Detailed information of immunity protein


Summary

Immunity protein ID IMU00365 experimental
Cognate effector EFF01885 experimental
Related T6SS (Type) T6SS00907 experimental (Type iii)
Strain Bacteroides fragilis NCTC 9343
Replicon chromosome
Sequence Protein sequence (326 a.a.); Nucleotide sequence (978 bp)
Description In-frame deletion of the candidate E/I pairs from the wild-type strain, and constitutive expression of the candidate immunity genes in a tssC recipient strain lacking both E/I pairs, confirmed that each immunity protein confers protection against T6S-mediated cell killing in an effector-specific manner
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) BF9343_1936 (bti1)
Coordinates (Strand) 2363137..2364114 (-)
NCBI ID 60492941
RefSeq NC_003228
Uniprot ID UPI00004E23ED
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-325 MNIRNLFFLGSILLLLSCKNSKQQEGYNSINIIKYINIYNENNRILTAQGTEYDYLYFGDNKDKEILANVNNFTKTYSYDNDSSCYTVEEPLSESLLKTMRYTENTIEELVLENNKDTFSYTFSTYYDKNKPKYNKSIIILGDEPSSDSRYEEYYYYDNNGNNTKKIHHDLNTGQREETYKFNDTDYKEAVNLVPSSDYKQNIECSLKQTVNDTLITRITLNGVLNRVMKEYIDGKKKIKEELDNDMTLVNKKTEYEENGLKVNVNHTIRSTGYSTDSIYYKGNKKVKHIYNSDYNGTITLEISEYDEQGNIVKKTKKLRWPSDK



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9990 pos: 17-18. LLS-CK. MNIRNLFFLGSILLLLS



  Protein sequence: 326 a.a.    .

>IMU00365 NC_003228:c2364114-2363137 [Bacteroides fragilis NCTC 9343]
MNIRNLFFLGSILLLLSCKNSKQQEGYNSINIIKYINIYNENNRILTAQGTEYDYLYFGDNKDKEILANVNNFTKTYSYD
NDSSCYTVEEPLSESLLKTMRYTENTIEELVLENNKDTFSYTFSTYYDKNKPKYNKSIIILGDEPSSDSRYEEYYYYDNN
GNNTKKIHHDLNTGQREETYKFNDTDYKEAVNLVPSSDYKQNIECSLKQTVNDTLITRITLNGVLNRVMKEYIDGKKKIK
EELDNDMTLVNKKTEYEENGLKVNVNHTIRSTGYSTDSIYYKGNKKVKHIYNSDYNGTITLEISEYDEQGNIVKKTKKLR
WPSDK*

  Nucleotide sequence: 978 bp    .

>IMU00365 NC_003228:c2364114-2363137 [Bacteroides fragilis NCTC 9343]
ATGAATATAAGAAATTTATTTTTTTTAGGTAGTATATTACTTTTATTGAGCTGCAAAAATAGTAAACAGCAGGAAGGGTA
TAATTCTATTAATATTATTAAATATATTAATATTTATAATGAAAACAACAGGATTCTAACTGCACAAGGTACTGAATATG
ACTATCTCTATTTTGGAGACAATAAAGATAAAGAGATCTTGGCGAATGTAAATAATTTTACAAAGACATATAGTTATGAT
AATGATTCTTCCTGCTATACTGTAGAGGAACCCTTGTCGGAATCATTGCTAAAAACAATGAGATACACTGAAAATACCAT
AGAAGAACTTGTATTGGAAAATAATAAAGACACATTTAGTTATACTTTTTCTACCTATTATGATAAGAATAAGCCTAAGT
ATAATAAAAGTATTATAATATTAGGAGATGAACCCTCTTCTGACTCAAGATATGAGGAATACTACTACTATGATAATAAT
GGAAATAATACGAAGAAAATCCATCATGATTTAAACACCGGGCAAAGAGAAGAAACCTATAAATTTAATGATACAGATTA
TAAAGAAGCTGTTAATCTTGTCCCTTCTTCCGATTACAAACAAAATATCGAGTGTTCATTGAAACAAACTGTTAATGATA
CCTTAATCACTCGGATTACCTTAAACGGTGTACTTAATAGAGTGATGAAGGAATACATTGATGGAAAAAAGAAAATTAAG
GAAGAGTTGGACAATGATATGACTTTAGTCAATAAAAAAACAGAGTATGAAGAAAACGGACTGAAAGTAAACGTCAATCA
TACTATAAGAAGTACAGGCTACTCAACCGACAGTATCTATTATAAAGGGAACAAAAAAGTTAAGCATATCTACAATTCCG
ACTACAATGGTACCATAACACTTGAAATCTCAGAATATGATGAGCAAGGAAATATAGTAAAGAAAACAAAAAAACTCAGA
TGGCCATCAGATAAATAA