Detailed information of immunity protein
Summary
| Immunity protein ID | IMU17307  |
| Cognate effector | EFF18627 |
| Related T6SS (Type) | T6SS17501 (Type i3) |
| Strain | Neisseria cinerea isolate CCUG346T (346T) |
| Replicon | chromosome |
| Sequence | Protein sequence (212 a.a.); Nucleotide sequence (636 bp) |
| Description | All Ntes are toxic, with their expression leading to decreased viability and reduced optical density (OD) of E. coli cultures compared to empty vector controls; toxicity was counteracted by co-expression of the corresponding Nti. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | Nti5 |
| Coordinates (Strand) | 14672..15307 (-) |
| NCBI ID | - |
| GenBank | 346T_plasmid |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Imm43 | PF15570.8 | 2.4e-09 | 57..128 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-211 | MKKLLVWGYALQSMDKAEKMGLPRVSSGAVGISEKFNPKKTHPDFDFPWSWFVKGQEIPKMPEKVYVCLKKARSVTFDFLPYSDFFIMSERFLEFIKKYGFEHNFGMSKVVIVNTKGELLTDETYYLIRIVSWEIENEIVYPDLGLNEEDFSLEAYTNSDKDILLSSNHNYSGAFMVSENLKDKILENFSLPFLYSLAEWKELNNLDSYWD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.6661 | - | - |
Protein sequence: 212 a.a. .
>IMU17307 346T_plasmid:c15307-14672 [synthetic DNA construct]
MKKLLVWGYALQSMDKAEKMGLPRVSSGAVGISEKFNPKKTHPDFDFPWSWFVKGQEIPKMPEKVYVCLKKARSVTFDFL
PYSDFFIMSERFLEFIKKYGFEHNFGMSKVVIVNTKGELLTDETYYLIRIVSWEIENEIVYPDLGLNEEDFSLEAYTNSD
KDILLSSNHNYSGAFMVSENLKDKILENFSLPFLYSLAEWKELNNLDSYWD*
MKKLLVWGYALQSMDKAEKMGLPRVSSGAVGISEKFNPKKTHPDFDFPWSWFVKGQEIPKMPEKVYVCLKKARSVTFDFL
PYSDFFIMSERFLEFIKKYGFEHNFGMSKVVIVNTKGELLTDETYYLIRIVSWEIENEIVYPDLGLNEEDFSLEAYTNSD
KDILLSSNHNYSGAFMVSENLKDKILENFSLPFLYSLAEWKELNNLDSYWD*
Nucleotide sequence: 636 bp .
>IMU17307 346T_plasmid:c15307-14672 [synthetic DNA construct]
ATGAAAAAATTACTAGTATGGGGATATGCCCTACAAAGCATGGATAAAGCAGAAAAAATGGGGTTGCCACGAGTAAGTAG
TGGAGCTGTGGGTATATCAGAGAAGTTTAACCCTAAGAAAACGCATCCTGATTTTGACTTTCCGTGGAGTTGGTTTGTAA
AGGGACAAGAAATCCCGAAAATGCCTGAAAAAGTTTATGTCTGCTTAAAAAAAGCAAGGAGTGTTACTTTTGATTTTCTG
CCATATAGTGACTTCTTTATTATGTCAGAGCGTTTTTTGGAATTTATTAAAAAATACGGGTTTGAACATAATTTTGGCAT
GAGCAAGGTAGTGATTGTCAATACAAAAGGCGAGCTATTGACAGATGAAACCTATTATTTGATAAGAATAGTTAGCTGGG
AAATTGAGAATGAAATTGTTTACCCAGATTTAGGATTAAATGAAGAAGATTTTTCGCTTGAAGCATACACTAATTCGGAT
AAGGATATTTTATTGTCTTCCAATCATAATTATTCAGGAGCATTTATGGTGTCAGAAAATTTAAAAGACAAAATATTGGA
AAACTTTTCTTTGCCTTTTCTTTATTCTTTGGCAGAGTGGAAGGAATTGAACAATTTAGACAGTTATTGGGATTAA
ATGAAAAAATTACTAGTATGGGGATATGCCCTACAAAGCATGGATAAAGCAGAAAAAATGGGGTTGCCACGAGTAAGTAG
TGGAGCTGTGGGTATATCAGAGAAGTTTAACCCTAAGAAAACGCATCCTGATTTTGACTTTCCGTGGAGTTGGTTTGTAA
AGGGACAAGAAATCCCGAAAATGCCTGAAAAAGTTTATGTCTGCTTAAAAAAAGCAAGGAGTGTTACTTTTGATTTTCTG
CCATATAGTGACTTCTTTATTATGTCAGAGCGTTTTTTGGAATTTATTAAAAAATACGGGTTTGAACATAATTTTGGCAT
GAGCAAGGTAGTGATTGTCAATACAAAAGGCGAGCTATTGACAGATGAAACCTATTATTTGATAAGAATAGTTAGCTGGG
AAATTGAGAATGAAATTGTTTACCCAGATTTAGGATTAAATGAAGAAGATTTTTCGCTTGAAGCATACACTAATTCGGAT
AAGGATATTTTATTGTCTTCCAATCATAATTATTCAGGAGCATTTATGGTGTCAGAAAATTTAAAAGACAAAATATTGGA
AAACTTTTCTTTGCCTTTTCTTTATTCTTTGGCAGAGTGGAAGGAATTGAACAATTTAGACAGTTATTGGGATTAA