Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 252733..253531 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag ECOLIN_RS01240 Protein ID WP_000854730.1
Coordinates 252733..253110 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag ECOLIN_RS01245 Protein ID WP_001285481.1
Coordinates 253157..253531 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS27255 (ECOLIN_01165) 247839..249243 + 1405 Protein_227 IS66 family transposase -
ECOLIN_RS01210 (ECOLIN_01170) 249497..250630 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
ECOLIN_RS01215 (ECOLIN_01175) 250691..250819 + 129 Protein_229 transposase domain-containing protein -
ECOLIN_RS01220 (ECOLIN_01180) 250803..250991 + 189 Protein_230 IS66 family transposase -
ECOLIN_RS01225 (ECOLIN_01185) 251139..252127 + 989 Protein_231 IS630 family transposase -
ECOLIN_RS27590 252206..252701 - 496 Protein_232 hypothetical protein -
ECOLIN_RS01240 (ECOLIN_01200) 252733..253110 - 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
ECOLIN_RS01245 (ECOLIN_01205) 253157..253531 - 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECOLIN_RS01250 (ECOLIN_01210) 253581..254225 - 645 WP_000086759.1 hypothetical protein -
ECOLIN_RS01255 (ECOLIN_01215) 254244..254465 - 222 WP_000692315.1 DUF987 domain-containing protein -
ECOLIN_RS01260 (ECOLIN_01220) 254528..255004 - 477 WP_038427078.1 RadC family protein -
ECOLIN_RS01265 (ECOLIN_01225) 255020..255493 - 474 WP_001313575.1 antirestriction protein -
ECOLIN_RS01270 (ECOLIN_01230) 255587..255832 - 246 WP_001164966.1 hypothetical protein -
ECOLIN_RS01275 (ECOLIN_01235) 255832..256653 - 822 WP_024199417.1 DUF945 domain-containing protein -
ECOLIN_RS01280 (ECOLIN_01240) 256753..256986 - 234 WP_001213776.1 DUF905 family protein -
ECOLIN_RS01285 (ECOLIN_01245) 257104..257556 - 453 WP_000682723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 243683..255832 12149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T47027 WP_000854730.1 NZ_CP007799:c253110-252733 [Escherichia coli Nissle 1917]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T47027 NZ_CP007799:c253110-252733 [Escherichia coli Nissle 1917]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT47027 WP_001285481.1 NZ_CP007799:c253531-253157 [Escherichia coli Nissle 1917]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT47027 NZ_CP007799:c253531-253157 [Escherichia coli Nissle 1917]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References