Detailed information of effector


Summary

Effector ID EFF18620 experimental
Effector type Tre
Cognate immunity protein IMU17296 experimental
Related T6SS (Type) T6SS00050 experimental (Type i1)
Strain Photorhabdus laumondii subsp. laumondii TTO1
Replicon chromosome
Sequence Protein sequence (1343 a.a.); Nucleotide sequence (4029 bp)
Description The Plu0353 protein presents the canonical architectural organization of Rhs proteins: a N-terminal domain (RhsNT) that comprises a PAAR motif, the central YD-repeat shell (RhsShell) and the C-terminal toxin domain (RhsCT).Tre23 arrests translation in a NAD+-dependent man- ner, and that Tri23 inhibits its activity via direct contact with Tre23. Tre23 transfers ADP-ribose on the 23S rRNA on actively translating ribosomes and modifies 23S rRNAGTPase-associated center helix 44 hence impairing the GTPase activity of elongation factor G.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) Plu0353 (Tre23)
Coordinates (Strand) 373064..377092 (-)
NCBI ID CAE12648.1
RefSeq NC_005126
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
PAAR_motif PF05488.15 1.1e-18 117..180
DUF6531 PF20148.1 1.9e-11 287..357
RHS_repeat PF05593.16 1.6e-07 833..870
RHS_repeat PF05593.16 5.5e-06 558..595
RHS_repeat PF05593.16 1.7e-05 874..909
RHS_repeat PF05593.16 2.8e-05 1139..1168
RHS_repeat PF05593.16 3e-05 644..680
RHS_repeat PF05593.16 3.4e-05 686..719
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-42 MSLGDEIVTRIARVGARHAVHVSPPQGSPGPAAAREGDPIKH
Transmembrane helix 43-65 ASFLGALAGAITGALIGAAVFAA
Inside 66-71 ASALVG
Transmembrane helix 72-94 LTILTGGLATVAVIALGTAATFA
Outside 95-1343 LGDVISAASSAVTNMVDSIGPPSGTLTSGSPNVFIEGQPAARATTDIAVCSKHPAPPLIAQGSETVLINGSPAARVDDKLACGATIKSGAKTVFIGSGQGTYLAVADEFSAWERAILIAVEFLVPPSRGALKGLGKLFTKPGQGIQGVLKGAKAGAKKAKALLSNRISCAKKAFRETEGAKRYREATKKFFTGDPIDVTTGQLFDQRTDITLGQTLPLVFLRSWVPEEQGLLGPGWTDSFSECALATGDRVEIRTTEGASLYFALPAAYTHSVNPDHPDFTLSRGEQGYILRHRDSPVSKYFTLPHPSSASKEAPRRWLLTEHRDVYDNRLRFIYNKHCQLTQVLHSDGPELTLLYNLRGQLTEIRRTDERLQEVMARYHYHDNGRLAEADSTQNFHLYYEYNAQGLISRWSDGDQTWVDYRYDKQGRCTDSVGAGGFYPVHLDYAPGITRSTTPQGHTTTGHYNDQQLITEIHTPCGGVTRYEYDRWGNLVRQILPEGETLTLTYLADTGRVTSLTEATGAVWQYSYEADSLQLTGMTDPLQRTWLPQYDEQGQPAGFIAPDGRKTTLTRNAFGLVTSETDPDGNSRTQEYDKHQRLVRVLDEENRTVSLGYDSQDRLRSLTAAGALWRWRYDRHHRVAVSDRPDNQLEHFTHDRHGNLTCWTDARGVKWQVEYGPFDLPVARRDGEGHRWQYRYDADTLQLTQVINPQGETYSYTLDADGRVITEQDYAGTQWHYRYDRSGNCIEKRDGEENVTRYDYDAARRLTTLHTPEGPTRYHYDSVGRLLTVDSPDSTLHFEYDGQDRIVREIQPHGEIQRHYPDNRTAERQLLTGETAPVTGPARFSGQTHPGRWQSRREVNRVGELITLTLAGQAPLTIERDDAGRDTGRYVDGGFILRQQYSLMGQLTAQRAGRNPYFFRPAEPDEIEGPAYAGVARRYEYDTALNLTAASDDGQQVNYLLNGNGQVISVGEGRTLREHYQYDETGYPSRRFDGVQEIMGETLYQEGHRLNWVGSHRFVYDRAGRMQEKQFLAEGCRLALTKYRWNSQNQLTGLITPDGIPWEYRYDAFGRRTEKRCIQSGKLTTYLWDGNVPAEIREYQHGRLKMIRHLVFDGWELVAQQTQAFTLNLDNRVELMAGEVQTQYAVSAPTGEPLALFDPAGKRVWRRPKQSLYGLRLGGYGENPQLDPGLRFAGQLFDEESGLFYNRFRYYLPEATCYLSPDPTGLWGGENTYRYVQNPTKFIDPLGLA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9051 - -


  Protein sequence: 1343 a.a.    .

