Detailed information of immunity protein


Summary

Immunity protein ID IMU00331 experimental
Cognate effector EFF01495 experimental
Related T6SS (Type) T6SS00252 insolico (Type i4b)
Strain Pseudomonas putida GB-1
Replicon chromosome
Sequence Protein sequence (372 a.a.); Nucleotide sequence (1116 bp)
Description ARH activity of Tri1-Pp is specific and that its capacity to neutralize Tre1-Sp derives from the substrate overlap between this protein and its own cognate effector, Tre1-Pp
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) PPUTGB1_RS14045 (Tri1)
Coordinates (Strand) 3096203..3097318 (-)
NCBI ID 501229376
RefSeq NC_010322
Uniprot ID TRI1_PSEPG
KEGG ID ppg:PputGB1_2761
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ADP_ribosyl_GH PF03747.16 1.6e-59 70..334
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-371 MIDLRSPNALLSDYVERYAHLSPEPSRQLQQRMDYNVRADAPAEPASKPRWLQSRACTLTPEQALDRAKGALLGLAIGDAVGTTLEFLPRDREHVNDMVGGGPFRLAAGEWTDDTSMALCLADTYVSQGKFDYATYANALVRWYRHGENSVNGRCFDIGNATRNALEGWLREGIGWQGNYDPSTAGNGSIIRLAPTAIFRRHSLSASWWESVTQSSVTHNADEAVNCCQLLAAQLHLALNGADKEETLAPAVRSLRPRPMIINAGEYKQKSRDQIRSSGYVVDTLEAALWAVWNSNNFHDAILLAANLADDADSVAATAGQLAGALYGVSGMPPEWVEKVAWSQHIQKLAQELFDRAPQVDELDALLYGKR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9840 - -


  Protein sequence: 372 a.a.    .

>IMU00331 NC_010322:c3097318-3096203 [Pseudomonas putida GB-1]
MIDLRSPNALLSDYVERYAHLSPEPSRQLQQRMDYNVRADAPAEPASKPRWLQSRACTLTPEQALDRAKGALLGLAIGDA
VGTTLEFLPRDREHVNDMVGGGPFRLAAGEWTDDTSMALCLADTYVSQGKFDYATYANALVRWYRHGENSVNGRCFDIGN
ATRNALEGWLREGIGWQGNYDPSTAGNGSIIRLAPTAIFRRHSLSASWWESVTQSSVTHNADEAVNCCQLLAAQLHLALN
GADKEETLAPAVRSLRPRPMIINAGEYKQKSRDQIRSSGYVVDTLEAALWAVWNSNNFHDAILLAANLADDADSVAATAG
QLAGALYGVSGMPPEWVEKVAWSQHIQKLAQELFDRAPQVDELDALLYGKR*

  Nucleotide sequence: 1116 bp    .

>IMU00331 NC_010322:c3097318-3096203 [Pseudomonas putida GB-1]
ATGATCGATCTAAGAAGCCCAAACGCATTGCTGTCCGACTACGTCGAGCGCTACGCGCACCTGTCGCCGGAGCCCTCCCG
GCAGCTGCAGCAGAGGATGGACTACAACGTGCGGGCAGACGCCCCAGCAGAGCCGGCGAGCAAGCCGCGCTGGTTGCAGT
CCAGGGCCTGTACCTTGACGCCAGAACAGGCACTGGACCGCGCCAAAGGCGCGTTGCTGGGCCTGGCCATTGGCGATGCG
GTAGGCACCACGCTCGAGTTCCTGCCGCGCGACCGGGAGCATGTGAACGACATGGTCGGTGGCGGGCCATTCAGGCTTGC
TGCCGGCGAGTGGACCGATGACACTAGCATGGCGCTTTGCTTGGCGGATACGTATGTAAGCCAGGGCAAGTTCGACTACG
CCACCTACGCAAATGCCTTGGTGCGCTGGTATCGACACGGGGAAAACAGTGTCAATGGCCGTTGTTTCGACATCGGCAAC
GCTACACGTAACGCGCTTGAAGGCTGGTTGCGCGAAGGCATCGGTTGGCAAGGCAACTACGACCCGTCCACTGCAGGTAA
TGGCTCGATCATACGCCTCGCGCCCACGGCCATCTTTCGTCGACATTCACTGTCTGCGAGTTGGTGGGAAAGCGTCACGC
AGAGCAGCGTGACCCATAACGCCGATGAAGCGGTGAACTGTTGCCAGTTGCTCGCCGCACAACTGCATCTGGCACTGAAC
GGTGCAGACAAGGAAGAAACCCTTGCACCTGCGGTGCGCTCGCTGCGTCCGCGCCCGATGATCATCAATGCTGGCGAGTA
CAAACAGAAGAGCCGCGATCAGATCCGCTCTTCTGGTTATGTGGTCGACACGCTGGAGGCAGCCCTGTGGGCTGTATGGA
ACAGCAACAACTTCCATGACGCGATTCTTCTGGCCGCCAACCTTGCCGATGATGCTGACAGCGTCGCGGCCACAGCCGGC
CAGCTGGCTGGCGCGCTGTACGGTGTGTCAGGCATGCCACCGGAGTGGGTGGAGAAGGTCGCCTGGTCGCAGCATATCCA
GAAGCTGGCACAGGAACTGTTCGACCGCGCACCGCAGGTGGATGAACTGGATGCACTGTTGTACGGGAAGCGATAA