Detailed information of immunity protein


Summary

Immunity protein ID IMU00295 experimental
Cognate effector EFF01472 experimental
Related T6SS (Type) T6SS00061 experimental (Type i4b)
Strain Acinetobacter baylyi ADP1
Replicon chromosome
Sequence Protein sequence (353 a.a.); Nucleotide sequence (1059 bp)
Description Tli1 carries a predicted cleavable N-terminal signal sequence as is common for the cognate phospholipase immunity proteins (Russell et al., 2013).
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ACIAD3426 (Tli1)
Coordinates (Strand) 3341328..3342386 (-)
NCBI ID 50086394
RefSeq NC_005966
Uniprot ID UPI00003B7DDC
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Tli4_C PF18426.3 2e-16 183..347
Tli4_N PF18443.3 8.8e-05 39..152
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-352 MKLLKRLTLCLLTTVSFSACSNVEKEQEGIKVIDYRNPKKVCFGRIELTVPKETEVEYSNFNYNGSNIEVDESVKTYEAYQKLINDKIEYLKHEPHETEGVLLKQEMPGPVNQLNRSVSHIIVFRDGLYVTSAYEVYGYLYLGPKRLVVLKSGASNDLLNEAIEDMKQTLRSIKIKSAKGEDQAGLCWKDFFIVDDMSKNIPFGGGYLHFKFPSYPQVRADVVYRVTYDSDLHLIEMMKKNQKELPAIAKALMSIENLREHKKIINGLGGEEVLNHMSRRGYFERGYEVGEWQYLGTLDNHNDPYIQFSFDSADMVRPDDPLNSTISQKEALRLNDFILNSIRITPNNEKEK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9990 pos: 19-20. FSA-CS. MKLLKRLTLCLLTTVSFSA


  Protein sequence: 353 a.a.    .

>IMU00295 NC_005966:c3342386-3341328 [Acinetobacter baylyi ADP1]
MKLLKRLTLCLLTTVSFSACSNVEKEQEGIKVIDYRNPKKVCFGRIELTVPKETEVEYSNFNYNGSNIEVDESVKTYEAY
QKLINDKIEYLKHEPHETEGVLLKQEMPGPVNQLNRSVSHIIVFRDGLYVTSAYEVYGYLYLGPKRLVVLKSGASNDLLN
EAIEDMKQTLRSIKIKSAKGEDQAGLCWKDFFIVDDMSKNIPFGGGYLHFKFPSYPQVRADVVYRVTYDSDLHLIEMMKK
NQKELPAIAKALMSIENLREHKKIINGLGGEEVLNHMSRRGYFERGYEVGEWQYLGTLDNHNDPYIQFSFDSADMVRPDD
PLNSTISQKEALRLNDFILNSIRITPNNEKEK*

  Nucleotide sequence: 1059 bp    .

>IMU00295 NC_005966:c3342386-3341328 [Acinetobacter baylyi ADP1]
ATGAAATTATTAAAGCGCTTAACCCTTTGCTTATTAACAACAGTTTCTTTTAGTGCATGTTCAAATGTTGAAAAAGAACA
AGAAGGAATTAAAGTGATCGATTATCGTAATCCTAAAAAAGTGTGTTTCGGCCGAATTGAATTAACGGTTCCGAAAGAAA
CAGAAGTTGAATACAGTAACTTCAACTACAACGGCAGTAATATAGAAGTAGATGAAAGTGTAAAAACATACGAAGCATAT
CAAAAATTGATTAATGACAAGATTGAGTATTTAAAGCATGAGCCGCATGAAACAGAAGGCGTTTTATTAAAACAAGAAAT
GCCAGGCCCAGTAAATCAGCTTAACCGTTCTGTTAGTCATATTATTGTTTTTCGAGATGGGTTGTATGTGACATCAGCTT
ATGAAGTTTATGGTTACCTTTATCTAGGCCCGAAAAGATTAGTTGTTCTTAAAAGTGGAGCAAGTAATGATTTATTGAAT
GAGGCTATTGAGGACATGAAACAAACTTTAAGAAGTATAAAAATAAAATCGGCTAAAGGCGAAGATCAAGCCGGTTTATG
TTGGAAGGATTTTTTTATTGTAGACGATATGAGCAAAAACATTCCGTTTGGGGGAGGATATTTACATTTCAAATTCCCTA
GTTATCCGCAAGTTCGGGCTGATGTCGTATATCGTGTAACCTATGACTCGGATTTGCACTTAATTGAAATGATGAAAAAG
AACCAGAAAGAGTTACCTGCCATTGCGAAAGCCTTGATGAGCATTGAGAATCTCAGAGAACATAAAAAGATAATCAATGG
TTTGGGTGGTGAAGAGGTGCTAAATCATATGTCCAGACGTGGATATTTTGAAAGAGGCTATGAAGTAGGAGAATGGCAGT
ATCTAGGCACACTAGATAACCATAATGACCCTTATATTCAGTTTAGTTTTGATAGTGCAGACATGGTAAGACCTGACGAC
CCACTCAATTCAACAATAAGCCAGAAAGAAGCTCTTCGACTGAATGATTTTATTTTAAACAGTATCCGAATTACACCGAA
TAATGAAAAAGAGAAATAA