SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00296
Cognate effector	EFF01471
Related T6SS (Type)	T6SS00061 (Type i4b)
Strain	Acinetobacter baylyi ADP1
Replicon	chromosome
Sequence	Protein sequence (328 a.a.); Nucleotide sequence (984 bp)
Description	Tsi2a
Reference	[1] Ringel PD, Hu D, Basler M. The Role of Type VI Secretion System Effectors in Target Cell Lysis and Subsequent Horizontal Gene Transfer. Cell Rep. 2017 Dec 26;21(13):3927-3940. doi: 10.1016/j.celrep.2017.12.020. PMID: 29281838

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ACIAD3112 (Tsi2a)
Coordinates (Strand)	3038267..3039250 (+)
NCBI ID	50086116
RefSeq	NC_005966
Uniprot ID	UPI00003B7CD2
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Loading, please wait

Domain	Pfam ID	E-value	Aligned region
FGE-sulfatase	PF03781.18	2.3e-30	54..261

Showing 1 to 1 of 1 rows

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Loading, please wait

Prediction	Region	Sequence
Outside	1-327	MTYKKMMKQNLVIFLFSTLALNACAKSRETSTKAAQPSAELQQKIQKLVEKTQKNMIFVEGGSFMMGDFGHVTRKDKLPITGLYVAMPLHKVTLDSFSLNAYKTTFDEFDIYTEATGQKKIAMQEISKKLRVPNAPAGVSWQQAHDYCQWLGQQVGIPMTLPTEAQWEYAARNRGQYVIYPTDNGEYEPGRNVWSDEQRREFTKSMPDIHGVKVPTLGRFPPTPLGFYDLATDSYEWMSDWFDPQYYKHSPEKNPQGPATGTLKSVRSTYQEAGVLDLKIGGSVTTIYRYGVEPNPKFDKDDNAFNTINPNSATAVRCASNSHKPFI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Loading, please wait

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9950	pos: 23-24. LNA-CA.	MTYKKMMKQNLVIFLFSTLALNA

Protein sequence: 328 a.a. .

                                    >IMU00296 NC_005966:3038267-3039250 [Acinetobacter baylyi ADP1]
MTYKKMMKQNLVIFLFSTLALNACAKSRETSTKAAQPSAELQQKIQKLVEKTQKNMIFVEGGSFMMGDFGHVTRKDKLPI
TGLYVAMPLHKVTLDSFSLNAYKTTFDEFDIYTEATGQKKIAMQEISKKLRVPNAPAGVSWQQAHDYCQWLGQQVGIPMT
LPTEAQWEYAARNRGQYVIYPTDNGEYEPGRNVWSDEQRREFTKSMPDIHGVKVPTLGRFPPTPLGFYDLATDSYEWMSD
WFDPQYYKHSPEKNPQGPATGTLKSVRSTYQEAGVLDLKIGGSVTTIYRYGVEPNPKFDKDDNAFNTINPNSATAVRCAS
NSHKPFI*

                                

Nucleotide sequence: 984 bp .

                                    >IMU00296 NC_005966:3038267-3039250 [Acinetobacter baylyi ADP1]
ATGACTTATAAAAAGATGATGAAACAAAATCTTGTAATTTTTTTATTTTCAACTCTTGCTTTAAATGCCTGTGCAAAATC
GAGAGAAACATCCACCAAAGCAGCTCAGCCTTCAGCCGAATTACAACAAAAAATTCAAAAGCTCGTTGAGAAAACCCAAA
AAAATATGATTTTTGTTGAGGGTGGAAGCTTTATGATGGGTGATTTTGGTCATGTCACACGTAAAGATAAATTACCTATC
ACTGGCCTTTATGTAGCCATGCCTTTACATAAAGTCACCCTCGATAGTTTTAGTTTAAATGCGTATAAAACCACCTTTGA
TGAGTTTGATATTTATACTGAAGCGACTGGGCAGAAAAAAATTGCAATGCAAGAAATTTCCAAAAAGTTACGCGTACCAA
ATGCACCCGCAGGTGTGAGCTGGCAACAAGCACACGATTACTGTCAATGGCTAGGCCAACAAGTTGGAATACCTATGACC
CTACCAACCGAAGCGCAATGGGAATATGCTGCCAGAAACCGTGGGCAATATGTTATCTATCCAACGGATAATGGTGAATA
TGAGCCGGGACGAAATGTTTGGAGTGATGAACAAAGACGTGAATTTACTAAATCTATGCCCGATATTCATGGAGTAAAAG
TTCCGACACTTGGTCGGTTTCCTCCGACACCTTTAGGCTTTTATGACTTAGCAACTGATAGCTATGAATGGATGTCGGAT
TGGTTTGATCCTCAATATTATAAGCATTCACCAGAGAAAAACCCTCAAGGCCCAGCGACAGGGACACTCAAATCGGTTCG
AAGCACTTATCAGGAGGCTGGTGTTTTAGATCTAAAAATTGGCGGTAGTGTAACAACAATTTATAGATATGGTGTGGAAC
CTAATCCTAAATTTGATAAGGATGACAATGCTTTCAATACTATTAACCCAAATAGTGCAACCGCTGTAAGATGCGCCTCT
AACTCGCATAAACCATTTATCTAA

                                