Detailed information of immunity protein
Summary
| Immunity protein ID | IMU38225  |
| Cognate effector | EFF76654 |
| Related T6SS (Type) | T6SS00164 (Type i3) |
| Strain | Acidovorax citrulli AAC00-1 |
| Replicon | chromosome |
| Sequence | Protein sequence (108 a.a.); Nucleotide sequence (324 bp) |
| Description | Deletion of rhsB significantly impairs the ability of killing Bacillus subtilis while ectopic expression of immunity proteins RimB1 or RimB2 confers protection. |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | AAVE_RS02480 (RimB1) |
| Coordinates (Strand) | 543696..544019 (+) |
| NCBI ID | WP_041827204.1 |
| RefSeq | NC_008752 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-107 | MQDFLVSPSLLDLYQATKSREWRAQVKAKITVIFKFLQDNNLANVRLLDESGEAIEDLVLMRSHLTEAGVKMFAKAIPAWQRARDKDGNLANIKLLEDGLKKILENG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9959 | - | - |
Protein sequence: 108 a.a. .
>IMU38225 NC_008752:543696-544019 [Acidovorax citrulli AAC00-1]
MQDFLVSPSLLDLYQATKSREWRAQVKAKITVIFKFLQDNNLANVRLLDESGEAIEDLVLMRSHLTEAGVKMFAKAIPAW
QRARDKDGNLANIKLLEDGLKKILENG*
MQDFLVSPSLLDLYQATKSREWRAQVKAKITVIFKFLQDNNLANVRLLDESGEAIEDLVLMRSHLTEAGVKMFAKAIPAW
QRARDKDGNLANIKLLEDGLKKILENG*
Nucleotide sequence: 324 bp .
>IMU38225 NC_008752:543696-544019 [Acidovorax citrulli AAC00-1]
ATGCAGGATTTTTTGGTATCCCCGAGTCTGTTGGATTTGTATCAGGCCACCAAGAGTAGGGAGTGGCGCGCACAGGTAAA
AGCTAAAATCACCGTTATATTTAAGTTTCTGCAGGACAATAATCTCGCCAATGTGAGGCTTCTTGATGAGTCGGGCGAGG
CGATTGAAGATCTGGTGCTGATGCGGTCACATCTCACAGAGGCAGGGGTGAAAATGTTTGCAAAGGCAATTCCTGCGTGG
CAGCGCGCACGCGATAAGGATGGGAATCTGGCGAACATCAAGTTGCTTGAGGATGGCCTGAAGAAGATATTGGAAAACGG
CTGA
ATGCAGGATTTTTTGGTATCCCCGAGTCTGTTGGATTTGTATCAGGCCACCAAGAGTAGGGAGTGGCGCGCACAGGTAAA
AGCTAAAATCACCGTTATATTTAAGTTTCTGCAGGACAATAATCTCGCCAATGTGAGGCTTCTTGATGAGTCGGGCGAGG
CGATTGAAGATCTGGTGCTGATGCGGTCACATCTCACAGAGGCAGGGGTGAAAATGTTTGCAAAGGCAATTCCTGCGTGG
CAGCGCGCACGCGATAAGGATGGGAATCTGGCGAACATCAAGTTGCTTGAGGATGGCCTGAAGAAGATATTGGAAAACGG
CTGA