SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01806
Effector type	Tse
Cognate immunity protein	IMU38221
Related T6SS (Type)	T6SS01046 (Type i4b)
Strain	Acinetobacter baumannii strain ATCC 17978
Replicon	chromosome
Sequence	Protein sequence (1002 a.a.); Nucleotide sequence (3006 bp)
Description	-
Reference	[1] Weber BS, Hennon SW, Wright MS, et al. Genetic Dissection of the Type VI Secretion System in Acinetobacter and Identification of a Novel Peptidoglycan Hydrolase, TagX, Required for Its Biogenesis. mBio. 2016 Oct 11;7(5). pii: e01253-16. doi: 10.1128/mBio.01253-16. PMID: 27729508 [2] Lopez J, Le NH, Moon KH, et al. Formylglycine-Generating Enzyme-Like Proteins Constitute a Novel Family of Widespread Type VI Secretion System Immunity Proteins. J Bacteriol. 2021 Oct 12;203(21):e0028121. doi: 10.1128/JB.00281-21. Epub 2021 Aug 16. PMID: 34398661

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ACX60_11695 (tse3)
Coordinates (Strand)	2455521..2458526 (-)
NCBI ID	446993254
RefSeq	NZ_CP012004
Uniprot ID	UPI0001785DCB
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1001	MNPYTIFEYTIVFYNQRNKELPNVKYSIIFFCEDGTKKTYEGATNPKGQTKPIPLNQNGKLHIFVEGHETIFSPKKTIKPILAEGSNIVEVNDIRLKSNTSFLTKAQYELMQKKTSKDLEELRKNAKAVKQKNFFNLSKIRPTFPLDLAEELNERINMSYEEYRKKNTHIVKKTKYLKFKNYALYRFVDSAGNGIPIVDYQIFAQGQSRPLINARPGPPDKKGYTQLVYTHLKSRVTYTLGSARKNSEWYEPITCVDKQTIYQIIFPTSIAVTQPDINHKENMEARQKPPIVINPTTNEVLILPSAVYAEFDKKTKILSDAVREVHKSNANLIKAIQSRDLDEIKELEKRLNINQEKAIEKINGEFKQTADLREVWVVETTGKTNEKISKYNLKRRYLKVTEYEELKAKRRNPQTEVDVTANQYTVQQPAQIRSSFEKLSQQLLTVKGNAGSDEKAVYNLIGGLGGEIAEEYKNSRDVTVTQEAQWMRMVAGASGEGSISAAPKGVSIKTSGDMSAKWTLFEGVKEWRKFYPCETGWKLEYDNYDLGTIRFLIGAEISGFSGANLGIAGNLSVDISHQGAAQVIKAVVRPPERSMSQMVDRNKKPMFQPAQGSLKIIGNNNQENAQNQGNISINAFAGVQIQGLLKGAVEWFKPKGDGSGEGEFVAIASAAAGGGVSAGVGAQGQFQIGYDQTSGNFKILVAAHLCWGMGAKGVASFVVGTEHLLSYLGFIKSQVAHAGFKTLLYINEAAFLLAAQVLAYCIGENHPVISDINRIAASYGDWIRRLDIDQGRYKTAQNINSSSGKKELLYATPETKGILLYAVTHWTDRTAPIFDMNVKFSDMKIEFFPTRKTAVINIFKTCISTEEWENTIQHIHPRGNKLTQTQLGKVEGDIIRFLNYGNDEKYAEDIIRCLNSGIEYKGTQINAWLQDYLKYRKGAKKIAGSLNYMLVKNQDDNRFKQLEIQQGVWGDSEEITLIASNLNVLSPFDQDDTDKYETYNV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9737	-	-

Protein sequence: 1002 a.a. .

