Detailed information of effector


Summary

Effector ID EFF18626 experimental
Effector type Tde
Cognate immunity protein IMU17306 experimental
Related T6SS (Type) T6SS17501 experimental (Type i3)
Strain Neisseria cinerea isolate CCUG346T (346T)
Replicon chromosome
Sequence Protein sequence (978 a.a.); Nucleotide sequence (2934 bp)
Description Nte4 contains a GIY-YIG nuclease domain (cd00719). All Ntes are toxic, with their expression leading to decreased viability and reduced optical density (OD) of E. coli cultures compared to empty vector controls; toxicity was counteracted by co-expression of the corresponding Nti.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) Nte4
Coordinates (Strand) 18226..21159 (-)
NCBI ID -
GenBank 346T_plasmid
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
RHS PF03527.16 1.3e-11 743..775
RHS_repeat PF05593.16 1.5e-08 129..158
RHS_repeat PF05593.16 9e-07 228..264
RHS_repeat PF05593.16 4.2e-06 81..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-977 MVEHNQPDGLVSRYEYDRYDTDGKVLKSSNNLGEEWTFDYRKDHTVVTDALGRTEVYGFDENHELVYRIDADGQRSDSERDSYGRITVERDPLGRETRYLYDTEGNVIAITAPDGGSTQIDYHETLNLPVAVNDPAGRITAYTYDGRGNLVSITDPAGYTTRYGYNARWLPETITDALGKTRRLHYDTLDQLVCFTDCTGETTRFAYTEYGDLETVTDALGHTTRHHYDAAGNPVRTDYPDGSHETFEYDRLNRLTAHIDGLGAQTAYELAVDGLPLKRTNALGHTFAYAYDKARRLTVLTNENGETYRLDYGPTDNLIQETGWDGKITAYGYDAAGQLVRQTEYGQSTDQGRLKDRPETWHIHRFKRNNLGQLIEKQSRKVSGRNGQSKDEGISRTRFDYDPITGNLTKARNRHSSVELAYDELDHLIGETTVHNGQSATVGYQYDPLGNRIRTILPDGRHIDYLYYGSGHLHQISLDGEVITDIERDKLHREIQRTQGGISSLYDYDPMGRLKSQRTVRSGTQTPRGKQNPPAGGAVNRRYAYDKAGNLIQSADQRSGVLHYVYDKIGRIQEARNSQTGRSETFAFDPAHNILSDKTAEGKGKGSNISSGNRLESYNGIEYTYDALGNLIYRQLPNGENQYYQYDLENQLVRAEIKKPAGNTEIWTYAYDPFGRRLSKERQDKLSWTSTDPKRTHFVWDGTRLLQEYTYKGSYTYLYTDQDSYEPLAQIFDNAKDSKQYLAYFHNDQIGIPREMTDIHGNLLWYGEYTAWGRLKKDGRVYQHAHQPFRLQNQYYDEETGLHYNLMRYYEPEAGRFVNQDPIGLDGGANFYQFATNAQIWLDPLGLKKYSVYGLYRPGDSKPFYIGITDNIKTRAKQHRKSGRLVGTERLVELYSDLDYETARGYEEGCIRQHGTLTGKKIKKGQKSNFNPKKTKTRGNKCHSFVENKTRNAKRQEAMVAGRDHILKNGCKNLPII


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9910 - -


  Protein sequence: 978 a.a.    .

