SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01485
Effector type	Tde
Cognate immunity protein	IMU00306
Related T6SS (Type)	T6SS00312 (Type i4b)
Strain	Acinetobacter baumannii AB307-0294
Replicon	chromosome
Sequence	Protein sequence (474 a.a.); Nucleotide sequence (1422 bp)
Description	Rhs2 contained an AHH (alanine-histidine-histidine) motif within the C-terminal region that is characteristic of AHH superfamily nucleases.
Reference	[1] Fitzsimons TC, Lewis JM, Wright A, et al. Identification of Novel Acinetobacter baumannii Type VI Secretion System Antibacterial Effector and Immunity Pairs. Infect Immun. 2018 Jul 23;86(8). pii: e00297-18. doi: 10.1128/IAI.00297-18. Print 2018 Aug. PMID: 29735524

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ABBFA_RS11575
Coordinates (Strand)	2459651..2461072 (+)
NCBI ID	447105016
RefSeq	NZ_CP001172
Uniprot ID	A0A0J1A0A3_ACIBA, A0A0D5YJE0_ACIBA, SYC_ACIB5, SYC_ACIBY, SYC_ACIB3
KEGG ID	aby:ABAYE2493, abn:AB57_1408
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
tRNA-synt_1e	PF01406.21	1.7e-132	18..317
DALR_2	PF09190.13	1.3e-19	349..411

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-473	MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRANENGETIQQLTTRFIDAMNEDAANLGCLAPDEAPKATEYIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAGASERIDVEVEKKHPFDFVLWKHAKENEPSWASPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLMFPHHENEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTSLTRFYHSFKAYQQVYGQTTTEALDQSFIERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQDALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9922	-	-

Protein sequence: 474 a.a. .

                                    >EFF01485 NZ_CP001172:2459651-2461072 Type VI DNase and RNase Effector (Tde) [Acinetobacter baumannii AB307-0294]
MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRA
NENGETIQQLTTRFIDAMNEDAANLGCLAPDEAPKATEYIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGR
KLDDMQAGASERIDVEVEKKHPFDFVLWKHAKENEPSWASPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLMFPHHE
NEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTSL
TRFYHSFKAYQQVYGQTTTEALDQSFIERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNIL
GLVQHNVDDFLKSDIGQDALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD*

                                

Nucleotide sequence: 1422 bp .

                                    >EFF01485 NZ_CP001172:2459651-2461072 Type VI DNase and RNase Effector (Tde) [Acinetobacter baumannii AB307-0294]
ATGCAACCGTTTGTTTTATATAACTCTGAGCAACGAAAAAAAGTTGAATTTGTACCTCGCAAAGAAGGTCACATCGATAT
GTACGTCTGCGGTATGACCGTTTACGACTACTGTCATATCGGGCATGCTCGAGTTATGGTTGCATTTGACTACATTATCC
GTTTTTTGCGTAGTCAAGGCTGGAAAGTCCGCTACATTCGCAACATTACCGATATCGATGACAAAATCATCAAACGTGCG
AATGAGAATGGTGAAACAATCCAACAGCTCACCACGCGTTTCATCGATGCCATGAATGAAGATGCAGCGAACTTAGGCTG
TTTAGCACCAGATGAAGCACCTAAAGCGACTGAATATATTGACCAGATGCAAAATATGATTGGTAATCTGGTAAATAAAG
GCGCTGCTTACCCTGCTTCAAACGGTGATGTGTATTTTGAAGTCACCAAATTTGAAAAATATGGACGTCTCTCTGGCCGT
AAGCTTGATGACATGCAAGCTGGCGCAAGTGAGCGTATTGATGTAGAAGTTGAAAAGAAACATCCTTTTGACTTTGTGCT
TTGGAAACATGCAAAAGAAAATGAACCGTCTTGGGCATCCCCTTGGGGTAATGGTCGTCCGGGTTGGCACATTGAATGTT
CTGCAATGTCGACTTGCTGCCTAGGCAATCACTTTGACATTCATGGTGGCGGTTCAGATTTAATGTTCCCGCACCATGAA
AATGAAATTGCGCAAAGTGAAGCTTCGACTGGTGAGCAATATGTAAACTACTGGATGCATGTTGGCTTTATTAACGTTGA
TGGTGAAAAGATGTCTAAGTCTTTAGGCAACTTCTTTACGATTCGTGACGTAATGGAGAAATTCCACCCAGAAGTGATTC
GATACTTCATTGTGTCTTCACACTATCGTAGCCCTGTGAACTTCTCTGATGTAGCACTTAAAGAGGCAAAAACTTCTTTA
ACTCGTTTCTATCATTCATTTAAAGCTTACCAACAAGTGTACGGTCAAACGACAACTGAAGCGCTTGATCAAAGCTTTAT
TGAACGCTTTAACAATGCAATGTGTGACGATTTCAATACTGCCGAAGCAATGGCTGTATTGTTTGAACTGAACAAAGAGT
TAAACCGTGCTGTAAAAGAAGAGCAAGCTGACCAAGCGACTGTGCTTTATTCGACATTACGTCACCTCACCAACATTTTA
GGTTTGGTACAACACAATGTAGACGATTTCTTAAAATCAGATATTGGACAAGATGCACTTGCTTTGTCTGATGCTGAAAT
TGAAGATTTCATTCAACAACGTGTTGATGCGAAAAAAGCAAAAGACTTTGCTAAAGCAGATAGCATTCGTCAGTCTTTAC
TTGAGCAAGGCGTAGTTCTTGAAGACACTCGTCAAGGTACAGTTTGGCGTCGTGCTGATTAA

                                