                                    >EFF01806 NZ_CP012004:c2458526-2455521 Type VI Secretion System Effector (Tse) [Acinetobacter baumannii]
MNPYTIFEYTIVFYNQRNKELPNVKYSIIFFCEDGTKKTYEGATNPKGQTKPIPLNQNGKLHIFVEGHETIFSPKKTIKP
ILAEGSNIVEVNDIRLKSNTSFLTKAQYELMQKKTSKDLEELRKNAKAVKQKNFFNLSKIRPTFPLDLAEELNERINMSY
EEYRKKNTHIVKKTKYLKFKNYALYRFVDSAGNGIPIVDYQIFAQGQSRPLINARPGPPDKKGYTQLVYTHLKSRVTYTL
GSARKNSEWYEPITCVDKQTIYQIIFPTSIAVTQPDINHKENMEARQKPPIVINPTTNEVLILPSAVYAEFDKKTKILSD
AVREVHKSNANLIKAIQSRDLDEIKELEKRLNINQEKAIEKINGEFKQTADLREVWVVETTGKTNEKISKYNLKRRYLKV
TEYEELKAKRRNPQTEVDVTANQYTVQQPAQIRSSFEKLSQQLLTVKGNAGSDEKAVYNLIGGLGGEIAEEYKNSRDVTV
TQEAQWMRMVAGASGEGSISAAPKGVSIKTSGDMSAKWTLFEGVKEWRKFYPCETGWKLEYDNYDLGTIRFLIGAEISGF
SGANLGIAGNLSVDISHQGAAQVIKAVVRPPERSMSQMVDRNKKPMFQPAQGSLKIIGNNNQENAQNQGNISINAFAGVQ
IQGLLKGAVEWFKPKGDGSGEGEFVAIASAAAGGGVSAGVGAQGQFQIGYDQTSGNFKILVAAHLCWGMGAKGVASFVVG
TEHLLSYLGFIKSQVAHAGFKTLLYINEAAFLLAAQVLAYCIGENHPVISDINRIAASYGDWIRRLDIDQGRYKTAQNIN
SSSGKKELLYATPETKGILLYAVTHWTDRTAPIFDMNVKFSDMKIEFFPTRKTAVINIFKTCISTEEWENTIQHIHPRGN
KLTQTQLGKVEGDIIRFLNYGNDEKYAEDIIRCLNSGIEYKGTQINAWLQDYLKYRKGAKKIAGSLNYMLVKNQDDNRFK
QLEIQQGVWGDSEEITLIASNLNVLSPFDQDDTDKYETYNV*

                                

Nucleotide sequence: 3006 bp .

