGAAACCAGCGTGGGAG
AAATCCTTGCCTGTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(23-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.298

100

0.578
  pilB Acinetobacter baylyi ADP1

55.325

100

0.562
  pilB Acinetobacter baumannii D1279779

54.381

100

0.557
  pilB Vibrio campbellii strain DS40M4

54.974

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

54.45

100

0.549
  pilB Vibrio cholerae strain A1552

54.047

100

0.546
  pilB Legionella pneumophila strain ERS1305867

51.282

100

0.528
  pilB Haemophilus influenzae Rd KW20

47.215

99.472

0.47
  pilB Haemophilus influenzae 86-028NP

46.95

99.472

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

42.118

100

0.451
  pilF Thermus thermophilus HB27

43.377

100

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.092

100

0.435
  pilB Glaesserella parasuis strain SC1401

49.057

83.905

0.412
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.633

100

0.398

Multiple sequence alignment