>EFF18620 NC_005126:c377092-373064 Type VI ADP-Ribosyltransferase Effector (Tre) [Photorhabdus laumondii subsp. laumondii TTO1]
MSLGDEIVTRIARVGARHAVHVSPPQGSPGPAAAREGDPIKHASFLGALAGAITGALIGAAVFAAASALVGLTILTGGLA
TVAVIALGTAATFALGDVISAASSAVTNMVDSIGPPSGTLTSGSPNVFIEGQPAARATTDIAVCSKHPAPPLIAQGSETV
LINGSPAARVDDKLACGATIKSGAKTVFIGSGQGTYLAVADEFSAWERAILIAVEFLVPPSRGALKGLGKLFTKPGQGIQ
GVLKGAKAGAKKAKALLSNRISCAKKAFRETEGAKRYREATKKFFTGDPIDVTTGQLFDQRTDITLGQTLPLVFLRSWVP
EEQGLLGPGWTDSFSECALATGDRVEIRTTEGASLYFALPAAYTHSVNPDHPDFTLSRGEQGYILRHRDSPVSKYFTLPH
PSSASKEAPRRWLLTEHRDVYDNRLRFIYNKHCQLTQVLHSDGPELTLLYNLRGQLTEIRRTDERLQEVMARYHYHDNGR
LAEADSTQNFHLYYEYNAQGLISRWSDGDQTWVDYRYDKQGRCTDSVGAGGFYPVHLDYAPGITRSTTPQGHTTTGHYND
QQLITEIHTPCGGVTRYEYDRWGNLVRQILPEGETLTLTYLADTGRVTSLTEATGAVWQYSYEADSLQLTGMTDPLQRTW
LPQYDEQGQPAGFIAPDGRKTTLTRNAFGLVTSETDPDGNSRTQEYDKHQRLVRVLDEENRTVSLGYDSQDRLRSLTAAG
ALWRWRYDRHHRVAVSDRPDNQLEHFTHDRHGNLTCWTDARGVKWQVEYGPFDLPVARRDGEGHRWQYRYDADTLQLTQV
INPQGETYSYTLDADGRVITEQDYAGTQWHYRYDRSGNCIEKRDGEENVTRYDYDAARRLTTLHTPEGPTRYHYDSVGRL
LTVDSPDSTLHFEYDGQDRIVREIQPHGEIQRHYPDNRTAERQLLTGETAPVTGPARFSGQTHPGRWQSRREVNRVGELI
TLTLAGQAPLTIERDDAGRDTGRYVDGGFILRQQYSLMGQLTAQRAGRNPYFFRPAEPDEIEGPAYAGVARRYEYDTALN
LTAASDDGQQVNYLLNGNGQVISVGEGRTLREHYQYDETGYPSRRFDGVQEIMGETLYQEGHRLNWVGSHRFVYDRAGRM
QEKQFLAEGCRLALTKYRWNSQNQLTGLITPDGIPWEYRYDAFGRRTEKRCIQSGKLTTYLWDGNVPAEIREYQHGRLKM
IRHLVFDGWELVAQQTQAFTLNLDNRVELMAGEVQTQYAVSAPTGEPLALFDPAGKRVWRRPKQSLYGLRLGGYGENPQL
DPGLRFAGQLFDEESGLFYNRFRYYLPEATCYLSPDPTGLWGGENTYRYVQNPTKFIDPLGLA

  Nucleotide sequence: 4029 bp    .

