SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00305
Cognate effector	EFF01484
Related T6SS (Type)	T6SS00312 (Type i4b)
Strain	Acinetobacter baumannii AB307-0294
Replicon	chromosome
Sequence	Protein sequence (288 a.a.); Nucleotide sequence (864 bp)
Description	Expression of rhs1-CT alone within the cytoplasm of E. coli resulted in the arrest of E. coli growth within 1 h following induction of expression. E. coli expressing both rhs1-CT and the predicted cognate immunity gene, rhs1I, grew at normal WT levels, confirming that Rhs1 and Rhs1I are a genuine effector-immunity pair.
Reference	[1] Fitzsimons TC, Lewis JM, Wright A, et al. Identification of Novel Acinetobacter baumannii Type VI Secretion System Antibacterial Effector and Immunity Pairs. Infect Immun. 2018 Jul 23;86(8). pii: e00297-18. doi: 10.1128/IAI.00297-18. Print 2018 Aug. PMID: 29735524

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ABBFA_02467 (Rhs1I)
Coordinates (Strand)	2669468..2670331 (-)
NCBI ID	446971646
RefSeq	NZ_CP001172
Uniprot ID	A0A009Q8T9_ACIBA, A0A009IIV5_ACIB9, A0A0J0ZK46_ACIBA, A0A086HY23_ACIBA, B0VA96_ACIBY, D0CDX8_ACIB2
KEGG ID	aby:ABAYE2667, abk:LX00_05320, abau:IX87_05690
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Abhydrolase_3	PF07859.15	1.4e-14	71..151

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-287	MNLIEQLQSKIEQARTLYKEFRLYDLGSFALNRFTPKDGFEQVLNVRYGLKPRHRLDIYRSTKRLAHRPLIVFVHGGAWQHGNKRDYLFIGEAFTKEGYDVAVINYQLAPKNIFPSYVDDLTQALNYLHQNQEKLEISTENIVLMGHSAGAFNVMSAVYHPKPNTIQCLGNIKAIFGLAGPYHFDYKGDPLAEDAFDQSISYKEVMPYYFVNQNHIKHYLLMAENDQLVKKENTFDLDQALRQSGNHSHIAVIPKTGHITILASLSSFVSHYFRTKRTILHFLDEVF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9955	-	-

Protein sequence: 288 a.a. .

                                    >IMU00305 NZ_CP001172:c2670331-2669468 [Acinetobacter baumannii AB307-0294]
MNLIEQLQSKIEQARTLYKEFRLYDLGSFALNRFTPKDGFEQVLNVRYGLKPRHRLDIYRSTKRLAHRPLIVFVHGGAWQ
HGNKRDYLFIGEAFTKEGYDVAVINYQLAPKNIFPSYVDDLTQALNYLHQNQEKLEISTENIVLMGHSAGAFNVMSAVYH
PKPNTIQCLGNIKAIFGLAGPYHFDYKGDPLAEDAFDQSISYKEVMPYYFVNQNHIKHYLLMAENDQLVKKENTFDLDQA
LRQSGNHSHIAVIPKTGHITILASLSSFVSHYFRTKRTILHFLDEVF*

                                

Nucleotide sequence: 864 bp .

                                    >IMU00305 NZ_CP001172:c2670331-2669468 [Acinetobacter baumannii AB307-0294]
ATGAACCTGATTGAACAATTACAAAGCAAAATTGAGCAGGCGAGAACTCTTTATAAAGAGTTCCGTCTGTATGATTTGGG
GAGCTTTGCTTTAAACCGTTTTACACCAAAAGATGGTTTTGAGCAGGTTTTAAATGTGCGTTATGGCTTAAAGCCTCGCC
ATCGTCTAGATATTTATCGTTCTACTAAGCGCTTAGCTCATCGACCACTCATTGTTTTTGTACATGGTGGTGCTTGGCAA
CATGGTAATAAACGAGACTATTTATTTATAGGTGAAGCGTTTACGAAAGAAGGTTATGACGTTGCTGTCATTAACTATCA
GCTCGCACCGAAGAATATTTTTCCAAGTTATGTAGATGACTTAACGCAAGCGTTAAACTATTTACACCAGAACCAAGAAA
AACTGGAAATCTCGACAGAGAATATTGTGCTTATGGGGCATTCGGCAGGTGCATTTAATGTGATGTCTGCTGTTTATCAC
CCAAAACCAAATACAATTCAGTGTTTAGGTAATATTAAAGCAATTTTTGGTTTGGCAGGTCCTTATCATTTTGATTATAA
AGGTGATCCGCTTGCCGAAGATGCTTTTGATCAAAGCATTTCTTATAAAGAAGTGATGCCATATTATTTTGTAAATCAAA
ATCATATTAAGCATTATCTGCTCATGGCTGAAAATGATCAGCTTGTAAAAAAAGAAAATACCTTTGATCTTGATCAGGCA
CTTAGACAAAGCGGAAATCACAGTCATATCGCTGTGATTCCTAAAACAGGTCATATTACAATTTTGGCGAGTTTATCGAG
TTTTGTAAGTCATTACTTCCGAACTAAACGGACCATTTTGCACTTTCTGGATGAAGTATTCTAA

                                