SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01890
Effector type	Tne
Cognate immunity protein	IMU00381
Related T6SS (Type)	T6SS01297 (Type i4b)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (311 a.a.); Nucleotide sequence (933 bp)
Description	The Ntox46 domain of TseN can rapidly remove NAD1 and TseN is crucial to maintain JY-Q homeostasis and to neutralize ROS accumulation in response to stresses driven by nicotine metabolism.
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	AA098_13375 (TseN)
Coordinates (Strand)	2938691..2939623 (-)
NCBI ID	939342945
RefSeq	NZ_CP011525
Uniprot ID	UPI0006D3AF54
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Ntox46	PF15538.8	9.6e-97	146..302

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-310	MTTAWFAEDGLKPAGLDPSGGSAAQAQERVERAARQLARGQEQWVLLLLTAIVTYLTRGQMKAGVMSSMESIAARSARLQAEISNKQLAAWLAKNEQKLLAQPELQPKEATPISRAQAQPEPLRPLPDRPPIAQAPDKPGLTEVSRREYLNKKFARTGNLAQDINIRGNQETAVNFFKSQGYKPADYEGYMNGLDFTQPVFTETINRGKTLWQFQVPGGRQGMWYSPTPDVVPGQLGINPLGQIYKTETIVPKIVNVYQSTEKVTLLRSTSAPVLDTWSVPSQPYNAIGGARQMTSGQRELFKLITPGAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9892	-	-

Protein sequence: 311 a.a. .

                                    >EFF01890 NZ_CP011525:c2939623-2938691 Type VI NADase Effector (Tne) [Pseudomonas sp. JY-Q]
MTTAWFAEDGLKPAGLDPSGGSAAQAQERVERAARQLARGQEQWVLLLLTAIVTYLTRGQMKAGVMSSMESIAARSARLQ
AEISNKQLAAWLAKNEQKLLAQPELQPKEATPISRAQAQPEPLRPLPDRPPIAQAPDKPGLTEVSRREYLNKKFARTGNL
AQDINIRGNQETAVNFFKSQGYKPADYEGYMNGLDFTQPVFTETINRGKTLWQFQVPGGRQGMWYSPTPDVVPGQLGINP
LGQIYKTETIVPKIVNVYQSTEKVTLLRSTSAPVLDTWSVPSQPYNAIGGARQMTSGQRELFKLITPGAL*

                                

Nucleotide sequence: 933 bp .

                                    >EFF01890 NZ_CP011525:c2939623-2938691 Type VI NADase Effector (Tne) [Pseudomonas sp. JY-Q]
ATGACCACGGCCTGGTTCGCCGAGGATGGCCTCAAGCCCGCTGGCCTGGACCCGAGCGGCGGTTCCGCGGCGCAGGCCCA
GGAGCGTGTCGAGCGTGCTGCACGCCAGCTTGCTCGCGGGCAGGAGCAGTGGGTGTTGCTGTTACTGACAGCCATCGTCA
CCTACCTTACTCGGGGCCAGATGAAGGCCGGCGTCATGAGCAGCATGGAAAGCATTGCCGCCCGGAGCGCTCGGCTGCAG
GCCGAGATCTCCAACAAGCAGCTGGCAGCCTGGTTGGCGAAGAATGAGCAGAAACTGTTGGCGCAGCCGGAGTTGCAGCC
CAAAGAGGCCACACCGATCTCTCGTGCACAAGCGCAACCGGAGCCATTGCGACCACTACCCGATCGACCACCCATTGCTC
AGGCACCGGATAAGCCAGGCTTAACGGAAGTTTCCCGTAGGGAGTACCTGAACAAGAAGTTCGCTCGAACGGGTAACTTG
GCACAGGACATCAACATACGAGGCAACCAGGAAACTGCTGTAAACTTCTTCAAGTCACAGGGCTATAAGCCTGCCGACTA
CGAAGGCTACATGAACGGCTTGGACTTCACTCAACCAGTCTTCACGGAAACAATCAACCGGGGCAAGACACTTTGGCAAT
TCCAGGTGCCCGGCGGCCGGCAAGGCATGTGGTACTCTCCTACCCCTGACGTGGTGCCAGGGCAACTGGGGATCAACCCG
CTTGGCCAGATCTACAAAACGGAAACCATCGTGCCCAAGATCGTGAACGTCTATCAGAGCACTGAGAAGGTCACGCTGCT
GAGGAGTACCTCGGCACCGGTACTGGATACCTGGTCCGTTCCTTCCCAACCCTATAACGCCATTGGCGGTGCTCGGCAGA
TGACCTCTGGCCAACGCGAGCTCTTCAAACTGATAACACCAGGTGCATTATGA

                                