Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00366  |
| Cognate effector | EFF01886 |
| Related T6SS (Type) | T6SS00907 (Type iii) |
| Strain | Bacteroides fragilis NCTC 9343 |
| Replicon | chromosome |
| Sequence | Protein sequence (307 a.a.); Nucleotide sequence (921 bp) |
| Description | In-frame deletion of the candidate E/I pairs from the wild-type strain, and constitutive expression of the candidate immunity genes in a tssC recipient strain lacking both E/I pairs, confirmed that each immunity protein confers protection against T6S-mediated cell killing in an effector-specific manner |
| Reference | [1] Wexler AG, Bao Y, Whitney JC, et al. Human symbionts inject and neutralize antibacterial toxins to persist in the gut. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3639-44. doi: 10.1073/pnas.1525637113. Epub 2016 Mar 8. PMID: 26957597 [2] Ross BD, Verster AJ, Radey MC, et al. Human gut bacteria contain acquired interbacterial defence systems. Nature. 2019 Nov;575(7781):224-228. doi: 10.1038/s41586-019-1708-z. Epub 2019 Oct 30. PMID: 31666699 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BF9343_1926 (bti2b) |
| Coordinates (Strand) | 2352024..2352944 (-) |
| NCBI ID | 60492931 |
| RefSeq | NC_003228 |
| Uniprot ID | UPI00004E23E4 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-34 | MKSHKEYKNEYQRWTEEIQALNEAVQCDTKKWKI |
| Transmembrane helix | 35-57 | IGCFFALFASLSFCGSFFAACFI |
| Outside | 58-60 | CEI |
| Transmembrane helix | 61-83 | ATITNLTLSVCITILGFCFWLVL |
| Inside | 84-142 | IFYKVTPKVFPKIPLTNNDKLWRYSYFNFRDKWVYKYIDEFKQFDEKRIVIDATNPTRR |
| Transmembrane helix | 143-165 | GAPIFFLLIVIILPLLGIYSFQG |
| Outside | 166-169 | GNNN |
| Transmembrane helix | 170-192 | FAENYTIILINCVLILSGIFKFL |
| Inside | 193-306 | SPRRTIVFDREKKTITIPRRWLIHKEETIPFQKAVISFGQDALRGIDGVVIANYQHLVGEVSLQFAGRTNGYCFARLIQAYMTEDDLSNHPEFETFKQIQKEIRINKLRKKTEK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8927 | - | - |
Protein sequence: 307 a.a. .
>IMU00366 NC_003228:c2352944-2352024 [Bacteroides fragilis NCTC 9343]
MKSHKEYKNEYQRWTEEIQALNEAVQCDTKKWKIIGCFFALFASLSFCGSFFAACFICEIATITNLTLSVCITILGFCFW
LVLIFYKVTPKVFPKIPLTNNDKLWRYSYFNFRDKWVYKYIDEFKQFDEKRIVIDATNPTRRGAPIFFLLIVIILPLLGI
YSFQGGNNNFAENYTIILINCVLILSGIFKFLSPRRTIVFDREKKTITIPRRWLIHKEETIPFQKAVISFGQDALRGIDG
VVIANYQHLVGEVSLQFAGRTNGYCFARLIQAYMTEDDLSNHPEFETFKQIQKEIRINKLRKKTEK*
MKSHKEYKNEYQRWTEEIQALNEAVQCDTKKWKIIGCFFALFASLSFCGSFFAACFICEIATITNLTLSVCITILGFCFW
LVLIFYKVTPKVFPKIPLTNNDKLWRYSYFNFRDKWVYKYIDEFKQFDEKRIVIDATNPTRRGAPIFFLLIVIILPLLGI
YSFQGGNNNFAENYTIILINCVLILSGIFKFLSPRRTIVFDREKKTITIPRRWLIHKEETIPFQKAVISFGQDALRGIDG
VVIANYQHLVGEVSLQFAGRTNGYCFARLIQAYMTEDDLSNHPEFETFKQIQKEIRINKLRKKTEK*
Nucleotide sequence: 921 bp .
