Detailed information of effector
Summary
| Effector ID | EFF01900  |
| Effector type | Tde |
| Cognate immunity protein | IMU00384 |
| Related T6SS (Type) | T6SS01301 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (175 a.a.); Nucleotide sequence (525 bp) |
| Description | Ectopic expression of TseTBg3 protein inhibited the growth, while co-expression of the cognate immunity (TsiTBg3) protein failed to protect the cells from effector mediated killing. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ACI79_RS22680 (TseTBg3) |
| Coordinates (Strand) | 197941..198465 (-) |
| NCBI ID | 836690956 |
| GenBank | LEKY01000067 |
| Uniprot ID | UPI00064A2E1C |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Tox-REase-5 | PF15648.8 | 6.6e-28 | 59..152 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-174 | MGGSVAAGVASIGGSTARTKDDAQAASRTIVDSRESCKSCPPATSGTKVRNNHGVNWPAYEYQARVTGFAFDTEACRWSDEWKWLGNDYDGFRPGECLLQEAKGNYDQFLDGSIPGADEFFMGFRAMMDIAAIRSRRVQANPPTRLRYYFQGPLTYQKLSAGLSKLRVESEYFP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.4890 | pos: 25-26. AQA-AS. | MGGSVAAGVASIGGSTARTKDDAQA |
Protein sequence: 175 a.a. .
>EFF01900 LEKY01000067:c198465-197941 Type VI DNase and RNase Effector (Tde) [Burkholderia gladioli]
MGGSVAAGVASIGGSTARTKDDAQAASRTIVDSRESCKSCPPATSGTKVRNNHGVNWPAYEYQARVTGFAFDTEACRWSD
EWKWLGNDYDGFRPGECLLQEAKGNYDQFLDGSIPGADEFFMGFRAMMDIAAIRSRRVQANPPTRLRYYFQGPLTYQKLS
AGLSKLRVESEYFP*
MGGSVAAGVASIGGSTARTKDDAQAASRTIVDSRESCKSCPPATSGTKVRNNHGVNWPAYEYQARVTGFAFDTEACRWSD
EWKWLGNDYDGFRPGECLLQEAKGNYDQFLDGSIPGADEFFMGFRAMMDIAAIRSRRVQANPPTRLRYYFQGPLTYQKLS
AGLSKLRVESEYFP*
Nucleotide sequence: 525 bp .
>EFF01900 LEKY01000067:c198465-197941 Type VI DNase and RNase Effector (Tde) [Burkholderia gladioli]
TTGGGCGGCTCGGTGGCGGCAGGCGTGGCTAGTATTGGGGGGAGTACGGCGCGAACGAAGGACGATGCACAAGCCGCGTC
AAGGACGATCGTCGACTCTCGGGAATCCTGCAAATCGTGTCCGCCCGCGACGAGCGGCACGAAGGTCCGAAATAATCACG
GCGTGAATTGGCCGGCCTATGAATATCAGGCACGAGTGACCGGCTTCGCCTTCGACACGGAAGCCTGCCGCTGGAGCGAC
GAGTGGAAGTGGCTGGGGAACGACTACGATGGTTTCAGGCCGGGGGAGTGCCTGCTGCAGGAGGCGAAGGGGAACTATGA
TCAATTCCTGGACGGGTCGATTCCCGGCGCGGATGAATTTTTCATGGGTTTCCGCGCAATGATGGACATTGCAGCGATCC
GCTCGCGCCGTGTGCAAGCGAACCCGCCTACGAGGCTGCGGTACTACTTTCAAGGACCTTTGACTTATCAGAAACTGTCC
GCCGGCCTGAGTAAGCTCCGTGTTGAATCTGAATACTTTCCTTGA
TTGGGCGGCTCGGTGGCGGCAGGCGTGGCTAGTATTGGGGGGAGTACGGCGCGAACGAAGGACGATGCACAAGCCGCGTC
AAGGACGATCGTCGACTCTCGGGAATCCTGCAAATCGTGTCCGCCCGCGACGAGCGGCACGAAGGTCCGAAATAATCACG
GCGTGAATTGGCCGGCCTATGAATATCAGGCACGAGTGACCGGCTTCGCCTTCGACACGGAAGCCTGCCGCTGGAGCGAC
GAGTGGAAGTGGCTGGGGAACGACTACGATGGTTTCAGGCCGGGGGAGTGCCTGCTGCAGGAGGCGAAGGGGAACTATGA
TCAATTCCTGGACGGGTCGATTCCCGGCGCGGATGAATTTTTCATGGGTTTCCGCGCAATGATGGACATTGCAGCGATCC
GCTCGCGCCGTGTGCAAGCGAACCCGCCTACGAGGCTGCGGTACTACTTTCAAGGACCTTTGACTTATCAGAAACTGTCC
GCCGGCCTGAGTAAGCTCCGTGTTGAATCTGAATACTTTCCTTGA