Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yjhX-yjhQ/YjhX-GNAT
Location 4534430..4535244 Replicon chromosome
Accession NZ_CP056036
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name yjhX Uniprot ID S1PA82
Locus tag HUW39_RS21795 Protein ID WP_001054376.1
Coordinates 4534430..4534687 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name yjhQ Uniprot ID U9Z4B8
Locus tag HUW39_RS21800 Protein ID WP_001309181.1
Coordinates 4534699..4535244 (+) Length 182 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS21775 (4529690) 4529690..4530670 + 981 WP_000991438.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
HUW39_RS26445 (4530780) 4530780..4530985 + 206 Protein_4310 HNH endonuclease -
HUW39_RS21780 (4531285) 4531285..4532265 - 981 WP_000019400.1 IS5-like element IS5 family transposase -
HUW39_RS21785 (4532452) 4532452..4533692 - 1241 Protein_4312 helicase YjhR -
HUW39_RS26450 (4533808) 4533808..4533939 + 132 WP_001309182.1 hypothetical protein -
HUW39_RS21795 (4534430) 4534430..4534687 + 258 WP_001054376.1 YjhX family toxin Toxin
HUW39_RS21800 (4534699) 4534699..4535244 + 546 WP_001309181.1 N-acetyltransferase Antitoxin
HUW39_RS21805 (4535300) 4535300..4536046 + 747 WP_000354251.1 class I SAM-dependent methyltransferase -
HUW39_RS21810 (4536215) 4536215..4536433 + 219 Protein_4317 hypothetical protein -
HUW39_RS21815 (4536471) 4536471..4536587 + 117 Protein_4318 VOC family protein -
HUW39_RS21820 (4536832) 4536832..4537953 + 1122 WP_000010829.1 M42 family metallopeptidase -
HUW39_RS21825 (4537950) 4537950..4538228 + 279 WP_000722973.1 PTS sugar transporter subunit IIB SgcB -
HUW39_RS21830 (4538240) 4538240..4539553 + 1314 WP_000460843.1 PTS sugar transporter subunit IIC SgcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimI / fimA / fimE / fimB 4523017..4535244 12227
flank IS/Tn - - 4531285..4532265 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Putative toxin of bacterial toxin-antitoxin pair

(1-85)

Antitoxin

GNAT domain

(18-121)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9734.29 Da        Isoelectric Point: 11.0090

>T162541 WP_001054376.1 NZ_CP056036:4534430-4534687 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Download         Length: 258 bp

>T162541 NZ_CP071845:1423803-1424057 [Vibrio sp. SCSIO 43139]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTGGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAAGATGCTGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACCGTTCACT
TACTACGCTCTCCTCAAAACGCTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAACACGAGAG
TTAATTGATGAGTAG

Antitoxin


Download         Length: 182 a.a.        Molecular weight: 19956.90 Da        Isoelectric Point: 6.3277

>AT162541 WP_001309181.1 NZ_CP056036:4534699-4535244 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Download         Length: 546 bp

>AT162541 NZ_CP071845:1424050-1424319 [Vibrio sp. SCSIO 43139]
ATGAGTAGTAGTCAACGTTTACTATCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATGTAAAGCGCTCCCCATTTGAGGGTATCGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTGTTGATGACACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATCTCGTGTCGTTACCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PA82

Antitoxin

Source ID Structure
AlphaFold DB U9Z4B8

References