SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01484
Effector type	Tde
Cognate immunity protein	IMU00305
Related T6SS (Type)	T6SS00312 (Type i4b)
Strain	Acinetobacter baumannii AB307-0294
Replicon	chromosome
Sequence	Protein sequence (236 a.a.); Nucleotide sequence (708 bp)
Description	Rhs1 shares amino acid identity with several previously characterized T6SS Rhs family nuclease effectors.
Reference	[1] Fitzsimons TC, Lewis JM, Wright A, et al. Identification of Novel Acinetobacter baumannii Type VI Secretion System Antibacterial Effector and Immunity Pairs. Infect Immun. 2018 Jul 23;86(8). pii: e00297-18. doi: 10.1128/IAI.00297-18. Print 2018 Aug. PMID: 29735524

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ABBFA_RS12570
Coordinates (Strand)	2670335..2671042 (-)
NCBI ID	446842107
RefSeq	NZ_CP001172
Uniprot ID	A0A009PDZ0_ACIBA, A0A009HNH4_ACIB9, A0A009GXB3_ACIBA, A0A009JLM7_ACIBA, A0A0E1PSS2_ACIBA, A0A0J1CU63_ACIBA, A0A2S4T6C1_9GAMM, B0VA97_ACIBY, D0CDX7_ACIB2, L9MAV6_9GAMM
KEGG ID	aby:ABAYE2668
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
BON	PF04972.19	6.8e-11	60..113

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-235	VLKRIAVTALCVASLSGCASFISGGTGTAPVGTDSGVRSLGQVFIDSSIKRTANINLYKLDQRFKQSRINIESFHSTVLLTGQVPDPYLKQLAEDNVKAMSDVKAVHNYITVGNKVSYNTIMQDAGVTANTRALLMKAPVVSDSKVLVHTEDGVLYVMGRLNTAEINDLNNVLQNVGNVTKIVTLIDNIDLAPAPAASTASATTTPVINNVLAQPTVQTPVAIDPDQTDPASSAQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9953	pos: 17-18. LSG-CA.	VLKRIAVTALCVASLSG

Protein sequence: 236 a.a. .

                                    >EFF01484 NZ_CP001172:c2671042-2670335 Type VI DNase and RNase Effector (Tde) [Acinetobacter baumannii AB307-0294]
VLKRIAVTALCVASLSGCASFISGGTGTAPVGTDSGVRSLGQVFIDSSIKRTANINLYKLDQRFKQSRINIESFHSTVLL
TGQVPDPYLKQLAEDNVKAMSDVKAVHNYITVGNKVSYNTIMQDAGVTANTRALLMKAPVVSDSKVLVHTEDGVLYVMGR
LNTAEINDLNNVLQNVGNVTKIVTLIDNIDLAPAPAASTASATTTPVINNVLAQPTVQTPVAIDPDQTDPASSAQ*

                                

Nucleotide sequence: 708 bp .

                                    >EFF01484 NZ_CP001172:c2671042-2670335 Type VI DNase and RNase Effector (Tde) [Acinetobacter baumannii AB307-0294]
GTGTTGAAACGTATTGCTGTAACAGCGCTTTGTGTCGCAAGTTTGTCAGGTTGCGCAAGTTTTATTTCTGGTGGAACAGG
TACAGCTCCAGTTGGAACAGATAGTGGTGTACGTAGCTTAGGCCAAGTATTTATTGATTCTTCAATTAAGCGTACAGCGA
ATATTAACCTCTATAAGCTTGATCAGCGTTTTAAACAATCTCGTATTAACATCGAAAGCTTCCATAGCACAGTATTGTTA
ACTGGACAGGTGCCAGACCCATACTTAAAACAGTTGGCAGAAGACAACGTTAAAGCCATGAGTGATGTGAAAGCTGTACA
TAACTACATTACCGTGGGTAACAAAGTAAGCTATAACACGATTATGCAAGATGCTGGGGTAACCGCAAATACACGTGCTT
TATTAATGAAAGCGCCAGTAGTATCGGACAGTAAAGTACTGGTTCATACCGAAGATGGTGTTTTATATGTGATGGGCCGT
TTAAACACAGCTGAAATTAATGATTTAAACAATGTGCTTCAAAATGTCGGTAACGTGACTAAGATTGTAACGCTCATTGA
TAATATTGATTTAGCGCCTGCTCCGGCAGCGAGTACTGCCAGCGCAACAACTACACCAGTAATTAATAACGTTCTTGCTC
AACCAACAGTTCAAACTCCTGTTGCAATTGATCCGGACCAAACAGATCCAGCATCTTCTGCACAATAA

                                