Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00379  |
| Cognate effector | EFF01855 |
| Related T6SS (Type) | T6SS01241 (Type i5) |
| Strain | Agrobacterium tumefaciens strain 12D1 |
| Replicon | chromosome |
| Sequence | Protein sequence (177 a.a.); Nucleotide sequence (531 bp) |
| Description | When induced, expression of the E4 gene led to a 40% reduction in the growth relative to E. coli carrying an empty vector and the relative growth of E. coli could be rescued by co-expressing the I4 gene. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | At12D1_RS23115 (I4) |
| Coordinates (Strand) | 1743686..1744216 (+) |
| NCBI ID | 489600127 |
| RefSeq | NZ_CP033032 |
| Uniprot ID | A0A1S7S9N0_RHIRD |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-176 | MSVTLGEAIARMQETGDWSFEEDSLWSAQDILEFEDYTSLSVPEQLAEVLVNYGWNGFEQPDVYANFVVTYEDGTRSAHEVQVLVSGKKRFYDRHDMFITSPAWPEQFTLPMIFFGTADGGNSYLLMNGRDRTDNRVWLWEQASDPFGTGNNARGLGLAAPSLYEFFYNLKKVDEI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9876 | - | - |
Protein sequence: 177 a.a. .
>IMU00379 NZ_CP033032:1743686-1744216 [Agrobacterium tumefaciens]
MSVTLGEAIARMQETGDWSFEEDSLWSAQDILEFEDYTSLSVPEQLAEVLVNYGWNGFEQPDVYANFVVTYEDGTRSAHE
VQVLVSGKKRFYDRHDMFITSPAWPEQFTLPMIFFGTADGGNSYLLMNGRDRTDNRVWLWEQASDPFGTGNNARGLGLAA
PSLYEFFYNLKKVDEI*
MSVTLGEAIARMQETGDWSFEEDSLWSAQDILEFEDYTSLSVPEQLAEVLVNYGWNGFEQPDVYANFVVTYEDGTRSAHE
VQVLVSGKKRFYDRHDMFITSPAWPEQFTLPMIFFGTADGGNSYLLMNGRDRTDNRVWLWEQASDPFGTGNNARGLGLAA
PSLYEFFYNLKKVDEI*
Nucleotide sequence: 531 bp .
>IMU00379 NZ_CP033032:1743686-1744216 [Agrobacterium tumefaciens]
ATGAGCGTTACTTTGGGTGAAGCAATCGCCAGAATGCAGGAAACTGGCGATTGGTCCTTTGAGGAAGACTCTCTCTGGTC
TGCTCAAGACATTCTTGAATTTGAGGATTACACCTCGCTGTCAGTGCCGGAGCAGCTTGCCGAGGTTCTGGTGAACTATG
GCTGGAACGGCTTCGAACAACCCGATGTCTACGCGAATTTTGTTGTGACATATGAGGATGGGACGCGCTCTGCGCATGAA
GTGCAGGTTCTGGTTTCCGGCAAGAAGCGGTTCTATGATAGACACGATATGTTCATAACCAGCCCCGCCTGGCCTGAGCA
GTTCACCCTGCCAATGATATTCTTTGGTACAGCCGACGGCGGAAACTCATATCTGCTGATGAATGGGCGGGACAGAACCG
ACAATCGTGTATGGTTGTGGGAGCAGGCTTCCGATCCTTTCGGCACGGGAAACAACGCGCGCGGCCTGGGTCTCGCAGCG
CCGTCTCTTTATGAGTTTTTCTACAATCTTAAAAAGGTCGATGAGATATAA
ATGAGCGTTACTTTGGGTGAAGCAATCGCCAGAATGCAGGAAACTGGCGATTGGTCCTTTGAGGAAGACTCTCTCTGGTC
TGCTCAAGACATTCTTGAATTTGAGGATTACACCTCGCTGTCAGTGCCGGAGCAGCTTGCCGAGGTTCTGGTGAACTATG
GCTGGAACGGCTTCGAACAACCCGATGTCTACGCGAATTTTGTTGTGACATATGAGGATGGGACGCGCTCTGCGCATGAA
GTGCAGGTTCTGGTTTCCGGCAAGAAGCGGTTCTATGATAGACACGATATGTTCATAACCAGCCCCGCCTGGCCTGAGCA
GTTCACCCTGCCAATGATATTCTTTGGTACAGCCGACGGCGGAAACTCATATCTGCTGATGAATGGGCGGGACAGAACCG
ACAATCGTGTATGGTTGTGGGAGCAGGCTTCCGATCCTTTCGGCACGGGAAACAACGCGCGCGGCCTGGGTCTCGCAGCG
CCGTCTCTTTATGAGTTTTTCTACAATCTTAAAAAGGTCGATGAGATATAA