SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00347
Cognate effector	EFF01811
Related T6SS (Type)	T6SS01063 (Type i3)
Strain	Bradyrhizobium japonicum strain J5
Replicon	chromosome
Sequence	Protein sequence (308 a.a.); Nucleotide sequence (924 bp)
Description	-
Reference	[1] Burkinshaw BJ, Liang X, Wong M, et al. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol. 2018 May;3(5):632-640. doi: 10.1038/s41564-018-0144-4. Epub 2018 Apr 9. PMID: 29632369

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	RS38465 (TsiT)
Coordinates (Strand)	8075210..8076133 (+)
NCBI ID	1110689930
RefSeq	NZ_CP017637
Uniprot ID	A0A1L3FLS5_BRAJP
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
HTH_1	PF00126.29	9.6e-17	8..67
LysR_substrate	PF03466.22	1.1e-16	94..284

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-307	MNLNSLDLNLLTALDALLREANVSRAALRIGLSQPAASHALQRLRDIFGDPLLVRTGARMELTPRAQALRAPLAQALDQVRGLFIPDDFDAARSERQFRLMMPDLAVELLMPPLMEKVTRAAPNVRIDVVPWRGPAIFHAEFARTIDLVISIGNAFKGFHRQLLYTDSDALAVRRGHPMAAKLKRRETFLAARHVGVIIRGQPEDLIDTWLRAKGIERHISLVVSGYLEALHVAARTDLVAFVPRRLIAALSKQLGLVAVTPPLDPGIDEQFMFHPTRAQMDPGSIWLRRLMLATGRELEKKERRAG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9540	-	-

Protein sequence: 308 a.a. .

                                    >IMU00347 NZ_CP017637:8075210-8076133 [Bradyrhizobium japonicum]
MNLNSLDLNLLTALDALLREANVSRAALRIGLSQPAASHALQRLRDIFGDPLLVRTGARMELTPRAQALRAPLAQALDQV
RGLFIPDDFDAARSERQFRLMMPDLAVELLMPPLMEKVTRAAPNVRIDVVPWRGPAIFHAEFARTIDLVISIGNAFKGFH
RQLLYTDSDALAVRRGHPMAAKLKRRETFLAARHVGVIIRGQPEDLIDTWLRAKGIERHISLVVSGYLEALHVAARTDLV
AFVPRRLIAALSKQLGLVAVTPPLDPGIDEQFMFHPTRAQMDPGSIWLRRLMLATGRELEKKERRAG*

                                

Nucleotide sequence: 924 bp .

                                    >IMU00347 NZ_CP017637:8075210-8076133 [Bradyrhizobium japonicum]
ATGAATTTGAATTCGCTTGACCTCAATCTCCTGACCGCGCTCGACGCGCTGCTGCGCGAGGCCAATGTCAGCCGGGCCGC
GCTTCGGATCGGGTTGTCGCAGCCGGCCGCGAGCCATGCGTTGCAACGCCTGCGCGACATCTTCGGCGATCCGCTGCTGG
TGCGCACCGGCGCGCGCATGGAGCTGACGCCGCGGGCGCAAGCGCTCCGCGCGCCGCTGGCCCAGGCGCTCGACCAGGTG
CGCGGGCTGTTCATACCCGACGACTTCGATGCCGCGCGCAGCGAGCGGCAATTCCGCCTGATGATGCCGGATCTCGCGGT
CGAGCTGCTGATGCCGCCCTTGATGGAGAAGGTGACGCGGGCCGCGCCCAACGTCCGCATCGACGTCGTACCGTGGCGGG
GCCCTGCGATCTTTCACGCCGAGTTCGCCCGCACCATCGACCTCGTGATCTCGATCGGCAACGCCTTCAAGGGCTTTCAC
CGCCAGTTGCTCTACACCGACAGCGACGCACTGGCGGTGCGGCGCGGCCATCCCATGGCTGCGAAGCTGAAGCGGCGCGA
GACGTTCCTGGCAGCGCGCCATGTCGGCGTGATCATCCGCGGCCAACCGGAGGATCTCATCGACACCTGGCTGCGAGCCA
AGGGCATCGAGCGGCACATTTCGCTGGTGGTGTCAGGCTATCTCGAAGCGCTGCACGTCGCCGCCCGCACCGACCTCGTC
GCCTTCGTGCCGCGCCGGCTGATCGCGGCGCTGTCGAAGCAGCTCGGCCTCGTCGCGGTGACGCCGCCGCTCGATCCCGG
GATCGACGAGCAGTTCATGTTCCATCCGACCCGCGCACAGATGGACCCGGGCTCGATCTGGCTGCGGCGGCTGATGCTGG
CGACGGGACGGGAATTGGAGAAGAAGGAACGGCGTGCTGGGTAG

                                