Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00353  |
| Cognate effector | EFF01834 |
| Related T6SS (Type) | T6SS01247 (Type i1) |
| Strain | Vibrio parahaemolyticus strain T9109 |
| Replicon | chromosome |
| Sequence | Protein sequence (152 a.a.); Nucleotide sequence (456 bp) |
| Description | The Δtme/i2 mutant was no longer able to antagonize T6SS1-mediated attacks due to loss of Tmi2-mediated immunity |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PO79_RS05915 (Tmi2) |
| Coordinates (Strand) | 166529..166984 (+) |
| NCBI ID | 646368409 |
| RefSeq | NZ_JTGR01000043 |
| Uniprot ID | UPI000472734B |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-12 | MSDSQLINKIKR |
| Transmembrane helix | 13-35 | VSKFIPATIIGAGLTIACFYLGL |
| Outside | 36-54 | GKIESFSQEIGNVEVYSRF |
| Transmembrane helix | 55-77 | DFSLIPFGIVMLGFTITAVYYIY |
| Inside | 78-88 | YEKRFEDAFGH |
| Transmembrane helix | 89-111 | ILNYTMLGAFALSIILVFITPSI |
| Outside | 112-151 | YQNRFENAGLKECNGTPSSYLPLFGTKFAKDESLCRIRNH |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8455 | - | - |
Protein sequence: 152 a.a. .
>IMU00353 NZ_JTGR01000043:166529-166984 [Vibrio parahaemolyticus]
MSDSQLINKIKRVSKFIPATIIGAGLTIACFYLGLGKIESFSQEIGNVEVYSRFDFSLIPFGIVMLGFTITAVYYIYYEK
RFEDAFGHILNYTMLGAFALSIILVFITPSIYQNRFENAGLKECNGTPSSYLPLFGTKFAKDESLCRIRNH*
MSDSQLINKIKRVSKFIPATIIGAGLTIACFYLGLGKIESFSQEIGNVEVYSRFDFSLIPFGIVMLGFTITAVYYIYYEK
RFEDAFGHILNYTMLGAFALSIILVFITPSIYQNRFENAGLKECNGTPSSYLPLFGTKFAKDESLCRIRNH*
Nucleotide sequence: 456 bp .
>IMU00353 NZ_JTGR01000043:166529-166984 [Vibrio parahaemolyticus]
ATGAGCGACTCCCAATTGATAAACAAAATTAAAAGAGTCTCAAAATTTATTCCAGCAACAATCATTGGAGCTGGATTGAC
TATCGCCTGTTTTTATTTAGGCTTGGGCAAGATAGAAAGCTTCAGCCAAGAGATCGGTAATGTAGAGGTATATTCTCGCT
TTGATTTTAGCCTCATACCATTCGGTATCGTTATGCTCGGATTTACCATTACGGCTGTATATTATATTTACTATGAAAAA
CGGTTTGAAGACGCATTCGGTCACATTCTGAATTACACAATGTTAGGAGCATTTGCCCTTTCAATAATCTTAGTTTTTAT
AACGCCATCTATCTATCAAAACCGCTTTGAAAATGCTGGACTAAAAGAGTGCAATGGTACACCAAGCAGCTATCTGCCGC
TATTTGGTACAAAGTTCGCCAAAGACGAATCTTTATGTCGAATAAGGAATCACTAG
ATGAGCGACTCCCAATTGATAAACAAAATTAAAAGAGTCTCAAAATTTATTCCAGCAACAATCATTGGAGCTGGATTGAC
TATCGCCTGTTTTTATTTAGGCTTGGGCAAGATAGAAAGCTTCAGCCAAGAGATCGGTAATGTAGAGGTATATTCTCGCT
TTGATTTTAGCCTCATACCATTCGGTATCGTTATGCTCGGATTTACCATTACGGCTGTATATTATATTTACTATGAAAAA
CGGTTTGAAGACGCATTCGGTCACATTCTGAATTACACAATGTTAGGAGCATTTGCCCTTTCAATAATCTTAGTTTTTAT
AACGCCATCTATCTATCAAAACCGCTTTGAAAATGCTGGACTAAAAGAGTGCAATGGTACACCAAGCAGCTATCTGCCGC
TATTTGGTACAAAGTTCGCCAAAGACGAATCTTTATGTCGAATAAGGAATCACTAG