Detailed information of regulator


Summary

Regulator ID REG0323 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Cationic antimicrobial peptide (CAMP) resistance (map01503)
Related T6SS T6SS01170 experimental (Type i1)
Strain Pectobacterium brasiliense strain 1692
Replicon chromosome
Sequence Protein sequence (230 a.a.); Nucleotide sequence (690 bp)
Description PhoP-dependent regulation of the T6SS gene cluster in Pb1692 primarily depends on the expR1 regulator.Pb1692 overexpresses expR1 along with 23 T6SS genes in the absence of phoP.
Reference
External database links

Locus tag (Gene) GT391_RS16925 (phoP)
Coordinates (Strand) 3795787..3796476 (-)
NCBI ID 497961604
RefSeq NZ_CP047495
Uniprot ID UPI0001A446E6
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020; map01503
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
GT391_RS06470 (expR1) ↑ 746272689
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 5.1e-24 3..112
Trans_reg_C PF00486.30 2.9e-21 147..220
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-229 MRILVVEDNVLLRHHLTVQMNEMGHQVDAASDAKEADYFLHENMPDIAIVDLGLPDEDGMSMIRRWRTHQVKLPILVLTAREGWQDKVAVLEAGADDYVTKPFHMEEVVARLQALMRRNSGLASQVISLPPFEIDLSRREMMIHSSPIKLTAFEYTIIETLIRNKGKVVSKESLMLQLYPDAELRESHTIDVLMGRLRKKIQQAEAPDVITTVRGQGYRFDIDPPSGSA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9922 - -


  Protein sequence of regulator: 230 a.a.    .

>REG0323 NZ_CP047495:c3796476-3795787 [Pectobacterium brasiliense]
MRILVVEDNVLLRHHLTVQMNEMGHQVDAASDAKEADYFLHENMPDIAIVDLGLPDEDGMSMIRRWRTHQVKLPILVLTA
REGWQDKVAVLEAGADDYVTKPFHMEEVVARLQALMRRNSGLASQVISLPPFEIDLSRREMMIHSSPIKLTAFEYTIIET
LIRNKGKVVSKESLMLQLYPDAELRESHTIDVLMGRLRKKIQQAEAPDVITTVRGQGYRFDIDPPSGSA*

  Nucleotide sequence of regulator: 690 bp    .

>REG0323 NZ_CP047495:c3796476-3795787 [Pectobacterium brasiliense]
ATGCGGATTCTGGTCGTTGAAGATAATGTATTATTGCGCCACCATTTAACCGTTCAGATGAACGAAATGGGGCATCAGGT
TGACGCGGCGTCTGACGCCAAAGAAGCGGATTATTTTCTGCATGAAAATATGCCGGATATCGCCATTGTCGACCTCGGGC
TACCTGATGAAGACGGCATGAGTATGATCCGTCGCTGGCGCACCCATCAGGTCAAGCTCCCGATTCTGGTGCTGACCGCC
CGTGAAGGCTGGCAGGACAAAGTTGCGGTGCTGGAAGCCGGTGCAGACGATTACGTGACGAAGCCGTTCCATATGGAAGA
AGTCGTGGCGCGCTTGCAGGCACTGATGCGGCGCAACAGCGGACTGGCCTCGCAAGTCATCAGCCTGCCGCCTTTTGAGA
TCGATTTATCGCGCCGCGAAATGATGATCCACAGCAGCCCGATCAAGCTCACCGCGTTTGAATACACGATTATCGAAACG
TTGATTCGTAACAAAGGCAAGGTCGTCAGCAAAGAGTCGCTGATGTTGCAGCTGTACCCCGATGCAGAACTGCGGGAAAG
CCACACCATCGACGTATTGATGGGGCGGCTACGCAAGAAAATTCAGCAGGCAGAAGCGCCGGATGTGATCACCACCGTAC
GCGGACAAGGATATCGCTTTGATATTGATCCCCCTTCAGGCTCAGCATGA