>EFF18626 346T_plasmid:c21159-18226 Type VI DNase and RNase Effector (Tde) [synthetic DNA construct]
MVEHNQPDGLVSRYEYDRYDTDGKVLKSSNNLGEEWTFDYRKDHTVVTDALGRTEVYGFDENHELVYRIDADGQRSDSER
DSYGRITVERDPLGRETRYLYDTEGNVIAITAPDGGSTQIDYHETLNLPVAVNDPAGRITAYTYDGRGNLVSITDPAGYT
TRYGYNARWLPETITDALGKTRRLHYDTLDQLVCFTDCTGETTRFAYTEYGDLETVTDALGHTTRHHYDAAGNPVRTDYP
DGSHETFEYDRLNRLTAHIDGLGAQTAYELAVDGLPLKRTNALGHTFAYAYDKARRLTVLTNENGETYRLDYGPTDNLIQ
ETGWDGKITAYGYDAAGQLVRQTEYGQSTDQGRLKDRPETWHIHRFKRNNLGQLIEKQSRKVSGRNGQSKDEGISRTRFD
YDPITGNLTKARNRHSSVELAYDELDHLIGETTVHNGQSATVGYQYDPLGNRIRTILPDGRHIDYLYYGSGHLHQISLDG
EVITDIERDKLHREIQRTQGGISSLYDYDPMGRLKSQRTVRSGTQTPRGKQNPPAGGAVNRRYAYDKAGNLIQSADQRSG
VLHYVYDKIGRIQEARNSQTGRSETFAFDPAHNILSDKTAEGKGKGSNISSGNRLESYNGIEYTYDALGNLIYRQLPNGE
NQYYQYDLENQLVRAEIKKPAGNTEIWTYAYDPFGRRLSKERQDKLSWTSTDPKRTHFVWDGTRLLQEYTYKGSYTYLYT
DQDSYEPLAQIFDNAKDSKQYLAYFHNDQIGIPREMTDIHGNLLWYGEYTAWGRLKKDGRVYQHAHQPFRLQNQYYDEET
GLHYNLMRYYEPEAGRFVNQDPIGLDGGANFYQFATNAQIWLDPLGLKKYSVYGLYRPGDSKPFYIGITDNIKTRAKQHR
KSGRLVGTERLVELYSDLDYETARGYEEGCIRQHGTLTGKKIKKGQKSNFNPKKTKTRGNKCHSFVENKTRNAKRQEAMV
AGRDHILKNGCKNLPII*

  Nucleotide sequence: 2934 bp    .