>IMU00366 NC_003228:c2352944-2352024 [Bacteroides fragilis NCTC 9343]
ATGAAAAGCCATAAAGAATATAAAAATGAATATCAACGTTGGACTGAGGAGATTCAAGCGTTAAATGAAGCAGTACAATG
TGATACGAAGAAATGGAAAATTATTGGATGCTTCTTTGCCTTGTTTGCAAGTCTATCATTTTGTGGCTCATTTTTTGCGG
CTTGCTTTATCTGTGAGATAGCAACAATAACTAACTTGACTTTATCAGTATGTATTACAATATTGGGATTTTGTTTTTGG
TTAGTTCTGATATTTTACAAAGTAACTCCTAAAGTTTTTCCTAAAATTCCCCTCACAAACAATGATAAACTTTGGAGGTA
TAGTTATTTTAATTTCCGAGACAAATGGGTGTATAAATACATTGATGAATTTAAACAGTTCGATGAAAAGAGAATCGTAA
TAGATGCCACCAATCCTACAAGGAGAGGAGCACCAATATTTTTCCTTCTTATCGTGATTATATTGCCTTTATTGGGCATA
TATTCTTTTCAAGGAGGAAATAATAATTTTGCTGAAAACTACACAATCATTTTGATTAATTGTGTTTTAATATTATCAGG
AATATTTAAATTCTTATCCCCCCGAAGGACAATTGTCTTTGACCGAGAAAAGAAGACAATCACTATTCCTCGCCGTTGGC
TAATCCATAAAGAAGAAACGATACCATTTCAAAAGGCGGTGATTTCCTTTGGACAGGATGCTCTCCGAGGAATTGATGGC
GTAGTCATAGCCAATTACCAGCATCTTGTTGGTGAAGTTTCTTTGCAGTTTGCTGGGCGTACAAACGGGTATTGTTTTGC
TCGTTTGATTCAAGCTTATATGACTGAAGACGATTTATCAAATCATCCTGAATTTGAAACTTTCAAGCAGATACAAAAGG
AAATAAGAATAAACAAACTACGGAAAAAGACTGAAAAGTAA
ATGAAAAGCCATAAAGAATATAAAAATGAATATCAACGTTGGACTGAGGAGATTCAAGCGTTAAATGAAGCAGTACAATG
TGATACGAAGAAATGGAAAATTATTGGATGCTTCTTTGCCTTGTTTGCAAGTCTATCATTTTGTGGCTCATTTTTTGCGG
CTTGCTTTATCTGTGAGATAGCAACAATAACTAACTTGACTTTATCAGTATGTATTACAATATTGGGATTTTGTTTTTGG
TTAGTTCTGATATTTTACAAAGTAACTCCTAAAGTTTTTCCTAAAATTCCCCTCACAAACAATGATAAACTTTGGAGGTA
TAGTTATTTTAATTTCCGAGACAAATGGGTGTATAAATACATTGATGAATTTAAACAGTTCGATGAAAAGAGAATCGTAA
TAGATGCCACCAATCCTACAAGGAGAGGAGCACCAATATTTTTCCTTCTTATCGTGATTATATTGCCTTTATTGGGCATA
TATTCTTTTCAAGGAGGAAATAATAATTTTGCTGAAAACTACACAATCATTTTGATTAATTGTGTTTTAATATTATCAGG
AATATTTAAATTCTTATCCCCCCGAAGGACAATTGTCTTTGACCGAGAAAAGAAGACAATCACTATTCCTCGCCGTTGGC
TAATCCATAAAGAAGAAACGATACCATTTCAAAAGGCGGTGATTTCCTTTGGACAGGATGCTCTCCGAGGAATTGATGGC
GTAGTCATAGCCAATTACCAGCATCTTGTTGGTGAAGTTTCTTTGCAGTTTGCTGGGCGTACAAACGGGTATTGTTTTGC
TCGTTTGATTCAAGCTTATATGACTGAAGACGATTTATCAAATCATCCTGAATTTGAAACTTTCAAGCAGATACAAAAGG
AAATAAGAATAAACAAACTACGGAAAAAGACTGAAAAGTAA