SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00381
Cognate effector	EFF01890
Related T6SS (Type)	T6SS01297 (Type i4b)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (99 a.a.); Nucleotide sequence (297 bp)
Description	Coexpressed TsiN was able to neutralize TseN as a defense mechanism and TsiN could inhibit TseN activity by physical binding.
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	AA098_13370 (TsiN)
Coordinates (Strand)	2938398..2938694 (-)
NCBI ID	489341217
RefSeq	NZ_CP011525
Uniprot ID	A0A2I0CH85_9PSED, A0A173HF86_9PSED, V9V059_9PSED, A0A0E9ZGI2_9PSED, A0A177YZT3_PSEPU, A0A2A3M039_9PSED
KEGG ID	pmot:X970_13170
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Tsi6	PF18660.3	3.3e-34	5..87

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-98	MKPTTPLGYVQKAIDITAQRNKACPAYPIYGMLLNQLDYVKAVFEGREQDKSKLHQLSIGAIASKEFEENDPELARALKDAYYVAIQSARGLKIQLPD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9857	-	-

Protein sequence: 99 a.a. .

                                    >IMU00381 NZ_CP011525:c2938694-2938398 [Pseudomonas sp. JY-Q]
MKPTTPLGYVQKAIDITAQRNKACPAYPIYGMLLNQLDYVKAVFEGREQDKSKLHQLSIGAIASKEFEENDPELARALKD
AYYVAIQSARGLKIQLPD*

                                

Nucleotide sequence: 297 bp .

                                    >IMU00381 NZ_CP011525:c2938694-2938398 [Pseudomonas sp. JY-Q]
ATGAAACCCACAACCCCCCTCGGCTATGTACAGAAGGCTATCGACATTACCGCGCAGCGGAACAAGGCCTGTCCCGCTTA
CCCCATTTATGGGATGCTACTAAACCAACTTGACTATGTGAAAGCCGTGTTCGAAGGGCGTGAGCAAGATAAATCGAAAC
TCCACCAACTCAGTATCGGTGCCATTGCCTCGAAAGAATTCGAAGAAAATGACCCCGAGTTGGCCAGAGCACTGAAAGAC
GCCTATTACGTAGCCATCCAGTCGGCTCGTGGCCTCAAAATTCAACTGCCCGATTGA

                                