Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00385  |
| Cognate effector | EFF01895 |
| Related T6SS (Type) | T6SS01301 (Type i3) |
| Strain | Burkholderia gladioli NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (194 a.a.); Nucleotide sequence (582 bp) |
| Description | Immunity protein represses the expression of the operon via binding to both external and internal promoters. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ACI79_RS32200 (TsiTBg2) |
| Coordinates (Strand) | 23400..23981 (-) |
| NCBI ID | 920807827 |
| GenBank | LEKY01000082 |
| Uniprot ID | UPI00069CDC85 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Tox-REase-5 | PF15648.8 | 2e-32 | 80..172 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-193 | MAVPLIEGAAMEFGPLLMRIGAALLGGAGTASLSGDATDDDIEARASAHAVPRTAERCERCPPESGLAVKRFHSMNAAPREYQGRVTGRPYSVAEGWSEEWAWLNIDFDGFVPGQCLLQEAKGNYDQFLDGSIPAAYKFFKGFESMEAAAEKQASLVRAKPPTRLMWYFQTPLAQSRMMLTLRHLGIPSIVHP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| TAT(Tat/SPI) | 0.7192 | pos: 37-38. GDA-TD. | MAVPLIEGAAMEFGPLLMRIGAALLGGAGTASLSGDA |
Protein sequence: 194 a.a. .
>IMU00385 LEKY01000082:c23981-23400 [Burkholderia gladioli]
MAVPLIEGAAMEFGPLLMRIGAALLGGAGTASLSGDATDDDIEARASAHAVPRTAERCERCPPESGLAVKRFHSMNAAPR
EYQGRVTGRPYSVAEGWSEEWAWLNIDFDGFVPGQCLLQEAKGNYDQFLDGSIPAAYKFFKGFESMEAAAEKQASLVRAK
PPTRLMWYFQTPLAQSRMMLTLRHLGIPSIVHP*
MAVPLIEGAAMEFGPLLMRIGAALLGGAGTASLSGDATDDDIEARASAHAVPRTAERCERCPPESGLAVKRFHSMNAAPR
EYQGRVTGRPYSVAEGWSEEWAWLNIDFDGFVPGQCLLQEAKGNYDQFLDGSIPAAYKFFKGFESMEAAAEKQASLVRAK
PPTRLMWYFQTPLAQSRMMLTLRHLGIPSIVHP*
Nucleotide sequence: 582 bp .
>IMU00385 LEKY01000082:c23981-23400 [Burkholderia gladioli]
ATGGCAGTACCTCTCATCGAAGGCGCCGCAATGGAATTTGGGCCGCTCTTGATGCGTATTGGCGCGGCGCTGTTGGGCGG
AGCAGGAACCGCTAGCCTGTCCGGCGACGCAACGGACGACGATATCGAGGCAAGGGCATCGGCACATGCGGTCCCGCGCA
CGGCAGAGCGTTGCGAACGCTGCCCACCCGAGTCCGGCTTGGCTGTCAAACGCTTCCACTCGATGAACGCGGCACCGCGC
GAATACCAGGGGCGCGTTACCGGGCGGCCTTATAGCGTCGCCGAAGGCTGGAGTGAGGAGTGGGCGTGGCTGAATATCGA
CTTCGACGGCTTCGTGCCGGGTCAATGCTTGTTGCAGGAAGCGAAAGGCAACTACGATCAGTTTCTCGATGGCTCTATCC
CTGCAGCCTATAAATTCTTCAAGGGATTCGAATCGATGGAGGCGGCAGCGGAGAAACAGGCCAGCCTTGTTCGTGCCAAG
CCGCCAACCCGCCTGATGTGGTATTTCCAGACACCTCTCGCACAGAGTCGCATGATGCTCACCTTGCGGCACCTTGGCAT
ACCGTCAATCGTCCACCCTTGA
ATGGCAGTACCTCTCATCGAAGGCGCCGCAATGGAATTTGGGCCGCTCTTGATGCGTATTGGCGCGGCGCTGTTGGGCGG
AGCAGGAACCGCTAGCCTGTCCGGCGACGCAACGGACGACGATATCGAGGCAAGGGCATCGGCACATGCGGTCCCGCGCA
CGGCAGAGCGTTGCGAACGCTGCCCACCCGAGTCCGGCTTGGCTGTCAAACGCTTCCACTCGATGAACGCGGCACCGCGC
GAATACCAGGGGCGCGTTACCGGGCGGCCTTATAGCGTCGCCGAAGGCTGGAGTGAGGAGTGGGCGTGGCTGAATATCGA
CTTCGACGGCTTCGTGCCGGGTCAATGCTTGTTGCAGGAAGCGAAAGGCAACTACGATCAGTTTCTCGATGGCTCTATCC
CTGCAGCCTATAAATTCTTCAAGGGATTCGAATCGATGGAGGCGGCAGCGGAGAAACAGGCCAGCCTTGTTCGTGCCAAG
CCGCCAACCCGCCTGATGTGGTATTTCCAGACACCTCTCGCACAGAGTCGCATGATGCTCACCTTGCGGCACCTTGGCAT
ACCGTCAATCGTCCACCCTTGA