                                    >EFF01806 NZ_CP012004:c2458526-2455521 Type VI Secretion System Effector (Tse) [Acinetobacter baumannii]
ATGAATCCTTATACTATTTTTGAATATACAATAGTCTTTTATAATCAAAGAAATAAAGAATTACCAAATGTAAAATATAG
TATAATCTTTTTCTGTGAAGATGGAACAAAAAAGACTTATGAGGGTGCTACAAACCCAAAAGGACAAACAAAACCAATTC
CATTAAATCAAAATGGAAAATTGCATATTTTTGTTGAGGGGCATGAAACTATTTTCAGTCCTAAGAAGACTATAAAACCT
ATTTTGGCTGAAGGAAGCAATATAGTTGAAGTAAATGATATAAGACTTAAAAGCAATACTTCATTTCTAACAAAAGCTCA
ATATGAACTCATGCAGAAAAAAACAAGTAAAGATTTGGAAGAGTTAAGGAAAAATGCTAAAGCGGTTAAACAGAAAAATT
TTTTTAATTTATCGAAAATAAGACCAACTTTTCCTTTGGATTTGGCAGAAGAATTAAATGAAAGAATTAATATGTCTTAT
GAGGAATATAGAAAAAAGAATACTCATATCGTAAAAAAAACAAAATATCTTAAATTTAAAAACTATGCTTTATATAGATT
TGTCGATAGTGCTGGAAATGGTATTCCAATAGTGGATTATCAAATATTCGCTCAAGGCCAGAGTAGACCTTTAATAAATG
CAAGGCCGGGACCACCTGATAAAAAAGGATATACCCAATTGGTTTACACACACCTGAAAAGTAGAGTTACGTATACTTTA
GGATCTGCTAGAAAAAATAGTGAATGGTACGAGCCGATTACCTGTGTAGATAAACAAACCATTTATCAAATTATTTTTCC
GACTTCAATAGCAGTAACTCAGCCTGATATTAATCATAAAGAAAATATGGAGGCAAGACAAAAACCACCTATAGTGATTA
ACCCGACTACAAATGAAGTGCTTATTTTGCCGTCTGCAGTCTATGCAGAATTTGATAAAAAAACCAAGATATTATCTGAT
GCAGTGAGAGAAGTTCATAAGTCGAATGCAAATCTGATAAAGGCAATTCAAAGTCGTGATTTGGATGAAATCAAAGAATT
AGAAAAACGTTTAAATATAAATCAGGAAAAAGCCATTGAAAAAATTAATGGTGAATTTAAGCAAACAGCTGATCTAAGAG
AAGTGTGGGTGGTAGAGACCACAGGTAAAACAAATGAAAAAATATCTAAATATAATTTAAAACGCCGTTATTTAAAAGTT
ACGGAATATGAAGAGTTAAAAGCAAAACGTAGAAACCCTCAAACAGAAGTAGATGTAACTGCAAATCAATATACTGTACA
ACAGCCTGCACAAATTCGTAGTAGCTTTGAAAAACTTTCGCAGCAACTTTTGACAGTAAAAGGGAATGCCGGAAGTGATG
AGAAAGCTGTTTATAACCTGATAGGCGGTTTGGGTGGCGAAATTGCAGAAGAGTATAAAAATAGTAGGGATGTTACAGTT
ACTCAAGAAGCACAATGGATGCGTATGGTTGCTGGGGCAAGTGGTGAAGGTTCAATAAGTGCTGCACCAAAAGGAGTTAG
TATTAAGACTTCTGGAGATATGTCAGCTAAATGGACACTATTTGAAGGCGTTAAAGAATGGCGTAAATTTTATCCGTGCG
AAACAGGCTGGAAACTTGAATACGATAACTATGATTTAGGAACAATCCGGTTTTTAATTGGGGCGGAGATATCTGGTTTT
TCAGGAGCAAATTTAGGGATCGCTGGCAATCTATCTGTTGATATTTCTCATCAAGGAGCAGCTCAGGTTATAAAAGCTGT
AGTAAGACCGCCAGAAAGGTCAATGAGTCAAATGGTAGATCGTAACAAAAAACCTATGTTTCAGCCGGCACAAGGAAGTT
TAAAAATAATTGGTAATAATAATCAGGAAAATGCGCAGAATCAGGGTAATATCAGTATTAATGCTTTTGCTGGAGTTCAA
ATACAGGGGCTACTTAAAGGGGCTGTAGAGTGGTTTAAGCCTAAGGGGGATGGTAGTGGTGAAGGAGAATTTGTGGCGAT
TGCTTCAGCTGCAGCTGGCGGTGGTGTTAGTGCGGGCGTGGGGGCGCAAGGCCAGTTTCAAATAGGATATGATCAAACTT
CAGGTAATTTTAAAATATTGGTAGCGGCTCATTTGTGTTGGGGAATGGGAGCAAAAGGTGTGGCATCATTTGTTGTGGGT
ACGGAACATTTATTAAGTTATCTTGGTTTTATTAAATCCCAAGTTGCGCACGCAGGATTTAAAACGTTACTTTACATAAA
TGAAGCGGCTTTTTTGTTAGCTGCACAAGTTCTGGCTTATTGTATAGGTGAGAATCATCCAGTAATCAGTGATATAAATA
GAATTGCAGCTTCATATGGAGACTGGATTAGAAGGCTAGATATTGATCAAGGGCGCTATAAAACTGCACAGAATATTAAC
TCTAGTTCTGGAAAGAAGGAGTTATTATATGCTACACCAGAGACTAAAGGAATATTATTATATGCAGTAACTCATTGGAC
AGATAGAACTGCTCCTATTTTTGATATGAATGTTAAATTCTCAGATATGAAAATTGAATTTTTCCCAACTCGTAAGACAG
CAGTAATCAATATTTTTAAAACATGTATTTCTACAGAAGAGTGGGAAAATACGATTCAACATATTCATCCACGTGGTAAT
AAGCTTACTCAAACTCAATTAGGTAAAGTTGAGGGAGATATTATTCGTTTTCTTAATTATGGAAATGATGAAAAGTATGC
TGAAGATATAATCCGTTGTCTAAATTCTGGGATTGAATATAAAGGTACTCAAATCAATGCATGGCTTCAGGATTATTTAA
AATATCGTAAAGGAGCAAAAAAGATAGCAGGTTCTTTGAATTACATGTTGGTAAAAAATCAGGATGATAACCGATTTAAA
CAGCTAGAAATACAGCAGGGTGTATGGGGTGATTCAGAGGAAATAACACTAATAGCAAGTAATTTAAATGTTTTATCGCC
TTTTGATCAAGACGATACAGATAAATATGAAACATATAATGTGTAA

                                