SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01811
Effector type	Tde
Cognate immunity protein	IMU00347
Related T6SS (Type)	T6SS01063 (Type i3)
Strain	Bradyrhizobium japonicum strain J5
Replicon	chromosome
Sequence	Protein sequence (325 a.a.); Nucleotide sequence (975 bp)
Description	TOX-REase-5
Reference	[1] Burkinshaw BJ, Liang X, Wong M, et al. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol. 2018 May;3(5):632-640. doi: 10.1038/s41564-018-0144-4. Epub 2018 Apr 9. PMID: 29632369

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	RS15455 (TseT)
Coordinates (Strand)	3215663..3216637 (-)
NCBI ID	1173687256
RefSeq	NZ_CP017637
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Usp	PF00582.28	1.1e-11	201..323

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-324	MTVNPTVVFDSSTDNPHLVQMLSLRLWAQLGLTTIMASAGRSAVFGCLINKGWPNYWERTAMIKDILVRLDGTPQDDIRLAAVDQIATIFESHITGLYFNLQSPLTSGEAEREDGDGVEAAALARLRRLQQETHLRRFDVAGEEDVPDTAMLPARAADTFVELCPDSRSSNPERLIERLLFGAGRHVFLVPNDLKAVSFWKHAIVAWNGSREAARALAEAMPYLHLAEKVGLLVVEGEHPTEADLLMGNDAVQHLRHHGINAVKYRAFGEEDETAGVLIEECRKLDANLLVMGSYGHSTLHELLPGSTTSRLLRRSPIPLVVAH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8896	-	-

Protein sequence: 325 a.a. .

                                    >EFF01811 NZ_CP017637:c3216637-3215663 Type VI DNase and RNase Effector (Tde) [Bradyrhizobium japonicum]
MTVNPTVVFDSSTDNPHLVQMLSLRLWAQLGLTTIMASAGRSAVFGCLINKGWPNYWERTAMIKDILVRLDGTPQDDIRL
AAVDQIATIFESHITGLYFNLQSPLTSGEAEREDGDGVEAAALARLRRLQQETHLRRFDVAGEEDVPDTAMLPARAADTF
VELCPDSRSSNPERLIERLLFGAGRHVFLVPNDLKAVSFWKHAIVAWNGSREAARALAEAMPYLHLAEKVGLLVVEGEHP
TEADLLMGNDAVQHLRHHGINAVKYRAFGEEDETAGVLIEECRKLDANLLVMGSYGHSTLHELLPGSTTSRLLRRSPIPL
VVAH*

                                

Nucleotide sequence: 975 bp .

                                    >EFF01811 NZ_CP017637:c3216637-3215663 Type VI DNase and RNase Effector (Tde) [Bradyrhizobium japonicum]
ATGACCGTCAACCCGACCGTGGTTTTCGATAGCTCTACCGATAATCCGCACCTCGTCCAAATGCTGAGCCTGAGACTTTG
GGCCCAGCTTGGCTTGACAACCATCATGGCATCTGCCGGCCGCTCCGCGGTATTTGGCTGCTTGATCAATAAAGGCTGGC
CGAACTACTGGGAAAGGACTGCCATGATCAAGGACATACTCGTTCGTCTCGACGGAACGCCGCAGGACGACATTCGTCTG
GCGGCCGTCGACCAGATTGCCACGATATTTGAATCTCACATCACCGGTCTCTACTTCAATCTGCAGTCACCTCTCACCTC
CGGCGAAGCCGAAAGGGAGGACGGCGACGGGGTCGAAGCAGCCGCGCTGGCTCGTTTGAGACGCCTTCAGCAGGAGACGC
ACCTGCGTCGCTTTGATGTCGCGGGCGAAGAGGACGTCCCTGATACGGCCATGCTGCCAGCGCGCGCCGCCGACACGTTC
GTGGAGCTCTGTCCCGACAGCCGATCGAGCAACCCGGAGCGCTTGATCGAGCGCCTCCTTTTCGGAGCCGGCCGTCACGT
ATTCCTCGTGCCGAACGACCTCAAAGCAGTTTCTTTCTGGAAGCACGCAATCGTCGCATGGAACGGCAGTCGGGAGGCGG
CCCGCGCCTTGGCCGAGGCCATGCCTTATTTGCATCTGGCCGAGAAGGTCGGCCTGCTCGTCGTCGAAGGCGAGCACCCG
ACGGAGGCAGACCTCCTCATGGGAAACGATGCCGTACAACATCTTCGGCATCACGGCATCAATGCCGTCAAATACCGGGC
GTTTGGTGAGGAAGACGAGACCGCCGGCGTGCTGATCGAGGAATGTCGCAAGCTGGACGCCAATCTGCTGGTGATGGGCA
GTTACGGCCATTCCACCCTGCATGAATTGCTACCCGGCAGTACGACGAGCCGCCTGCTTCGTCGTTCGCCGATCCCGCTC
GTCGTTGCCCATTGA

                                