Detailed information of immunity protein
Summary
Immunity protein ID | IMU00365 |
Cognate effector | EFF01885 |
Related T6SS (Type) | T6SS00907 (Type iii) |
Strain | Bacteroides fragilis NCTC 9343 |
Replicon | chromosome |
Sequence | Protein sequence (326 a.a.); Nucleotide sequence (978 bp) |
Description | In-frame deletion of the candidate E/I pairs from the wild-type strain, and constitutive expression of the candidate immunity genes in a tssC recipient strain lacking both E/I pairs, confirmed that each immunity protein confers protection against T6S-mediated cell killing in an effector-specific manner |
Reference | [1] Wexler AG, Bao Y, Whitney JC, et al. Human symbionts inject and neutralize antibacterial toxins to persist in the gut. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3639-44. doi: 10.1073/pnas.1525637113. Epub 2016 Mar 8. PMID: 26957597 [2] Ross BD, Verster AJ, Radey MC, et al. Human gut bacteria contain acquired interbacterial defence systems. Nature. 2019 Nov;575(7781):224-228. doi: 10.1038/s41586-019-1708-z. Epub 2019 Oct 30. PMID: 31666699 |
Bioinformatics investigation has been performed
External database links
Locus tag (Gene) | BF9343_1936 (bti1) |
Coordinates (Strand) | 2363137..2364114 (-) |
NCBI ID | 60492941 |
RefSeq | NC_003228 |
Uniprot ID | UPI00004E23ED |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Outside | 1-325 | MNIRNLFFLGSILLLLSCKNSKQQEGYNSINIIKYINIYNENNRILTAQGTEYDYLYFGDNKDKEILANVNNFTKTYSYDNDSSCYTVEEPLSESLLKTMRYTENTIEELVLENNKDTFSYTFSTYYDKNKPKYNKSIIILGDEPSSDSRYEEYYYYDNNGNNTKKIHHDLNTGQREETYKFNDTDYKEAVNLVPSSDYKQNIECSLKQTVNDTLITRITLNGVLNRVMKEYIDGKKKIKEELDNDMTLVNKKTEYEENGLKVNVNHTIRSTGYSTDSIYYKGNKKVKHIYNSDYNGTITLEISEYDEQGNIVKKTKKLRWPSDK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
LIPO(Sec/SPII) | 0.9990 | pos: 17-18. LLS-CK. | MNIRNLFFLGSILLLLS |
Protein sequence: 326 a.a. .
>IMU00365 NC_003228:c2364114-2363137 [Bacteroides fragilis NCTC 9343]
MNIRNLFFLGSILLLLSCKNSKQQEGYNSINIIKYINIYNENNRILTAQGTEYDYLYFGDNKDKEILANVNNFTKTYSYD
NDSSCYTVEEPLSESLLKTMRYTENTIEELVLENNKDTFSYTFSTYYDKNKPKYNKSIIILGDEPSSDSRYEEYYYYDNN
GNNTKKIHHDLNTGQREETYKFNDTDYKEAVNLVPSSDYKQNIECSLKQTVNDTLITRITLNGVLNRVMKEYIDGKKKIK
EELDNDMTLVNKKTEYEENGLKVNVNHTIRSTGYSTDSIYYKGNKKVKHIYNSDYNGTITLEISEYDEQGNIVKKTKKLR
WPSDK*
MNIRNLFFLGSILLLLSCKNSKQQEGYNSINIIKYINIYNENNRILTAQGTEYDYLYFGDNKDKEILANVNNFTKTYSYD
NDSSCYTVEEPLSESLLKTMRYTENTIEELVLENNKDTFSYTFSTYYDKNKPKYNKSIIILGDEPSSDSRYEEYYYYDNN
GNNTKKIHHDLNTGQREETYKFNDTDYKEAVNLVPSSDYKQNIECSLKQTVNDTLITRITLNGVLNRVMKEYIDGKKKIK
EELDNDMTLVNKKTEYEENGLKVNVNHTIRSTGYSTDSIYYKGNKKVKHIYNSDYNGTITLEISEYDEQGNIVKKTKKLR
WPSDK*
Nucleotide sequence: 978 bp .
