Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00367  |
| Cognate effector | EFF01886 |
| Related T6SS (Type) | T6SS00907 (Type iii) |
| Strain | Bacteroides fragilis NCTC 9343 |
| Replicon | chromosome |
| Sequence | Protein sequence (349 a.a.); Nucleotide sequence (1047 bp) |
| Description | In-frame deletion of the candidate E/I pairs from the wild-type strain, and constitutive expression of the candidate immunity genes in a tssC recipient strain lacking both E/I pairs, confirmed that each immunity protein confers protection against T6S-mediated cell killing in an effector-specific manner |
| Reference | [1] Wexler AG, Bao Y, Whitney JC, et al. Human symbionts inject and neutralize antibacterial toxins to persist in the gut. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3639-44. doi: 10.1073/pnas.1525637113. Epub 2016 Mar 8. PMID: 26957597 [2] Ross BD, Verster AJ, Radey MC, et al. Human gut bacteria contain acquired interbacterial defence systems. Nature. 2019 Nov;575(7781):224-228. doi: 10.1038/s41586-019-1708-z. Epub 2019 Oct 30. PMID: 31666699 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BF9343_1927 (bti2a) |
| Coordinates (Strand) | 2352949..2353995 (-) |
| NCBI ID | 60492932 |
| RefSeq | NC_003228 |
| Uniprot ID | UPI00004E23E5 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-34 | MKGHKHSLNKYHQWADEVQVGKEAEQREERKWKK |
| Transmembrane helix | 35-57 | IFFIMALCVSLSFCAVFFVTCSL |
| Outside | 58-66 | FETATITNL |
| Transmembrane helix | 67-89 | ALSVVMMLVITSAWLIMILYLII |
| Inside | 90-143 | PKIYPKIPLINNVQLKYYGCFDFGNKWVSENIEVFKQFDDKKIIIDATSPTRNG |
| Transmembrane helix | 144-161 | AAILYIAILITLALFDFY |
| Outside | 162-206 | PKVYDNLLDGDIWGTTQAYTVYHRILDERGVLFTNNDNVMINKLS |
| Transmembrane helix | 207-229 | FASNMALASVYAIFILLALLSVL |
| Inside | 230-348 | SPRRTVIFDREKKTITIPPRWRIHKAETIPFYDAVITFGAKVDKKSFKGDEEIVIANYKHLVKGVSLQFAGRTNGYCFARLIQAYMTEDDLSNHPEFEAFKQIQEEMRINKLRKEAEKQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9286 | - | - |
Protein sequence: 349 a.a. .
>IMU00367 NC_003228:c2353995-2352949 [Bacteroides fragilis NCTC 9343]
MKGHKHSLNKYHQWADEVQVGKEAEQREERKWKKIFFIMALCVSLSFCAVFFVTCSLFETATITNLALSVVMMLVITSAW
LIMILYLIIPKIYPKIPLINNVQLKYYGCFDFGNKWVSENIEVFKQFDDKKIIIDATSPTRNGAAILYIAILITLALFDF
YPKVYDNLLDGDIWGTTQAYTVYHRILDERGVLFTNNDNVMINKLSFASNMALASVYAIFILLALLSVLSPRRTVIFDRE
KKTITIPPRWRIHKAETIPFYDAVITFGAKVDKKSFKGDEEIVIANYKHLVKGVSLQFAGRTNGYCFARLIQAYMTEDDL
SNHPEFEAFKQIQEEMRINKLRKEAEKQ*
MKGHKHSLNKYHQWADEVQVGKEAEQREERKWKKIFFIMALCVSLSFCAVFFVTCSLFETATITNLALSVVMMLVITSAW
LIMILYLIIPKIYPKIPLINNVQLKYYGCFDFGNKWVSENIEVFKQFDDKKIIIDATSPTRNGAAILYIAILITLALFDF
YPKVYDNLLDGDIWGTTQAYTVYHRILDERGVLFTNNDNVMINKLSFASNMALASVYAIFILLALLSVLSPRRTVIFDRE
KKTITIPPRWRIHKAETIPFYDAVITFGAKVDKKSFKGDEEIVIANYKHLVKGVSLQFAGRTNGYCFARLIQAYMTEDDL
SNHPEFEAFKQIQEEMRINKLRKEAEKQ*
Nucleotide sequence: 1047 bp .
