Detailed information of immunity protein
Summary
| Immunity protein ID | IMU17305  |
| Cognate effector | EFF18625 |
| Related T6SS (Type) | T6SS17501 (Type i3) |
| Strain | Neisseria cinerea isolate CCUG346T (346T) |
| Replicon | chromosome |
| Sequence | Protein sequence (145 a.a.); Nucleotide sequence (435 bp) |
| Description | All Ntes are toxic, with their expression leading to decreased viability and reduced optical density (OD) of E. coli cultures compared to empty vector controls; toxicity was counteracted by co-expression of the corresponding Nti. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | Nti3 |
| Coordinates (Strand) | 21474..21908 (-) |
| NCBI ID | - |
| GenBank | 346T_plasmid |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| SUKH_5 | PF14567.8 | 5.3e-19 | 7..141 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-144 | MISQETMNLLIKYFQDYPELKGIPASSEEIKHAENILGIKFHDDYIEFIKKFGGASAGLDIHAFHNSTLIGKETVIELTQGFQKLLELHEQPMNKLYAISDDGSGNPILMDQQGHIFIYYHDSSEMEMLYESLDSLFKETLLGE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9924 | - | - |
Protein sequence: 145 a.a. .
>IMU17305 346T_plasmid:c21908-21474 [synthetic DNA construct]
MISQETMNLLIKYFQDYPELKGIPASSEEIKHAENILGIKFHDDYIEFIKKFGGASAGLDIHAFHNSTLIGKETVIELTQ
GFQKLLELHEQPMNKLYAISDDGSGNPILMDQQGHIFIYYHDSSEMEMLYESLDSLFKETLLGE*
MISQETMNLLIKYFQDYPELKGIPASSEEIKHAENILGIKFHDDYIEFIKKFGGASAGLDIHAFHNSTLIGKETVIELTQ
GFQKLLELHEQPMNKLYAISDDGSGNPILMDQQGHIFIYYHDSSEMEMLYESLDSLFKETLLGE*
Nucleotide sequence: 435 bp .
>IMU17305 346T_plasmid:c21908-21474 [synthetic DNA construct]
ATGATTAGTCAAGAAACAATGAATTTACTTATTAAATATTTTCAAGATTACCCTGAGCTTAAAGGGATACCCGCTAGTAG
TGAGGAAATTAAACATGCTGAAAATATTTTAGGAATAAAATTTCACGATGATTACATTGAATTTATTAAAAAATTCGGCG
GCGCAAGCGCTGGTTTGGATATCCATGCTTTCCATAATTCAACACTAATTGGAAAAGAAACAGTTATCGAATTAACTCAG
GGATTTCAAAAACTTTTAGAATTACATGAACAACCAATGAATAAACTTTATGCAATATCAGATGATGGTTCAGGCAATCC
CATTTTAATGGATCAACAGGGACATATTTTTATTTATTATCATGATTCATCGGAAATGGAAATGTTATATGAATCACTTG
ATAGCTTATTTAAAGAAACATTGTTGGGTGAATAG
ATGATTAGTCAAGAAACAATGAATTTACTTATTAAATATTTTCAAGATTACCCTGAGCTTAAAGGGATACCCGCTAGTAG
TGAGGAAATTAAACATGCTGAAAATATTTTAGGAATAAAATTTCACGATGATTACATTGAATTTATTAAAAAATTCGGCG
GCGCAAGCGCTGGTTTGGATATCCATGCTTTCCATAATTCAACACTAATTGGAAAAGAAACAGTTATCGAATTAACTCAG
GGATTTCAAAAACTTTTAGAATTACATGAACAACCAATGAATAAACTTTATGCAATATCAGATGATGGTTCAGGCAATCC
CATTTTAATGGATCAACAGGGACATATTTTTATTTATTATCATGATTCATCGGAAATGGAAATGTTATATGAATCACTTG
ATAGCTTATTTAAAGAAACATTGTTGGGTGAATAG