>EFF18620 NC_005126:c377092-373064 Type VI ADP-Ribosyltransferase Effector (Tre) [Photorhabdus laumondii subsp. laumondii TTO1]
ATGTCACTTGGTGATGAAATCGTCACCCGGATAGCGCGGGTTGGCGCCCGGCACGCGGTGCACGTCTCCCCGCCACAGGG
CTCCCCCGGTCCGGCCGCCGCCCGTGAGGGCGACCCGATTAAACATGCCAGCTTCCTGGGGGCGCTGGCGGGTGCCATTA
CCGGCGCACTGATAGGGGCGGCGGTCTTTGCCGCGGCATCGGCACTGGTGGGACTGACCATTCTCACCGGGGGATTAGCC
ACCGTCGCGGTCATTGCGCTGGGAACCGCCGCCACATTTGCCCTGGGCGATGTCATCAGCGCCGCCAGTTCGGCCGTCAC
GAACATGGTGGACAGCATCGGCCCGCCGAGCGGCACCCTGACGAGTGGCTCCCCCAATGTCTTTATCGAAGGCCAACCGG
CGGCCAGAGCCACCACCGATATCGCCGTTTGCAGTAAACACCCGGCGCCGCCCCTGATTGCCCAGGGCAGTGAAACCGTG
TTGATTAACGGCTCGCCGGCGGCCCGGGTGGATGACAAACTGGCGTGTGGCGCCACCATAAAATCCGGGGCCAAAACGGT
GTTTATCGGCTCCGGTCAGGGAACTTACCTGGCAGTCGCCGACGAGTTTTCCGCCTGGGAACGGGCGATACTGATAGCGG
TTGAATTTCTGGTGCCGCCGTCACGAGGCGCCCTTAAGGGGCTGGGTAAGCTGTTTACCAAACCCGGTCAGGGAATTCAG
GGGGTATTGAAAGGCGCGAAGGCGGGTGCTAAAAAGGCTAAGGCGCTCCTCTCAAATCGAATTTCCTGTGCCAAAAAAGC
TTTCAGAGAAACTGAAGGGGCAAAACGTTACCGGGAAGCGACGAAAAAATTCTTTACCGGCGACCCGATTGACGTCACCA
CCGGCCAGTTGTTTGACCAGCGGACCGACATCACCCTCGGCCAGACCCTGCCGCTGGTGTTTCTGCGCAGCTGGGTGCCG
GAGGAACAGGGCTTACTGGGGCCGGGCTGGACGGACAGCTTCTCCGAATGCGCCCTTGCCACCGGCGACCGGGTAGAAAT
CCGCACCACCGAAGGGGCTTCTCTCTATTTTGCCTTACCGGCCGCTTATACCCACAGTGTTAACCCGGACCACCCGGACT
TTACCCTGAGCCGGGGAGAACAGGGATATATTCTCCGTCACCGTGATTCACCGGTCAGCAAATATTTTACCCTGCCCCAT
CCGTCATCCGCCTCAAAAGAGGCACCCCGGCGCTGGTTGCTGACGGAACACCGGGACGTGTATGACAACCGGCTGCGGTT
TATCTATAACAAGCACTGTCAACTGACACAGGTTCTCCACAGTGACGGGCCGGAGTTGACGCTGCTTTATAACCTGCGCG
GACAACTGACAGAGATACGCCGCACCGATGAAAGGTTACAGGAAGTGATGGCCCGTTATCACTACCATGACAACGGCCGG
CTGGCGGAAGCGGACAGTACTCAGAACTTCCACCTGTACTATGAATATAACGCGCAGGGCCTGATAAGCCGCTGGTCCGA
TGGCGACCAGACCTGGGTGGATTACCGTTATGATAAACAGGGGCGCTGTACTGACAGTGTGGGAGCCGGCGGCTTCTATC
CGGTCCATCTGGATTATGCCCCCGGCATAACCCGCTCCACCACCCCACAGGGGCACACCACCACCGGGCATTACAACGAC
CAGCAGTTAATCACTGAAATCCACACCCCGTGCGGCGGCGTGACCCGTTATGAATACGACCGCTGGGGCAATCTGGTCAG
ACAGATATTACCGGAAGGGGAAACCCTGACGCTGACTTATCTGGCCGATACCGGCCGGGTCACTTCGCTGACGGAGGCGA
CCGGCGCGGTATGGCAATACAGCTATGAGGCTGACAGCCTGCAACTGACCGGCATGACCGACCCGCTGCAACGCACCTGG
