Detailed information of TA system TA010053

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/-
• Location: 926227..927335
• Replicon: chromosome
• Accession: NZ_CP007497
• Organism: Streptococcus suis strain ZY05719 isolate diseased pig

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: ZY05719_RS04600
• Protein ID: WP_000233000.1
• Coordinates: 926466..927335 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: ZY05719_RS04595
• Protein ID: WP_000205227.1
• Coordinates: 926227..926451 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZY05719_RS04575 (ZY05719_04600)	922167..923279	-	1113	WP_011922450.1	lantibiotic-modifying protein	-
ZY05719_RS04580 (ZY05719_04605)	923807..924103	+	297	WP_002360685.1	hypothetical protein	-
ZY05719_RS04585 (ZY05719_04610)	924104..924520	+	417	WP_000323438.1	recombinase	-
ZY05719_RS04590 (ZY05719_04615)	924522..926084	+	1563	WP_000136908.1	recombinase family protein	-
ZY05719_RS04595 (ZY05719_04620)	926227..926451	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
ZY05719_RS04600 (ZY05719_04625)	926466..927335	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
ZY05719_RS04605 (ZY05719_04630)	927316..928050	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
ZY05719_RS04610 (ZY05719_04635)	928083..928946	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
ZY05719_RS04615 (ZY05719_04640)	928990..929517	+	528	WP_002294507.1	adenine phosphoribosyltransferase	-
ZY05719_RS04620 (ZY05719_04645)	929745..931064	+	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_012924 Streptococcus suis SC84 Integrative and Conjugative Element TA023991, TA023992, TA023993, TA023994 808819..964460 0 View NC_012924 Streptococcus suis SC84 Integrative and Conjugative Element TA023991, TA023992, TA023993, TA023994 808819..964460 0 View NZ_CP018908 Streptococcus suis strain SS2-1 Integrative and Conjugative Element TA070792, TA070793, TA070794, TA070795 785098..940739 0 View NZ_CP020863 Streptococcus suis strain SC19 Integrative and Conjugative Element TA075581, TA075582, TA075583, TA075584 808893..964533 0	inside	Integrative and Conjugative Element	tet(M) / ant(6)-Ia	-	808718..964358	155640
	-	flank	IS/Tn	ant(6)-Ia	-	928083..931064	2981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T10053 WP_000233000.1 NZ_CP007497:926466-927335 [Streptococcus suis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T10053 NZ_CP007497:926466-927335 [Streptococcus suis]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT10053 WP_000205227.1 NZ_CP007497:926227-926451 [Streptococcus suis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT10053 NZ_CP007497:926227-926451 [Streptococcus suis]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1

References

(1) Qibing Gu et al. (2021) An Auto-Regulating Type II Toxin-Antitoxin System Modulates Drug Resistance and Virulence in Streptococcus suis. Frontiers in Microbiology 12:671706. [PubMed:34475853]


 experimental literature