Detailed information of immunity protein


Summary

Immunity protein ID IMU38209 experimental
Cognate effector EFF76634 experimental
Related T6SS (Type) T6SS01046 experimental (Type i4b)
Strain Acinetobacter baumannii strain ATCC 17978
Replicon chromosome
Sequence Protein sequence (269 a.a.); Nucleotide sequence (807 bp)
Description Overexpression of tsi4 (ACX60_00610) in M2ΔtssB prey prevented killing by Ab17978Δ123, indicating that Tsi4 is the immunity protein of Tse4
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ACX60_00605 (tsi4)
Coordinates (Strand) 133713..134519 (+)
NCBI ID WP_000598979.1
RefSeq NZ_CP012004
Uniprot ID A0A1E3MC10_ACIBA
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-268 MIKYKLCLALLLGASTVANAEFNIENNPNQPVYIKLGYQNVLNSSFIYTDVSKNVDLKKNIVTVPYGEVTVLLNPVLNNNNEIKGKDYYFINYQPKILLGKIQYKLPFVISDVGAIGTSIIYNDNNISIFSIESYANPVSNNNSKKFKSDIYIFNKKNLGIYNPSFLSINVKLKSLNDASMKFTKLSSIKYNKNDKSYQVVYDFQKVTEDFTSTGKVNYEKFPIQYSFSLIPDKNKPFLIKDIIEKRKGDKNIISKIENNDMYSYSLK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9974 pos: 20-21. ANA-EF. MIKYKLCLALLLGASTVANA


  Protein sequence: 269 a.a.    .

>IMU38209 NZ_CP012004:133713-134519 [Acinetobacter baumannii]
MIKYKLCLALLLGASTVANAEFNIENNPNQPVYIKLGYQNVLNSSFIYTDVSKNVDLKKNIVTVPYGEVTVLLNPVLNNN
NEIKGKDYYFINYQPKILLGKIQYKLPFVISDVGAIGTSIIYNDNNISIFSIESYANPVSNNNSKKFKSDIYIFNKKNLG
IYNPSFLSINVKLKSLNDASMKFTKLSSIKYNKNDKSYQVVYDFQKVTEDFTSTGKVNYEKFPIQYSFSLIPDKNKPFLI
KDIIEKRKGDKNIISKIENNDMYSYSLK*

  Nucleotide sequence: 807 bp    .

>IMU38209 NZ_CP012004:133713-134519 [Acinetobacter baumannii]
ATGATTAAGTATAAATTATGTCTGGCACTCCTATTGGGAGCCTCAACAGTAGCTAATGCTGAGTTTAATATAGAAAATAA
TCCAAATCAGCCTGTATATATCAAATTAGGGTATCAGAATGTCTTAAATTCTTCCTTTATATATACTGATGTCAGCAAAA
ATGTAGATTTGAAAAAAAATATCGTTACTGTTCCATATGGCGAAGTTACAGTTTTACTAAATCCAGTTTTGAATAATAAT
AATGAGATAAAAGGAAAGGATTATTACTTTATAAACTATCAACCAAAAATTCTATTAGGAAAAATTCAGTATAAGCTTCC
TTTTGTTATTTCAGATGTTGGAGCAATTGGTACTAGTATTATTTATAATGATAATAATATTTCTATTTTTTCAATTGAAA
GTTATGCAAATCCTGTGTCAAATAATAATTCAAAAAAATTTAAAAGTGATATATATATATTTAATAAAAAAAATTTAGGA
ATATATAATCCATCATTTTTATCAATAAATGTTAAATTAAAAAGTTTAAATGATGCTTCAATGAAGTTTACAAAATTATC
ATCTATAAAATATAATAAAAATGATAAGTCCTATCAAGTAGTATATGATTTTCAGAAAGTAACTGAAGATTTTACTTCAA
CTGGAAAAGTTAATTATGAGAAGTTTCCAATTCAATATTCTTTTTCCTTGATTCCTGATAAAAATAAACCTTTTTTAATT
AAAGATATTATTGAAAAGAGAAAGGGCGATAAAAATATTATTTCGAAAATTGAAAATAATGATATGTATTCATATTCTTT
AAAATAG