>IMU00367 NC_003228:c2353995-2352949 [Bacteroides fragilis NCTC 9343]
ATGAAAGGTCATAAACATTCTCTTAATAAGTATCATCAATGGGCTGATGAGGTTCAAGTAGGAAAAGAGGCAGAACAAAG
GGAAGAACGGAAATGGAAAAAGATTTTTTTCATTATGGCATTATGCGTTAGTTTATCATTTTGTGCTGTATTTTTTGTCA
CCTGTTCTCTTTTTGAAACGGCAACAATCACTAATCTTGCTCTTTCGGTAGTAATGATGTTAGTTATTACTTCTGCTTGG
CTTATTATGATACTTTATTTAATAATCCCCAAGATATATCCTAAAATTCCTCTTATCAATAACGTCCAACTTAAGTATTA
TGGTTGTTTCGATTTCGGAAATAAATGGGTTAGTGAAAATATAGAGGTATTTAAGCAGTTTGATGACAAGAAAATCATCA
TAGATGCAACCTCCCCGACTCGAAATGGTGCAGCAATTTTGTATATTGCAATTTTGATAACATTGGCATTATTTGATTTC
TATCCTAAAGTATATGACAACCTATTAGATGGAGATATCTGGGGGACTACTCAAGCTTATACTGTTTATCACAGAATTCT
GGATGAGCGAGGTGTATTATTTACAAATAATGATAACGTCATGATAAATAAACTCTCGTTTGCAAGCAATATGGCATTAG
CATCTGTTTATGCAATTTTCATTCTATTAGCACTACTTTCCGTTTTATCCCCCCGACGAACAGTTATCTTCGACCGGGAA
AAGAAAACAATCACCATTCCTCCGCGTTGGAGGATACACAAAGCGGAAACTATACCGTTTTATGATGCCGTTATTACATT
TGGTGCTAAAGTAGATAAAAAATCATTTAAGGGAGATGAGGAAATAGTAATAGCTAATTACAAACACCTCGTCAAGGGAG
TTTCTTTGCAGTTTGCTGGTCGTACAAACGGGTATTGTTTTGCTCGTTTGATTCAAGCTTATATGACTGAAGACGATTTA
TCAAATCATCCTGAATTTGAAGCTTTCAAACAGATACAAGAGGAAATGAGAATAAACAAACTACGGAAAGAGGCTGAAAA
GCAATAA
ATGAAAGGTCATAAACATTCTCTTAATAAGTATCATCAATGGGCTGATGAGGTTCAAGTAGGAAAAGAGGCAGAACAAAG
GGAAGAACGGAAATGGAAAAAGATTTTTTTCATTATGGCATTATGCGTTAGTTTATCATTTTGTGCTGTATTTTTTGTCA
CCTGTTCTCTTTTTGAAACGGCAACAATCACTAATCTTGCTCTTTCGGTAGTAATGATGTTAGTTATTACTTCTGCTTGG
CTTATTATGATACTTTATTTAATAATCCCCAAGATATATCCTAAAATTCCTCTTATCAATAACGTCCAACTTAAGTATTA
TGGTTGTTTCGATTTCGGAAATAAATGGGTTAGTGAAAATATAGAGGTATTTAAGCAGTTTGATGACAAGAAAATCATCA
TAGATGCAACCTCCCCGACTCGAAATGGTGCAGCAATTTTGTATATTGCAATTTTGATAACATTGGCATTATTTGATTTC
TATCCTAAAGTATATGACAACCTATTAGATGGAGATATCTGGGGGACTACTCAAGCTTATACTGTTTATCACAGAATTCT
GGATGAGCGAGGTGTATTATTTACAAATAATGATAACGTCATGATAAATAAACTCTCGTTTGCAAGCAATATGGCATTAG
CATCTGTTTATGCAATTTTCATTCTATTAGCACTACTTTCCGTTTTATCCCCCCGACGAACAGTTATCTTCGACCGGGAA
AAGAAAACAATCACCATTCCTCCGCGTTGGAGGATACACAAAGCGGAAACTATACCGTTTTATGATGCCGTTATTACATT
TGGTGCTAAAGTAGATAAAAAATCATTTAAGGGAGATGAGGAAATAGTAATAGCTAATTACAAACACCTCGTCAAGGGAG
TTTCTTTGCAGTTTGCTGGTCGTACAAACGGGTATTGTTTTGCTCGTTTGATTCAAGCTTATATGACTGAAGACGATTTA
TCAAATCATCCTGAATTTGAAGCTTTCAAACAGATACAAGAGGAAATGAGAATAAACAAACTACGGAAAGAGGCTGAAAA
GCAATAA