Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00384  |
| Cognate effector | EFF01900 |
| Related T6SS (Type) | T6SS01301 (Type i3) |
| Strain | Burkholderia gladioli NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (222 a.a.); Nucleotide sequence (666 bp) |
| Description | Ectopic expression of TseTBg3 protein inhibited the growth, while co-expression of the cognate immunity (TsiTBg3) protein failed to protect the cells from effector mediated killing. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ACI79_RS22675 (TsiTBg3) |
| Coordinates (Strand) | 197212..197877 (-) |
| NCBI ID | 920807205 |
| GenBank | LEKY01000067 |
| Uniprot ID | UPI00069E6298 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Imm52 | PF15579.8 | 1.2e-25 | 114..215 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-221 | MLLNKVGAVIDVIATSEPQLSRSSWRMKGYSLEAAQSREVYEADGTPSSTAIETLRRERADESIASLAIWGGQVNQEFGASIEVLACGGDFPDTVTIEVRGAFVDAKKDAVVAIVARAALEFMPTAISATPKGYEEQQAFDDRPGVGWMLYLPVELTAQQIPEAWELIPVVSADGGRRLGTIIVSIEDEPFSIDNEAHLVAAHDIEVRLISMDLLPRFTDI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9668 | - | - |
Protein sequence: 222 a.a. .
>IMU00384 LEKY01000067:c197877-197212 [Burkholderia gladioli]
MLLNKVGAVIDVIATSEPQLSRSSWRMKGYSLEAAQSREVYEADGTPSSTAIETLRRERADESIASLAIWGGQVNQEFGA
SIEVLACGGDFPDTVTIEVRGAFVDAKKDAVVAIVARAALEFMPTAISATPKGYEEQQAFDDRPGVGWMLYLPVELTAQQ
IPEAWELIPVVSADGGRRLGTIIVSIEDEPFSIDNEAHLVAAHDIEVRLISMDLLPRFTDI*
MLLNKVGAVIDVIATSEPQLSRSSWRMKGYSLEAAQSREVYEADGTPSSTAIETLRRERADESIASLAIWGGQVNQEFGA
SIEVLACGGDFPDTVTIEVRGAFVDAKKDAVVAIVARAALEFMPTAISATPKGYEEQQAFDDRPGVGWMLYLPVELTAQQ
IPEAWELIPVVSADGGRRLGTIIVSIEDEPFSIDNEAHLVAAHDIEVRLISMDLLPRFTDI*
Nucleotide sequence: 666 bp .
>IMU00384 LEKY01000067:c197877-197212 [Burkholderia gladioli]
ATGCTCTTGAACAAGGTCGGTGCGGTCATCGACGTTATCGCGACCAGTGAGCCGCAACTGTCGCGATCGTCCTGGCGCAT
GAAGGGCTATTCGCTCGAAGCGGCGCAAAGCAGGGAGGTCTACGAGGCAGATGGCACACCGAGCTCGACAGCAATTGAGA
CGTTGCGGCGCGAGCGTGCCGACGAATCGATAGCGTCGCTTGCTATATGGGGTGGCCAGGTCAATCAGGAATTCGGTGCG
TCGATCGAGGTTCTGGCGTGCGGTGGGGATTTTCCCGACACAGTCACGATCGAGGTCCGGGGGGCGTTTGTCGACGCAAA
GAAGGATGCTGTCGTGGCCATCGTGGCGAGAGCCGCGCTGGAATTCATGCCAACGGCAATTTCAGCGACGCCGAAAGGCT
ATGAGGAGCAGCAGGCATTCGACGATCGCCCCGGCGTGGGCTGGATGCTGTACCTCCCCGTCGAACTGACCGCGCAACAG
ATTCCGGAGGCATGGGAACTGATCCCGGTCGTCTCGGCCGATGGCGGGCGACGCCTTGGCACGATCATCGTCAGCATCGA
GGACGAGCCGTTTTCGATCGACAACGAAGCGCACCTGGTAGCGGCGCACGATATCGAGGTGCGGCTCATTTCCATGGATC
TGCTGCCGCGCTTCACCGACATTTAA
ATGCTCTTGAACAAGGTCGGTGCGGTCATCGACGTTATCGCGACCAGTGAGCCGCAACTGTCGCGATCGTCCTGGCGCAT
GAAGGGCTATTCGCTCGAAGCGGCGCAAAGCAGGGAGGTCTACGAGGCAGATGGCACACCGAGCTCGACAGCAATTGAGA
CGTTGCGGCGCGAGCGTGCCGACGAATCGATAGCGTCGCTTGCTATATGGGGTGGCCAGGTCAATCAGGAATTCGGTGCG
TCGATCGAGGTTCTGGCGTGCGGTGGGGATTTTCCCGACACAGTCACGATCGAGGTCCGGGGGGCGTTTGTCGACGCAAA
GAAGGATGCTGTCGTGGCCATCGTGGCGAGAGCCGCGCTGGAATTCATGCCAACGGCAATTTCAGCGACGCCGAAAGGCT
ATGAGGAGCAGCAGGCATTCGACGATCGCCCCGGCGTGGGCTGGATGCTGTACCTCCCCGTCGAACTGACCGCGCAACAG
ATTCCGGAGGCATGGGAACTGATCCCGGTCGTCTCGGCCGATGGCGGGCGACGCCTTGGCACGATCATCGTCAGCATCGA
GGACGAGCCGTTTTCGATCGACAACGAAGCGCACCTGGTAGCGGCGCACGATATCGAGGTGCGGCTCATTTCCATGGATC
TGCTGCCGCGCTTCACCGACATTTAA