CTGCCCCAGTATGATGAACAGGGCCAGCCGGCCGGATTTATTGCGCCGGACGGCCGGAAAACCACGCTGACCCGCAATGC
TTTCGGGCTGGTGACATCCGAAACAGACCCGGACGGCAACAGCCGCACACAGGAATATGATAAGCATCAGCGGCTGGTGC
GGGTGCTGGATGAAGAGAACCGGACCGTCAGTCTGGGTTATGACAGCCAGGACCGGCTGCGCAGCCTGACCGCGGCAGGG
GCGCTGTGGCGCTGGCGTTACGACCGTCATCACCGGGTGGCGGTCAGCGACCGGCCGGACAACCAGCTGGAACACTTCAC
CCATGACCGCCACGGGAACTTAACCTGCTGGACCGATGCCCGCGGTGTGAAATGGCAGGTCGAGTATGGCCCGTTTGACC
TGCCGGTCGCCCGGCGTGACGGCGAGGGGCATCGCTGGCAGTATCGTTATGATGCCGACACGCTGCAACTGACGCAGGTG
ATTAACCCGCAGGGGGAAACCTACAGCTACACCCTGGATGCCGACGGGCGGGTTATCACCGAGCAGGATTATGCCGGCAC
GCAGTGGCATTATCGCTATGACCGGTCGGGGAACTGCATTGAAAAGCGGGACGGGGAAGAGAACGTGACCCGCTATGACT
ATGATGCTGCCCGCCGGCTGACGACGCTGCACACCCCGGAAGGCCCCACCCGTTATCATTACGACAGCGTGGGCCGGCTG
TTAACCGTGGACTCCCCGGACAGCACCCTGCATTTTGAATATGACGGGCAGGACCGGATAGTGCGGGAAATTCAGCCGCA
CGGAGAAATTCAGCGTCACTACCCGGATAACCGCACCGCAGAACGGCAGTTGCTGACCGGCGAAACAGCGCCGGTCACCG
GCCCGGCCCGGTTTAGCGGTCAGACCCACCCCGGCCGCTGGCAGAGCCGGCGGGAAGTTAACCGGGTGGGAGAACTGATT
ACGCTGACGCTGGCAGGGCAGGCACCCCTGACCATCGAACGGGATGACGCCGGCCGGGATACGGGACGTTATGTGGACGG
GGGATTTATTCTGCGCCAGCAGTACAGCCTGATGGGCCAGTTAACCGCGCAACGGGCCGGCCGCAATCCCTATTTTTTCC
GGCCGGCTGAACCGGACGAGATTGAGGGACCCGCTTACGCGGGCGTGGCCCGCCGGTATGAATACGATACCGCGCTGAAT
CTGACCGCCGCCAGTGATGACGGGCAGCAGGTCAATTATCTCCTCAACGGCAACGGCCAGGTGATTTCGGTGGGGGAGGG
CCGGACACTGCGCGAGCATTATCAGTATGATGAAACGGGGTACCCGTCAAGACGGTTTGACGGGGTGCAGGAGATTATGG
GGGAAACCCTCTATCAGGAGGGCCATCGCCTGAACTGGGTGGGCTCGCACCGGTTTGTTTACGACCGGGCCGGCCGCATG
CAGGAGAAACAGTTTTTAGCGGAAGGGTGCCGGCTGGCGCTGACGAAATACCGGTGGAACAGCCAGAATCAGCTGACCGG
GCTTATCACCCCGGACGGGATACCGTGGGAATATCGCTATGACGCCTTCGGACGGCGGACAGAAAAACGGTGTATCCAGA
GCGGTAAGCTGACCACCTATCTGTGGGATGGGAATGTGCCGGCGGAAATCCGCGAATATCAGCACGGCCGGCTGAAAATG
ATCCGCCATCTGGTGTTTGACGGCTGGGAACTGGTGGCGCAGCAGACGCAGGCGTTTACGCTTAATCTGGATAACCGGGT
GGAGCTGATGGCCGGGGAGGTGCAGACCCAGTATGCGGTCAGCGCCCCGACCGGCGAGCCGCTGGCGCTGTTCGACCCGG
CAGGCAAACGGGTGTGGCGACGACCGAAGCAGTCCCTGTACGGGCTGCGGCTGGGCGGATACGGCGAAAACCCGCAGCTG
GACCCGGGCCTGAGATTTGCGGGGCAACTTTTTGATGAAGAAAGTGGTTTATTTTATAACCGATTTCGCTATTATTTGCC
GGAAGCGACGTGCTACCTCAGCCCTGATCCAACTGGCCTCTGGGGTGGTGAGAATACCTATCGCTATGTGCAAAATCCCA
CGAAATTTATTGACCCTCTAGGGCTTGCT