Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4032510..4033308 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag K3072_RS19420 Protein ID WP_000854730.1
Coordinates 4032931..4033308 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag K3072_RS19415 Protein ID WP_001285481.1
Coordinates 4032510..4032884 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS19375 (4028485) 4028485..4028937 + 453 WP_000682723.1 hypothetical protein -
K3072_RS19380 (4029055) 4029055..4029288 + 234 WP_001213776.1 DUF905 family protein -
K3072_RS19385 (4029388) 4029388..4030209 + 822 WP_001234359.1 DUF932 domain-containing protein -
K3072_RS19390 (4030209) 4030209..4030454 + 246 WP_001164966.1 hypothetical protein -
K3072_RS19395 (4030548) 4030548..4031021 + 474 WP_000855076.1 antirestriction protein -
K3072_RS19400 (4031037) 4031037..4031513 + 477 WP_001186170.1 RadC family protein -
K3072_RS19405 (4031576) 4031576..4031797 + 222 WP_000692315.1 DUF987 domain-containing protein -
K3072_RS19410 (4031816) 4031816..4032460 + 645 WP_000086761.1 hypothetical protein -
K3072_RS19415 (4032510) 4032510..4032884 + 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K3072_RS19420 (4032931) 4032931..4033308 + 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
K3072_RS19425 (4033305) 4033305..4033797 + 493 Protein_3824 DUF5983 family protein -
K3072_RS19430 (4033876) 4033876..4034864 - 989 Protein_3825 IS630 family transposase -
K3072_RS19435 (4035012) 4035012..4035200 - 189 Protein_3826 IS66 family transposase -
K3072_RS19440 (4035261) 4035261..4036394 + 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
K3072_RS19445 (4036648) 4036648..4038052 - 1405 Protein_3828 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4032510..4042997 10487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T212572 WP_000854730.1 NZ_CP081010:4032931-4033308 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T212572 NZ_CP107284:909056-909463 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGTCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT212572 WP_001285481.1 NZ_CP081010:4032510-4032884 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT212572 NZ_CP107284:908809-909075 [Escherichia coli]
ATGTACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References