>EFF18626 346T_plasmid:c21159-18226 Type VI DNase and RNase Effector (Tde) [synthetic DNA construct]
ATGGTCGAACACAACCAGCCCGACGGACTGGTATCCCGCTACGAATACGACCGTTACGACACCGACGGCAAAGTGCTCAA
AAGCAGCAACAACCTCGGCGAAGAATGGACGTTCGACTACCGCAAAGACCATACCGTCGTTACCGACGCATTGGGACGGA
CGGAAGTGTACGGTTTCGACGAAAACCACGAACTCGTCTACCGCATCGACGCCGACGGACAACGCAGCGACAGCGAACGC
GACAGCTACGGCCGCATTACCGTAGAACGCGACCCCCTCGGACGCGAAACCCGCTATCTCTACGATACCGAAGGCAACGT
CATCGCCATCACCGCCCCCGACGGCGGCAGCACCCAAATCGACTACCACGAAACCCTCAACCTGCCGGTTGCCGTCAACG
ATCCCGCCGGCAGGATTACCGCCTACACCTACGACGGACGCGGCAACCTCGTCAGCATCACCGATCCCGCCGGTTACACC
ACCCGCTACGGCTACAACGCCCGATGGCTGCCCGAAACTATTACCGACGCCTTAGGCAAAACCCGACGCCTACACTACGA
CACCCTCGACCAACTCGTCTGCTTTACCGACTGTACCGGCGAAACCACCCGCTTCGCTTATACCGAATACGGCGATCTCG
AAACCGTTACCGATGCGCTGGGCCATACCACCCGCCACCACTACGACGCAGCGGGCAACCCCGTCCGTACCGACTATCCC
GACGGCAGTCACGAAACCTTCGAATACGACCGCCTCAACCGTCTGACCGCCCACATCGACGGACTCGGTGCCCAAACCGC
CTACGAACTCGCAGTGGACGGACTGCCGCTCAAACGCACCAACGCACTGGGCCATACCTTCGCCTACGCCTACGACAAAG
CCCGCCGTCTGACCGTCCTCACCAACGAAAACGGCGAAACCTACCGTCTCGATTACGGCCCGACCGACAACCTGATCCAA
GAAACCGGCTGGGACGGCAAAATCACCGCCTACGGCTACGATGCCGCCGGACAACTGGTCCGGCAGACCGAATACGGCCA
AAGCACCGATCAAGGCCGTCTGAAAGACCGACCCGAGACTTGGCACATCCACCGGTTCAAACGCAACAACCTCGGCCAAC
TGATCGAAAAACAAAGCCGCAAAGTCTCCGGCCGCAACGGACAAAGCAAAGACGAAGGCATCAGCCGCACCCGTTTCGAC
TACGACCCCATTACCGGCAACCTGACCAAAGCCCGCAACCGACACAGCAGCGTCGAACTCGCCTACGACGAACTCGACCA
CCTCATCGGCGAAACCACCGTCCACAACGGCCAAAGCGCCACCGTAGGCTATCAATACGACCCGCTGGGCAACCGCATCC
GCACCATCCTGCCCGACGGCCGCCATATCGATTACCTGTACTACGGCAGCGGACACCTGCACCAAATCAGCCTAGACGGC
GAAGTCATCACCGACATCGAACGCGACAAACTGCACCGCGAAATCCAAAGGACGCAGGGCGGCATCAGCAGCCTGTACGA
CTACGACCCCATGGGTCGTCTGAAAAGCCAGCGTACCGTCCGCAGCGGCACACAAACACCCCGCGGCAAACAAAACCCGC
CGGCCGGCGGCGCCGTCAACCGCCGCTACGCCTACGACAAAGCCGGCAACCTGATTCAAAGTGCCGACCAAAGAAGCGGC
GTCCTTCATTACGTTTACGACAAAATCGGACGGATTCAGGAAGCCCGCAACAGCCAAACCGGCCGCAGCGAAACCTTCGC
CTTCGACCCCGCCCACAACATCCTTTCCGACAAAACAGCCGAAGGGAAGGGCAAAGGCAGTAACATTAGCAGCGGCAACC
GCCTAGAATCATACAACGGCATCGAATACACCTACGACGCACTGGGCAACCTGATCTACCGCCAGCTGCCCAACGGTGAA
AACCAATATTACCAATACGATTTAGAAAACCAACTCGTCCGCGCCGAAATCAAAAAGCCCGCCGGCAACACCGAGATCTG
GACCTACGCCTACGACCCGTTCGGCAGACGCCTTTCCAAAGAACGCCAAGACAAACTCTCCTGGACGAGCACCGACCCCA
AACGCACCCACTTCGTCTGGGACGGAACACGATTACTTCAGGAGTACACCTACAAAGGCAGCTATACCTATCTCTACACC
GACCAAGACAGCTACGAACCGCTGGCGCAAATCTTTGATAACGCCAAAGACAGCAAACAATACCTCGCCTATTTCCACAA
CGACCAAATCGGCATACCGAGGGAGATGACCGACATCCACGGCAATCTCTTATGGTACGGCGAATACACCGCCTGGGGTC
GTCTGAAAAAGGACGGGCGGGTCTATCAGCACGCGCATCAGCCGTTTAGATTGCAGAACCAGTATTATGATGAAGAGACC
GGGCTGCATTACAATCTGATGCGCTATTACGAGCCTGAGGCGGGGCGGTTTGTGAATCAGGATCCGATTGGGTTAGATGG
TGGAGCTAATTTTTATCAATTTGCCACCAATGCTCAAATTTGGTTAGATCCATTGGGATTAAAAAAATATTCTGTTTATG
GATTGTATAGGCCAGGGGATAGTAAACCTTTTTATATCGGTATTACGGATAATATAAAAACGCGTGCAAAACAACACAGA
AAATCAGGAAGATTAGTTGGAACAGAACGCTTAGTAGAATTATATAGCGATTTGGATTACGAAACCGCCCGTGGATATGA
GGAAGGCTGTATTCGACAACATGGAACACTTACAGGAAAAAAAATTAAAAAAGGTCAGAAAAGTAATTTTAATCCTAAAA
AAACTAAAACCAGAGGAAATAAATGTCATTCATTTGTTGAGAATAAGACCCGTAATGCTAAAAGACAAGAAGCAATGGTT
GCGGGAAGAGACCATATTCTCAAAAATGGTTGTAAAAATCTTCCAATAATTTAA