119874

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1778811..1779400 Replicon chromosome
Accession NZ_CP036494
Organism Pseudomonas putida strain PP1

Toxin (Protein)


Gene name hicA Uniprot ID Q88G26
Locus tag EZZ80_RS08065 Protein ID WP_010954726.1
Coordinates 1778811..1779059 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2V4F1F2
Locus tag EZZ80_RS08070 Protein ID WP_049587764.1
Coordinates 1779059..1779400 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EZZ80_RS08020 (EZZ80_08210) 1774276..1774659 - 384 WP_010954737.1 hypothetical protein -
EZZ80_RS08025 (EZZ80_08215) 1774674..1775225 - 552 WP_049587760.1 hypothetical protein -
EZZ80_RS08030 (EZZ80_08220) 1775332..1775610 + 279 WP_010954735.1 hypothetical protein -
EZZ80_RS08035 (EZZ80_08225) 1775624..1775974 - 351 WP_010954734.1 hypothetical protein -
EZZ80_RS08040 (EZZ80_08230) 1776056..1776514 - 459 WP_010954733.1 hypothetical protein -
EZZ80_RS08045 (EZZ80_08235) 1776511..1776780 - 270 WP_010954732.1 hypothetical protein -
EZZ80_RS08050 (EZZ80_08240) 1776800..1777183 - 384 WP_049587761.1 helix-turn-helix transcriptional regulator -
EZZ80_RS08055 (EZZ80_08250) 1777820..1778029 - 210 WP_049587762.1 hypothetical protein -
EZZ80_RS08060 (EZZ80_08260) 1778401..1778661 - 261 WP_010954727.1 DUF1654 domain-containing protein -
EZZ80_RS08065 (EZZ80_08265) 1778811..1779059 + 249 WP_010954726.1 type II toxin-antitoxin system HicA family toxin Toxin
EZZ80_RS08070 (EZZ80_08270) 1779059..1779400 + 342 WP_049587764.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EZZ80_RS08075 (EZZ80_08275) 1779568..1780134 - 567 WP_049587766.1 hypothetical protein -
EZZ80_RS08080 (EZZ80_08280) 1780137..1781129 - 993 WP_049587768.1 hypothetical protein -
EZZ80_RS08085 (EZZ80_08285) 1781296..1781532 - 237 WP_010954724.1 hypothetical protein -
EZZ80_RS08090 (EZZ80_08290) 1781576..1781989 - 414 WP_010954723.1 HIRAN domain-containing protein -
EZZ80_RS08095 (EZZ80_08295) 1782047..1782694 - 648 WP_010954722.1 S24 family peptidase -
EZZ80_RS08100 (EZZ80_08300) 1782782..1782994 + 213 WP_010954721.1 YdaS family helix-turn-helix protein -
EZZ80_RS08105 (EZZ80_08305) 1783319..1784149 + 831 WP_010954720.1 replication protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1754427..1816813 62386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9137.67 Da        Isoelectric Point: 10.4156

>T119874 WP_010954726.1 NZ_CP036494:1778811-1779059 [Pseudomonas putida]
MSKQEKLLAKLLNKQAGFTWPELVTLLGGLGYRHIEGDGSRVKFDNGHPQAMINLHKPHPGNELKAYVKRQVIEHLKAGG
LI

Download         Length: 249 bp

>T119874 NZ_CP036494:1778811-1779059 [Pseudomonas putida]
ATGTCTAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCTGAGCTGGTCACGCTGCT
GGGTGGGCTTGGATACCGCCATATCGAGGGTGATGGCAGCCGAGTGAAGTTCGACAATGGCCACCCGCAGGCAATGATCA
ACTTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTAATAGAGCACCTGAAAGCAGGAGGA
TTGATCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12402.90 Da        Isoelectric Point: 5.0719

>AT119874 WP_049587764.1 NZ_CP036494:1779059-1779400 [Pseudomonas putida]
MSTMLQYRGYYGSIEASPEDNCLFGKLQFIRALVSYEGETVAELTQAFRDAVDDYLDTCASLGQNPEIPCKGSFNVRVGH
DLHLAASVAATKQSISLNDLTRKALSEYLGHHA

Download         Length: 342 bp

>AT119874 NZ_CP036494:1779059-1779400 [Pseudomonas putida]
ATGAGCACCATGTTGCAGTACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAGGACAATTGCCTATTCGGCAAGCT
TCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCTGAACTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTATCTCGACACCTGCGCATCACTCGGGCAGAACCCGGAAATTCCGTGCAAGGGGTCGTTCAATGTGAGGGTAGGGCAC
GACCTGCACCTAGCGGCCAGCGTGGCAGCCACCAAGCAAAGTATTTCCTTGAATGATCTGACACGCAAAGCGCTTAGTGA
GTACCTGGGTCATCACGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88G26

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F1F2

References