Detailed information of immunity protein


Summary

Immunity protein ID IMU17306 experimental
Cognate effector EFF18626 experimental
Related T6SS (Type) T6SS17501 experimental (Type i3)
Strain Neisseria cinerea isolate CCUG346T (346T)
Replicon chromosome
Sequence Protein sequence (194 a.a.); Nucleotide sequence (582 bp)
Description All Ntes are toxic, with their expression leading to decreased viability and reduced optical density (OD) of E. coli cultures compared to empty vector controls; toxicity was counteracted by co-expression of the corresponding Nti.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) Nti4
Coordinates (Strand) 17614..18195 (-)
NCBI ID -
GenBank 346T_plasmid
Uniprot ID -
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Imm26 PF15428.8 1e-06 17..99

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-193 MKIFNWEKNRTLYRYLKNGDLFCFKIADKLFGYGRIIAKNKLGATVGIFDLFTSSPVEPFDYKNAGNYPILFKTVLDCHTLFESKLESDWRIIAQDPDYQDPTPSEITSINPAMGLAHNADFSIEQEIDEEDGMQKILKSIELLETPNSHYHILSFLIRRKPEIFNLPIESFEEFGDFISDEFQEIHHRNLKA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9695 - -



  Protein sequence: 194 a.a.    .

>IMU17306 346T_plasmid:c18195-17614 [synthetic DNA construct]
MKIFNWEKNRTLYRYLKNGDLFCFKIADKLFGYGRIIAKNKLGATVGIFDLFTSSPVEPFDYKNAGNYPILFKTVLDCHT
LFESKLESDWRIIAQDPDYQDPTPSEITSINPAMGLAHNADFSIEQEIDEEDGMQKILKSIELLETPNSHYHILSFLIRR
KPEIFNLPIESFEEFGDFISDEFQEIHHRNLKA*

  Nucleotide sequence: 582 bp    .

>IMU17306 346T_plasmid:c18195-17614 [synthetic DNA construct]
ATGAAAATCTTTAATTGGGAAAAAAACAGAACTTTATATAGATATCTTAAGAATGGTGATTTATTTTGTTTTAAAATAGC
TGATAAATTATTCGGTTACGGTAGAATAATCGCTAAAAATAAGCTTGGTGCCACTGTAGGAATTTTTGATTTGTTTACTT
CAAGCCCAGTTGAGCCATTCGATTATAAAAATGCTGGGAATTACCCAATTCTATTCAAAACCGTTTTAGACTGTCATACC
TTATTTGAATCAAAGCTAGAGTCAGACTGGCGGATAATTGCTCAAGATCCCGATTACCAAGATCCAACCCCAAGTGAAAT
TACTTCTATTAATCCTGCTATGGGATTGGCTCACAATGCAGACTTTTCCATCGAGCAAGAAATAGATGAAGAGGATGGTA
TGCAAAAAATACTCAAGAGCATTGAATTGCTTGAAACACCCAATAGTCATTATCATATTTTAAGTTTTCTTATTCGTCGT
AAACCAGAAATTTTTAATTTACCTATTGAATCGTTTGAAGAATTTGGAGATTTTATTTCGGATGAGTTTCAAGAAATTCA
CCACCGGAATTTAAAAGCGTAA