>IMU00365 NC_003228:c2364114-2363137 [Bacteroides fragilis NCTC 9343]
ATGAATATAAGAAATTTATTTTTTTTAGGTAGTATATTACTTTTATTGAGCTGCAAAAATAGTAAACAGCAGGAAGGGTA
TAATTCTATTAATATTATTAAATATATTAATATTTATAATGAAAACAACAGGATTCTAACTGCACAAGGTACTGAATATG
ACTATCTCTATTTTGGAGACAATAAAGATAAAGAGATCTTGGCGAATGTAAATAATTTTACAAAGACATATAGTTATGAT
AATGATTCTTCCTGCTATACTGTAGAGGAACCCTTGTCGGAATCATTGCTAAAAACAATGAGATACACTGAAAATACCAT
AGAAGAACTTGTATTGGAAAATAATAAAGACACATTTAGTTATACTTTTTCTACCTATTATGATAAGAATAAGCCTAAGT
ATAATAAAAGTATTATAATATTAGGAGATGAACCCTCTTCTGACTCAAGATATGAGGAATACTACTACTATGATAATAAT
GGAAATAATACGAAGAAAATCCATCATGATTTAAACACCGGGCAAAGAGAAGAAACCTATAAATTTAATGATACAGATTA
TAAAGAAGCTGTTAATCTTGTCCCTTCTTCCGATTACAAACAAAATATCGAGTGTTCATTGAAACAAACTGTTAATGATA
CCTTAATCACTCGGATTACCTTAAACGGTGTACTTAATAGAGTGATGAAGGAATACATTGATGGAAAAAAGAAAATTAAG
GAAGAGTTGGACAATGATATGACTTTAGTCAATAAAAAAACAGAGTATGAAGAAAACGGACTGAAAGTAAACGTCAATCA
TACTATAAGAAGTACAGGCTACTCAACCGACAGTATCTATTATAAAGGGAACAAAAAAGTTAAGCATATCTACAATTCCG
ACTACAATGGTACCATAACACTTGAAATCTCAGAATATGATGAGCAAGGAAATATAGTAAAGAAAACAAAAAAACTCAGA
TGGCCATCAGATAAATAA
ATGAATATAAGAAATTTATTTTTTTTAGGTAGTATATTACTTTTATTGAGCTGCAAAAATAGTAAACAGCAGGAAGGGTA
TAATTCTATTAATATTATTAAATATATTAATATTTATAATGAAAACAACAGGATTCTAACTGCACAAGGTACTGAATATG
ACTATCTCTATTTTGGAGACAATAAAGATAAAGAGATCTTGGCGAATGTAAATAATTTTACAAAGACATATAGTTATGAT
AATGATTCTTCCTGCTATACTGTAGAGGAACCCTTGTCGGAATCATTGCTAAAAACAATGAGATACACTGAAAATACCAT
AGAAGAACTTGTATTGGAAAATAATAAAGACACATTTAGTTATACTTTTTCTACCTATTATGATAAGAATAAGCCTAAGT
ATAATAAAAGTATTATAATATTAGGAGATGAACCCTCTTCTGACTCAAGATATGAGGAATACTACTACTATGATAATAAT
GGAAATAATACGAAGAAAATCCATCATGATTTAAACACCGGGCAAAGAGAAGAAACCTATAAATTTAATGATACAGATTA
TAAAGAAGCTGTTAATCTTGTCCCTTCTTCCGATTACAAACAAAATATCGAGTGTTCATTGAAACAAACTGTTAATGATA
CCTTAATCACTCGGATTACCTTAAACGGTGTACTTAATAGAGTGATGAAGGAATACATTGATGGAAAAAAGAAAATTAAG
GAAGAGTTGGACAATGATATGACTTTAGTCAATAAAAAAACAGAGTATGAAGAAAACGGACTGAAAGTAAACGTCAATCA
TACTATAAGAAGTACAGGCTACTCAACCGACAGTATCTATTATAAAGGGAACAAAAAAGTTAAGCATATCTACAATTCCG
ACTACAATGGTACCATAACACTTGAAATCTCAGAATATGATGAGCAAGGAAATATAGTAAAGAAAACAAAAAAACTCAGA
TGGCCATCAGATAAATAA