SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU17304
Cognate effector	EFF18624
Related T6SS (Type)	T6SS17501 (Type i3)
Strain	Neisseria cinerea isolate CCUG346T (346T)
Replicon	chromosome
Sequence	Protein sequence (271 a.a.); Nucleotide sequence (813 bp)
Description	All Ntes are toxic, with their expression leading to decreased viability and reduced optical density (OD) of E. coli cultures compared to empty vector controls; toxicity was counteracted by co-expression of the corresponding Nti.
Reference	[1] Custodio R, Ford RM, Ellison CJ, et al. Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili. Elife. 2021 Jul 7;10. pii: e63755. doi: 10.7554/eLife.63755. PMID: 34232858

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	Nti2
Coordinates (Strand)	57364..58176 (+)
NCBI ID	-
GenBank	346T_plasmid
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-270	MVGKEVSSFDAFLVCKQLSVKELFEKILNSNTVFQYEAAKRLQFYEYNEIKDDIKNILLTSRYSRHREIAIFILGQFQIKLNDIQLKEILSILICFIQNDKSIRVKSSAISSLGYLFRDYNLGEKEFSNIEKDIDLIWSLNNYSIIISIAFSSIYIPEREYIKDYLVRNLNKKNTKILSWILYSLKEKGYKSNSIETLLIRKLKDFNETSYIYNEIVLFLVSIDSKKVIPYVKKILLNQNRIDDEFYIEIKNNSSKKFSKIRKILLKKFE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9941	-	-

Protein sequence: 271 a.a. .

                                    >IMU17304 346T_plasmid:57364-58176 [synthetic DNA construct]
MVGKEVSSFDAFLVCKQLSVKELFEKILNSNTVFQYEAAKRLQFYEYNEIKDDIKNILLTSRYSRHREIAIFILGQFQIK
LNDIQLKEILSILICFIQNDKSIRVKSSAISSLGYLFRDYNLGEKEFSNIEKDIDLIWSLNNYSIIISIAFSSIYIPERE
YIKDYLVRNLNKKNTKILSWILYSLKEKGYKSNSIETLLIRKLKDFNETSYIYNEIVLFLVSIDSKKVIPYVKKILLNQN
RIDDEFYIEIKNNSSKKFSKIRKILLKKFE*

                                

Nucleotide sequence: 813 bp .

                                    >IMU17304 346T_plasmid:57364-58176 [synthetic DNA construct]
ATGGTAGGCAAAGAAGTTTCAAGTTTTGATGCTTTTCTAGTATGCAAGCAATTGTCAGTTAAGGAGTTATTCGAAAAAAT
ACTTAATTCAAATACTGTTTTTCAATATGAAGCAGCAAAAAGGTTACAATTTTATGAGTATAATGAAATTAAAGATGATA
TTAAAAATATACTATTAACAAGCAGATATAGTCGACATAGAGAAATTGCAATATTTATTTTAGGACAGTTTCAAATCAAA
CTTAATGATATTCAGCTTAAAGAAATTCTATCAATATTAATTTGCTTTATACAAAATGATAAAAGCATAAGAGTTAAATC
ATCTGCAATTTCATCATTGGGATACCTATTTCGTGATTATAATTTAGGAGAAAAAGAGTTTTCTAACATAGAAAAAGATA
TAGATTTGATATGGAGTTTAAATAATTATTCTATTATTATTTCTATTGCATTTAGTTCTATATATATTCCAGAAAGGGAA
TATATAAAAGACTATCTAGTTAGAAATTTAAATAAAAAAAATACTAAGATATTGTCATGGATTTTATATTCTTTAAAAGA
GAAAGGTTATAAATCAAATTCAATTGAAACTCTATTAATTAGGAAGCTAAAAGATTTTAATGAAACTTCTTATATTTACA
ATGAGATAGTTTTATTTTTAGTTTCAATTGACTCTAAGAAGGTTATTCCGTATGTTAAAAAAATTTTATTGAATCAAAAT
AGAATTGATGATGAATTCTATATTGAAATCAAGAATAATTCATCTAAAAAGTTTTCAAAAATTAGAAAAATACTGCTAAA
GAAATTTGAATAA

                                