SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00297
Cognate effector	EFF01471
Related T6SS (Type)	T6SS00061 (Type i4b)
Strain	Acinetobacter baylyi ADP1
Replicon	chromosome
Sequence	Protein sequence (328 a.a.); Nucleotide sequence (984 bp)
Description	Tsi2b
Reference	[1] Ringel PD, Hu D, Basler M. The Role of Type VI Secretion System Effectors in Target Cell Lysis and Subsequent Horizontal Gene Transfer. Cell Rep. 2017 Dec 26;21(13):3927-3940. doi: 10.1016/j.celrep.2017.12.020. PMID: 29281838

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ACIAD3113 (Tsi2b)
Coordinates (Strand)	3039389..3040372 (+)
NCBI ID	50086117
RefSeq	NC_005966
Uniprot ID	UPI00003B7CD3
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
FGE-sulfatase	PF03781.18	3.2e-31	59..271

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-327	MTYKKMMKQNLVIFLISSLSLSACAKSDATQKTSTPVTKQDLSSEQKKQLQQLLEKTKKNLIFVKGGSFKMGDFGPEHSIDKLPYDSNGDSKPLHKVTLDDFYLSATKATYADFDVYTDVTGQKPIGNFNEVTKKYRIPTSAAGINWQQARNYCQWLGTQLNLKMDLPTEAQWEYAARNRGQYILYPTDNGKIDNGRNVWSFEQRQASKKPFKVTKSISLLQKFPPTPLGLYDMITDNYEWILDWYDPEYYSKSPEKNPQGPNTGTLKVVRSSAPADGQPLQTFTGKTISRHAMDPMADPELVKESKNQKYYFDFNLNNSVRCVANP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9956	pos: 23-24. LSA-CA.	MTYKKMMKQNLVIFLISSLSLSA

Protein sequence: 328 a.a. .

                                    >IMU00297 NC_005966:3039389-3040372 [Acinetobacter baylyi ADP1]
MTYKKMMKQNLVIFLISSLSLSACAKSDATQKTSTPVTKQDLSSEQKKQLQQLLEKTKKNLIFVKGGSFKMGDFGPEHSI
DKLPYDSNGDSKPLHKVTLDDFYLSATKATYADFDVYTDVTGQKPIGNFNEVTKKYRIPTSAAGINWQQARNYCQWLGTQ
LNLKMDLPTEAQWEYAARNRGQYILYPTDNGKIDNGRNVWSFEQRQASKKPFKVTKSISLLQKFPPTPLGLYDMITDNYE
WILDWYDPEYYSKSPEKNPQGPNTGTLKVVRSSAPADGQPLQTFTGKTISRHAMDPMADPELVKESKNQKYYFDFNLNNS
VRCVANP*

                                

Nucleotide sequence: 984 bp .

                                    >IMU00297 NC_005966:3039389-3040372 [Acinetobacter baylyi ADP1]
ATGACTTATAAAAAGATGATGAAACAAAATCTTGTAATTTTTTTAATTTCAAGCCTTTCGCTTAGTGCATGTGCCAAATC
AGATGCTACTCAAAAAACTTCTACACCAGTAACAAAACAAGACCTTTCTTCTGAACAAAAAAAGCAATTACAACAATTGC
TTGAGAAAACAAAAAAAAATCTGATCTTTGTGAAAGGTGGAAGTTTTAAGATGGGTGACTTTGGTCCAGAACATAGTATT
GATAAACTCCCATACGACAGTAATGGAGATAGCAAACCTCTTCATAAGGTCACTTTGGATGATTTTTATTTAAGTGCCAC
CAAAGCCACTTATGCCGATTTTGATGTATATACTGATGTCACAGGACAAAAACCTATTGGCAATTTTAACGAAGTAACAA
AAAAATATCGTATTCCAACTTCTGCAGCAGGGATCAACTGGCAACAAGCTCGAAATTATTGCCAATGGCTTGGTACGCAA
CTCAATTTAAAGATGGATTTACCAACAGAAGCACAATGGGAATATGCGGCACGTAACCGCGGACAATATATTCTATATCC
AACAGATAATGGAAAAATTGATAATGGCCGTAATGTTTGGAGCTTTGAACAAAGACAAGCATCTAAAAAACCATTTAAAG
TAACTAAAAGTATTTCCTTATTACAAAAATTTCCACCAACACCATTGGGTCTATACGATATGATCACAGATAATTATGAG
TGGATACTGGACTGGTATGATCCTGAGTATTACAGTAAATCTCCAGAGAAAAACCCTCAAGGTCCAAACACGGGAACTTT
AAAAGTAGTACGAAGTTCTGCACCAGCAGATGGACAACCATTGCAAACATTTACTGGCAAAACTATTAGTAGACATGCAA
TGGATCCGATGGCAGATCCTGAATTAGTCAAAGAGTCTAAGAATCAGAAATATTACTTTGATTTTAACTTAAATAATTCA
GTACGTTGTGTAGCCAATCCTTAA

                                