20570

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4415059..4415648 Replicon chromosome
Accession NC_002947
Organism Pseudomonas putida KT2440

Toxin (Protein)


Gene name hicA Uniprot ID Q88G26
Locus tag PP_RS20310 Protein ID WP_010954726.1
Coordinates 4415400..4415648 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2V4F1F2
Locus tag PP_RS20305 Protein ID WP_049587764.1
Coordinates 4415059..4415400 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PP_RS20270 (PP_3894) 4410310..4411140 - 831 WP_010954720.1 replication protein -
PP_RS20275 (PP_3895) 4411465..4411677 - 213 WP_010954721.1 helix-turn-helix domain-containing protein -
PP_RS20280 (PP_3896) 4411765..4412412 + 648 WP_010954722.1 helix-turn-helix transcriptional regulator -
PP_RS20285 (PP_3897) 4412470..4412883 + 414 WP_010954723.1 HIRAN domain-containing protein -
PP_RS20290 (PP_3898) 4412927..4413163 + 237 WP_010954724.1 hypothetical protein -
PP_RS20295 (PP_5643) 4413330..4414322 + 993 WP_049587768.1 hypothetical protein -
PP_RS20300 (PP_5644) 4414325..4414891 + 567 WP_049587766.1 hypothetical protein -
PP_RS20305 (PP_3899) 4415059..4415400 - 342 WP_049587764.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PP_RS20310 (PP_3900) 4415400..4415648 - 249 WP_010954726.1 type II toxin-antitoxin system HicA family toxin Toxin
PP_RS20315 (PP_3901) 4415798..4416058 + 261 WP_010954727.1 DUF1654 domain-containing protein -
PP_RS20320 (PP_3903) 4416430..4416639 + 210 WP_049587762.1 hypothetical protein -
PP_RS20325 (PP_3905) 4417276..4417659 + 384 WP_049587761.1 helix-turn-helix transcriptional regulator -
PP_RS20330 (PP_3906) 4417679..4417948 + 270 WP_010954732.1 hypothetical protein -
PP_RS20335 (PP_3907) 4417945..4418403 + 459 WP_010954733.1 hypothetical protein -
PP_RS20340 (PP_3908) 4418485..4418835 + 351 WP_010954734.1 hypothetical protein -
PP_RS20345 (PP_3909) 4418849..4419127 - 279 WP_010954735.1 hypothetical protein -
PP_RS20350 (PP_3910) 4419234..4419785 + 552 WP_049587760.1 hypothetical protein -
PP_RS20355 (PP_3911) 4419800..4420183 + 384 WP_010954737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4377646..4431298 53652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9137.67 Da        Isoelectric Point: 10.4156

>T20570 WP_010954726.1 NC_002947:c4415648-4415400 [Pseudomonas putida KT2440]
MSKQEKLLAKLLNKQAGFTWPELVTLLGGLGYRHIEGDGSRVKFDNGHPQAMINLHKPHPGNELKAYVKRQVIEHLKAGG
LI

Download         Length: 249 bp

>T20570 NC_002947:c4415648-4415400 [Pseudomonas putida KT2440]
ATGTCTAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCTGAGCTGGTCACGCTGCT
GGGTGGGCTTGGATACCGCCATATCGAGGGTGATGGCAGCCGAGTGAAGTTCGACAATGGCCACCCGCAGGCAATGATCA
ACTTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTAATAGAGCACCTGAAAGCAGGAGGA
TTGATCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12402.90 Da        Isoelectric Point: 5.0719

>AT20570 WP_049587764.1 NC_002947:c4415400-4415059 [Pseudomonas putida KT2440]
MSTMLQYRGYYGSIEASPEDNCLFGKLQFIRALVSYEGETVAELTQAFRDAVDDYLDTCASLGQNPEIPCKGSFNVRVGH
DLHLAASVAATKQSISLNDLTRKALSEYLGHHA

Download         Length: 342 bp

>AT20570 NC_002947:c4415400-4415059 [Pseudomonas putida KT2440]
ATGAGCACCATGTTGCAGTACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAGGACAATTGCCTATTCGGCAAGCT
TCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCTGAACTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTATCTCGACACCTGCGCATCACTCGGGCAGAACCCGGAAATTCCGTGCAAGGGGTCGTTCAATGTGAGGGTAGGGCAC
GACCTGCACCTAGCGGCCAGCGTGGCAGCCACCAAGCAAAGTATTTCCTTGAATGATCTGACACGCAAAGCGCTTAGTGA
GTACCTGGGTCATCACGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88G26

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F1F2

References