Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4415103..4415692 Replicon chromosome
Accession NZ_CP054264
Organism Pseudomonas putida strain A0_F0_I1

Toxin (Protein)


Gene name hicA Uniprot ID Q88G26
Locus tag HG554_RS20375 Protein ID WP_010954726.1
Coordinates 4415444..4415692 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2V4F1F2
Locus tag HG554_RS20370 Protein ID WP_049587764.1
Coordinates 4415103..4415444 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG554_RS20335 (HG554_20330) 4410354..4411184 - 831 WP_010954720.1 replication protein -
HG554_RS20340 (HG554_20335) 4411509..4411721 - 213 WP_010954721.1 YdaS family helix-turn-helix protein -
HG554_RS20345 (HG554_20340) 4411809..4412456 + 648 WP_010954722.1 S24 family peptidase -
HG554_RS20350 (HG554_20345) 4412514..4412927 + 414 WP_010954723.1 HIRAN domain-containing protein -
HG554_RS20355 (HG554_20350) 4412971..4413207 + 237 WP_010954724.1 hypothetical protein -
HG554_RS20360 (HG554_20355) 4413374..4414366 + 993 WP_049587768.1 hypothetical protein -
HG554_RS20365 (HG554_20360) 4414369..4414935 + 567 WP_049587766.1 hypothetical protein -
HG554_RS20370 (HG554_20365) 4415103..4415444 - 342 WP_049587764.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HG554_RS20375 (HG554_20370) 4415444..4415692 - 249 WP_010954726.1 type II toxin-antitoxin system HicA family toxin Toxin
HG554_RS20380 (HG554_20375) 4415842..4416102 + 261 WP_010954727.1 DUF1654 domain-containing protein -
HG554_RS20385 (HG554_20380) 4416474..4416683 + 210 WP_049587762.1 hypothetical protein -
HG554_RS20390 (HG554_20385) 4417320..4417703 + 384 WP_049587761.1 helix-turn-helix transcriptional regulator -
HG554_RS20395 (HG554_20390) 4417723..4417992 + 270 WP_010954732.1 hypothetical protein -
HG554_RS20400 (HG554_20395) 4417989..4418447 + 459 WP_010954733.1 hypothetical protein -
HG554_RS20405 (HG554_20400) 4418529..4418879 + 351 WP_010954734.1 hypothetical protein -
HG554_RS20410 (HG554_20405) 4418893..4419171 - 279 WP_010954735.1 hypothetical protein -
HG554_RS20415 (HG554_20410) 4419278..4419829 + 552 WP_049587760.1 hypothetical protein -
HG554_RS20420 (HG554_20415) 4419844..4420227 + 384 WP_010954737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4377690..4440076 62386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9137.67 Da        Isoelectric Point: 10.4156

>T159236 WP_010954726.1 NZ_CP054264:c4415692-4415444 [Pseudomonas putida]
MSKQEKLLAKLLNKQAGFTWPELVTLLGGLGYRHIEGDGSRVKFDNGHPQAMINLHKPHPGNELKAYVKRQVIEHLKAGG
LI

Download         Length: 249 bp

>T159236 NZ_CP069990:659267-659521 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12402.90 Da        Isoelectric Point: 5.0719

>AT159236 WP_049587764.1 NZ_CP054264:c4415444-4415103 [Pseudomonas putida]
MSTMLQYRGYYGSIEASPEDNCLFGKLQFIRALVSYEGETVAELTQAFRDAVDDYLDTCASLGQNPEIPCKGSFNVRVGH
DLHLAASVAATKQSISLNDLTRKALSEYLGHHA

Download         Length: 342 bp

>AT159236 NZ_CP069990:659019-659270 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88G26

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F1F2

References