Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00374  |
| Cognate effector | EFF01871 |
| Related T6SS (Type) | T6SS00948 (Type i1) |
| Strain | Aeromonas hydrophila NJ-35 |
| Replicon | chromosome |
| Sequence | Protein sequence (231 a.a.); Nucleotide sequence (693 bp) |
| Description | Tle1AH and Tli1Tli2AH are an effector-immunity pair |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | U876_17720 (tli2AH) |
| Coordinates (Strand) | 3862649..3863341 (-) |
| NCBI ID | 827373599 |
| GenBank | CP006870 |
| Uniprot ID | UPI000654E824 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF2931 | PF11153.10 | 2.6e-35 | 29..207 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-230 | MRHWLLLPLLLLGLAGCDDNGPTKMDWKYSVGTNADEIWHTRALFSREGGGSNESSNGVISPLSERLLKDKDYRWRLAKGRYFEGQPVPDKVEIEWVSYHDKKRYGITLELPKDLGKQMAQRYRINGDQVKQRNSIGIGLAPGGYVEVFLRRAGELPDVLVASGMAHEVTNDWYDKKVPLDNQYQERWASFDKNFRPYYDKYPIPTGEAWAPIMDAYRAAQPKTDTDPVN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9986 | pos: 16-17. LAG-CD. | MRHWLLLPLLLLGLAG |
Protein sequence: 231 a.a. .
>IMU00374 CP006870:c3863341-3862649 [Aeromonas hydrophila NJ-35]
MRHWLLLPLLLLGLAGCDDNGPTKMDWKYSVGTNADEIWHTRALFSREGGGSNESSNGVISPLSERLLKDKDYRWRLAKG
RYFEGQPVPDKVEIEWVSYHDKKRYGITLELPKDLGKQMAQRYRINGDQVKQRNSIGIGLAPGGYVEVFLRRAGELPDVL
VASGMAHEVTNDWYDKKVPLDNQYQERWASFDKNFRPYYDKYPIPTGEAWAPIMDAYRAAQPKTDTDPVN*
MRHWLLLPLLLLGLAGCDDNGPTKMDWKYSVGTNADEIWHTRALFSREGGGSNESSNGVISPLSERLLKDKDYRWRLAKG
RYFEGQPVPDKVEIEWVSYHDKKRYGITLELPKDLGKQMAQRYRINGDQVKQRNSIGIGLAPGGYVEVFLRRAGELPDVL
VASGMAHEVTNDWYDKKVPLDNQYQERWASFDKNFRPYYDKYPIPTGEAWAPIMDAYRAAQPKTDTDPVN*
Nucleotide sequence: 693 bp .
>IMU00374 CP006870:c3863341-3862649 [Aeromonas hydrophila NJ-35]
ATGAGACACTGGTTATTACTACCGTTACTGTTACTAGGGCTAGCCGGATGCGATGACAATGGCCCGACCAAGATGGATTG
GAAGTACAGCGTTGGGACTAATGCAGATGAGATTTGGCATACCCGAGCTCTGTTCTCTCGAGAGGGGGGGGGGTCTAACG
AGTCAAGTAATGGAGTTATTTCCCCGCTAAGTGAAAGGCTCTTGAAAGACAAAGATTATCGGTGGAGGTTAGCAAAAGGG
CGTTATTTTGAGGGCCAGCCAGTGCCTGACAAGGTAGAGATCGAATGGGTGTCCTATCACGATAAGAAACGGTACGGTAT
TACGCTAGAGCTCCCCAAGGATTTGGGCAAGCAGATGGCTCAGCGTTACCGTATTAATGGTGACCAAGTTAAACAGCGCA
ACAGTATAGGGATTGGATTAGCTCCGGGCGGGTATGTTGAGGTGTTTTTAAGAAGAGCTGGGGAATTACCTGACGTTCTG
GTTGCGAGTGGAATGGCGCATGAAGTGACAAACGATTGGTATGACAAGAAAGTGCCGCTAGATAACCAATATCAGGAGCG
ATGGGCCAGTTTCGATAAGAATTTCCGCCCCTATTACGACAAATATCCGATCCCAACCGGCGAGGCGTGGGCTCCTATCA
TGGATGCTTACCGCGCCGCACAGCCCAAGACGGATACAGATCCGGTCAATTGA
ATGAGACACTGGTTATTACTACCGTTACTGTTACTAGGGCTAGCCGGATGCGATGACAATGGCCCGACCAAGATGGATTG
GAAGTACAGCGTTGGGACTAATGCAGATGAGATTTGGCATACCCGAGCTCTGTTCTCTCGAGAGGGGGGGGGGTCTAACG
AGTCAAGTAATGGAGTTATTTCCCCGCTAAGTGAAAGGCTCTTGAAAGACAAAGATTATCGGTGGAGGTTAGCAAAAGGG
CGTTATTTTGAGGGCCAGCCAGTGCCTGACAAGGTAGAGATCGAATGGGTGTCCTATCACGATAAGAAACGGTACGGTAT
TACGCTAGAGCTCCCCAAGGATTTGGGCAAGCAGATGGCTCAGCGTTACCGTATTAATGGTGACCAAGTTAAACAGCGCA
ACAGTATAGGGATTGGATTAGCTCCGGGCGGGTATGTTGAGGTGTTTTTAAGAAGAGCTGGGGAATTACCTGACGTTCTG
GTTGCGAGTGGAATGGCGCATGAAGTGACAAACGATTGGTATGACAAGAAAGTGCCGCTAGATAACCAATATCAGGAGCG
ATGGGCCAGTTTCGATAAGAATTTCCGCCCCTATTACGACAAATATCCGATCCCAACCGGCGAGGCGTGGGCTCCTATCA
TGGATGCTTACCGCGCCGCACAGCCCAAGACGGATACAGATCCGGTCAATTGA