Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4415070..4415659 Replicon chromosome
Accession NZ_CP097527
Organism Pseudomonas putida strain KT2440 isolate pnCas9-BEC6-C

Toxin (Protein)


Gene name hicA Uniprot ID Q88G26
Locus tag M8001_RS20480 Protein ID WP_010954726.1
Coordinates 4415411..4415659 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2V4F1F2
Locus tag M8001_RS20475 Protein ID WP_049587764.1
Coordinates 4415070..4415411 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8001_RS20440 (M8001_20410) 4410321..4411151 - 831 WP_010954720.1 replication protein -
M8001_RS20445 (M8001_20415) 4411476..4411688 - 213 WP_010954721.1 YdaS family helix-turn-helix protein -
M8001_RS20450 (M8001_20420) 4411776..4412423 + 648 WP_010954722.1 S24 family peptidase -
M8001_RS20455 (M8001_20425) 4412481..4412894 + 414 WP_010954723.1 HIRAN domain-containing protein -
M8001_RS20460 (M8001_20430) 4412938..4413174 + 237 WP_010954724.1 hypothetical protein -
M8001_RS20465 (M8001_20435) 4413341..4414333 + 993 WP_049587768.1 hypothetical protein -
M8001_RS20470 (M8001_20440) 4414336..4414902 + 567 WP_049587766.1 hypothetical protein -
M8001_RS20475 (M8001_20445) 4415070..4415411 - 342 WP_049587764.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
M8001_RS20480 (M8001_20450) 4415411..4415659 - 249 WP_010954726.1 type II toxin-antitoxin system HicA family toxin Toxin
M8001_RS20485 (M8001_20455) 4415809..4416069 + 261 WP_010954727.1 DUF1654 domain-containing protein -
M8001_RS20490 (M8001_20460) 4416441..4416650 + 210 WP_049587762.1 hypothetical protein -
M8001_RS20495 (M8001_20465) 4417287..4417670 + 384 WP_049587761.1 helix-turn-helix transcriptional regulator -
M8001_RS20500 (M8001_20470) 4417690..4417959 + 270 WP_010954732.1 hypothetical protein -
M8001_RS20505 (M8001_20475) 4417956..4418414 + 459 WP_010954733.1 hypothetical protein -
M8001_RS20510 (M8001_20480) 4418496..4418846 + 351 WP_010954734.1 hypothetical protein -
M8001_RS20515 (M8001_20485) 4418860..4419138 - 279 WP_010954735.1 hypothetical protein -
M8001_RS20520 (M8001_20490) 4419245..4419796 + 552 WP_049587760.1 hypothetical protein -
M8001_RS20525 (M8001_20495) 4419811..4420194 + 384 WP_010954737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4377657..4431309 53652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9137.67 Da        Isoelectric Point: 10.4156

>T245527 WP_010954726.1 NZ_CP097527:c4415659-4415411 [Pseudomonas putida]
MSKQEKLLAKLLNKQAGFTWPELVTLLGGLGYRHIEGDGSRVKFDNGHPQAMINLHKPHPGNELKAYVKRQVIEHLKAGG
LI

Download         Length: 249 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12402.90 Da        Isoelectric Point: 5.0719

>AT245527 WP_049587764.1 NZ_CP097527:c4415411-4415070 [Pseudomonas putida]
MSTMLQYRGYYGSIEASPEDNCLFGKLQFIRALVSYEGETVAELTQAFRDAVDDYLDTCASLGQNPEIPCKGSFNVRVGH
DLHLAASVAATKQSISLNDLTRKALSEYLGHHA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88G26

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F1F2

References