SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00346
Cognate effector	EFF01804
Related T6SS (Type)	T6SS01046 (Type i4b)
Strain	Acinetobacter baumannii strain ATCC 17978
Replicon	chromosome
Sequence	Protein sequence (287 a.a.); Nucleotide sequence (861 bp)
Description	Expression of Tdi in E. coli was protective against killing by the ΔvgrG34 strain, confirming Tde and Tdi as an effector-immunity pair.
Reference	[1] Weber BS, Hennon SW, Wright MS, et al. Genetic Dissection of the Type VI Secretion System in Acinetobacter and Identification of a Novel Peptidoglycan Hydrolase, TagX, Required for Its Biogenesis. mBio. 2016 Oct 11;7(5). pii: e01253-16. doi: 10.1128/mBio.01253-16. PMID: 27729508

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ACX60_15360 (Tdi)
Coordinates (Strand)	3245298..3246158 (-)
NCBI ID	884901468
RefSeq	NZ_CP012004
Uniprot ID	A0A1E3M7D7_ACIBA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

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Domain	Pfam ID	E-value	Aligned region
GAD-like	PF08887.13	2.3e-27	6..99
DUF1851	PF08906.13	4.6e-21	129..198

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Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction	Region	Sequence
Outside	1-286	MNQEDIDYFYEKYGQPIDKVEVTEDIIKKYRGKLPESILEQWHLFGFAGYLNGLYWITNPDDYAEVIYDWLEETPLPDDDVYHVLARSAFGELLIWGERNYGRYYIKTMEGILHDNGEQLESAEFYGSDFFFLPKKNYLDYTDKNGNKLFDRAVKKLGVLKADEMYAFEPALALGGEESLQHLTKVNLPVHMKLLKQVTPLRLRTFEDLTAALYGTSYSVDDLTSGQDAESQYQESVQAGEVCPRTGYWTTPAQPNTRHYCKKGEVLPEIKEQDWGEVYWYWDGEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

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Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9976	-	-

Protein sequence: 287 a.a. .

                                    >IMU00346 NZ_CP012004:c3246158-3245298 [Acinetobacter baumannii]
MNQEDIDYFYEKYGQPIDKVEVTEDIIKKYRGKLPESILEQWHLFGFAGYLNGLYWITNPDDYAEVIYDWLEETPLPDDD
VYHVLARSAFGELLIWGERNYGRYYIKTMEGILHDNGEQLESAEFYGSDFFFLPKKNYLDYTDKNGNKLFDRAVKKLGVL
KADEMYAFEPALALGGEESLQHLTKVNLPVHMKLLKQVTPLRLRTFEDLTAALYGTSYSVDDLTSGQDAESQYQESVQAG
EVCPRTGYWTTPAQPNTRHYCKKGEVLPEIKEQDWGEVYWYWDGEN*

                                

Nucleotide sequence: 861 bp .

                                    >IMU00346 NZ_CP012004:c3246158-3245298 [Acinetobacter baumannii]
ATGAACCAAGAAGATATTGATTACTTTTATGAGAAATATGGTCAACCAATTGATAAAGTTGAGGTGACAGAGGACATCAT
AAAAAAATATCGGGGTAAACTGCCTGAAAGTATTTTAGAACAATGGCATTTATTTGGTTTTGCTGGTTATTTGAATGGTT
TGTACTGGATTACGAACCCAGATGATTATGCTGAAGTAATTTATGACTGGTTAGAGGAGACACCATTGCCTGATGATGAT
GTTTATCATGTATTGGCCCGTTCCGCATTTGGTGAGCTATTAATTTGGGGTGAACGTAATTATGGTAGATATTACATTAA
AACAATGGAAGGTATCCTACATGACAATGGCGAACAGCTAGAAAGCGCTGAGTTTTATGGTAGTGATTTTTTCTTTTTAC
CTAAAAAAAATTATCTAGATTACACAGACAAAAATGGCAATAAACTATTTGATCGTGCTGTAAAAAAATTAGGTGTTTTA
AAGGCAGATGAAATGTATGCATTTGAACCTGCTTTGGCATTAGGTGGGGAAGAATCATTACAGCACCTAACAAAAGTTAA
TCTTCCTGTTCACATGAAGCTACTAAAACAGGTAACTCCATTAAGATTGAGAACTTTTGAGGATCTTACGGCTGCTCTAT
ATGGTACATCGTATAGTGTGGACGATTTAACAAGTGGACAAGATGCTGAGTCACAATATCAAGAATCTGTTCAAGCTGGA
GAAGTTTGTCCTAGAACAGGTTACTGGACAACGCCTGCACAACCCAATACACGCCATTATTGTAAGAAAGGTGAAGTTCT
TCCTGAAATTAAGGAACAAGACTGGGGTGAAGTGTATTGGTATTGGGATGGTGAGAACTGA

                                