Detailed information of immunity protein


Summary

Immunity protein ID IMU00367 experimental
Cognate effector EFF01886 experimental
Related T6SS (Type) T6SS00907 experimental (Type iii)
Strain Bacteroides fragilis NCTC 9343
Replicon chromosome
Sequence Protein sequence (349 a.a.); Nucleotide sequence (1047 bp)
Description In-frame deletion of the candidate E/I pairs from the wild-type strain, and constitutive expression of the candidate immunity genes in a tssC recipient strain lacking both E/I pairs, confirmed that each immunity protein confers protection against T6S-mediated cell killing in an effector-specific manner
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) BF9343_1927 (bti2a)
Coordinates (Strand) 2352949..2353995 (-)
NCBI ID 60492932
RefSeq NC_003228
Uniprot ID UPI00004E23E5
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-34 MKGHKHSLNKYHQWADEVQVGKEAEQREERKWKK
Transmembrane helix 35-57 IFFIMALCVSLSFCAVFFVTCSL
Outside 58-66 FETATITNL
Transmembrane helix 67-89 ALSVVMMLVITSAWLIMILYLII
Inside 90-143 PKIYPKIPLINNVQLKYYGCFDFGNKWVSENIEVFKQFDDKKIIIDATSPTRNG
Transmembrane helix 144-161 AAILYIAILITLALFDFY
Outside 162-206 PKVYDNLLDGDIWGTTQAYTVYHRILDERGVLFTNNDNVMINKLS
Transmembrane helix 207-229 FASNMALASVYAIFILLALLSVL
Inside 230-348 SPRRTVIFDREKKTITIPPRWRIHKAETIPFYDAVITFGAKVDKKSFKGDEEIVIANYKHLVKGVSLQFAGRTNGYCFARLIQAYMTEDDLSNHPEFEAFKQIQEEMRINKLRKEAEKQ



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9286 - -



  Protein sequence: 349 a.a.    .

>IMU00367 NC_003228:c2353995-2352949 [Bacteroides fragilis NCTC 9343]
MKGHKHSLNKYHQWADEVQVGKEAEQREERKWKKIFFIMALCVSLSFCAVFFVTCSLFETATITNLALSVVMMLVITSAW
LIMILYLIIPKIYPKIPLINNVQLKYYGCFDFGNKWVSENIEVFKQFDDKKIIIDATSPTRNGAAILYIAILITLALFDF
YPKVYDNLLDGDIWGTTQAYTVYHRILDERGVLFTNNDNVMINKLSFASNMALASVYAIFILLALLSVLSPRRTVIFDRE
KKTITIPPRWRIHKAETIPFYDAVITFGAKVDKKSFKGDEEIVIANYKHLVKGVSLQFAGRTNGYCFARLIQAYMTEDDL
SNHPEFEAFKQIQEEMRINKLRKEAEKQ*

  Nucleotide sequence: 1047 bp    .

>IMU00367 NC_003228:c2353995-2352949 [Bacteroides fragilis NCTC 9343]
ATGAAAGGTCATAAACATTCTCTTAATAAGTATCATCAATGGGCTGATGAGGTTCAAGTAGGAAAAGAGGCAGAACAAAG
GGAAGAACGGAAATGGAAAAAGATTTTTTTCATTATGGCATTATGCGTTAGTTTATCATTTTGTGCTGTATTTTTTGTCA
CCTGTTCTCTTTTTGAAACGGCAACAATCACTAATCTTGCTCTTTCGGTAGTAATGATGTTAGTTATTACTTCTGCTTGG
CTTATTATGATACTTTATTTAATAATCCCCAAGATATATCCTAAAATTCCTCTTATCAATAACGTCCAACTTAAGTATTA
TGGTTGTTTCGATTTCGGAAATAAATGGGTTAGTGAAAATATAGAGGTATTTAAGCAGTTTGATGACAAGAAAATCATCA
TAGATGCAACCTCCCCGACTCGAAATGGTGCAGCAATTTTGTATATTGCAATTTTGATAACATTGGCATTATTTGATTTC
TATCCTAAAGTATATGACAACCTATTAGATGGAGATATCTGGGGGACTACTCAAGCTTATACTGTTTATCACAGAATTCT
GGATGAGCGAGGTGTATTATTTACAAATAATGATAACGTCATGATAAATAAACTCTCGTTTGCAAGCAATATGGCATTAG
CATCTGTTTATGCAATTTTCATTCTATTAGCACTACTTTCCGTTTTATCCCCCCGACGAACAGTTATCTTCGACCGGGAA
AAGAAAACAATCACCATTCCTCCGCGTTGGAGGATACACAAAGCGGAAACTATACCGTTTTATGATGCCGTTATTACATT
TGGTGCTAAAGTAGATAAAAAATCATTTAAGGGAGATGAGGAAATAGTAATAGCTAATTACAAACACCTCGTCAAGGGAG
TTTCTTTGCAGTTTGCTGGTCGTACAAACGGGTATTGTTTTGCTCGTTTGATTCAAGCTTATATGACTGAAGACGATTTA
TCAAATCATCCTGAATTTGAAGCTTTCAAACAGATACAAGAGGAAATGAGAATAAACAAACTACGGAAAGAGGCTGAAAA
GCAATAA