SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01451
Effector type	Tse
Cognate immunity protein	IMU00311
Related T6SS (Type)	T6SS00926 (Type iii)
Strain	Bacteroides fragilis 638R
Replicon	chromosome
Sequence	Protein sequence (332 a.a.); Nucleotide sequence (996 bp)
Description	Bfe1 (putative transmembrane protein)
Reference	[1] Chatzidaki-Livanis M, Geva-Zatorsky N, Comstock LE. Bacteroides fragilis type VI secretion systems use novel effector and immunity proteins to antagonize human gut Bacteroidales species. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3627-32. doi: 10.1073/pnas.1522510113. Epub 2016 Mar 7. PMID: 26951680

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BF638R_1988
Coordinates (Strand)	2345779..2346774 (-)
NCBI ID	301162963
RefSeq	NC_016776
Uniprot ID	E1WVU5_BACF6
KEGG ID	bfg:BF638R_1988
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-278	MAYRNEYKTQSEFNVILERGDDYEGFVVGLGYTWMSSKVILPVNQNGWSPISRNVSVDESFHTIVSERKYDTSQYAYEKSLMQDPTKVSEKVRDLIVKNKGNNITEINLGQEKQYLPTDNSQISIVITDTGSRYEIVISATDNSNGKTYEAKYESLTDLVSAVRDSGSLPAVNKEGPNLEGLAGLGFGIAETAGNWAEKIMDNRGAYLPKQMRFSPKTLPPIIRLPLGNYQVPAKGMSRVRGVGKALGWAGMVLTGYQVVRDVQNGRFAVAGTRIAVA
Transmembrane helix	279-301	GLAYGVTFIPYVGWVLAIGIGVA
Outside	302-331	DYTWGDEFYDWIDNRASELEMWWDGVRLAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9938	-	-

Protein sequence: 332 a.a. .

                                    >EFF01451 NC_016776:c2346774-2345779 Type VI Secretion System Effector (Tse) [Bacteroides fragilis 638R]
MAYRNEYKTQSEFNVILERGDDYEGFVVGLGYTWMSSKVILPVNQNGWSPISRNVSVDESFHTIVSERKYDTSQYAYEKS
LMQDPTKVSEKVRDLIVKNKGNNITEINLGQEKQYLPTDNSQISIVITDTGSRYEIVISATDNSNGKTYEAKYESLTDLV
SAVRDSGSLPAVNKEGPNLEGLAGLGFGIAETAGNWAEKIMDNRGAYLPKQMRFSPKTLPPIIRLPLGNYQVPAKGMSRV
RGVGKALGWAGMVLTGYQVVRDVQNGRFAVAGTRIAVAGLAYGVTFIPYVGWVLAIGIGVADYTWGDEFYDWIDNRASEL
EMWWDGVRLAL*

                                

Nucleotide sequence: 996 bp .

                                    >EFF01451 NC_016776:c2346774-2345779 Type VI Secretion System Effector (Tse) [Bacteroides fragilis 638R]
ATGGCTTATAGAAATGAATATAAAACGCAAAGTGAATTTAATGTCATCTTGGAACGGGGGGATGACTATGAAGGATTTGT
AGTTGGCTTGGGATATACTTGGATGAGTAGCAAGGTAATTCTACCGGTAAATCAAAACGGATGGAGTCCAATATCTCGGA
ATGTTTCGGTGGACGAGAGTTTTCATACGATAGTCTCAGAAAGGAAATACGATACCTCCCAATATGCCTATGAAAAAAGT
CTGATGCAAGATCCAACAAAAGTTTCCGAAAAGGTCCGTGACTTAATAGTTAAAAACAAAGGTAATAATATCACTGAGAT
AAATTTAGGCCAAGAAAAGCAATATTTGCCCACGGATAATAGTCAGATAAGTATTGTAATTACTGACACTGGCAGTCGGT
ACGAAATTGTGATTAGTGCCACGGATAACTCAAATGGAAAGACTTATGAGGCAAAGTATGAGAGTTTGACGGACTTGGTG
TCAGCGGTACGCGATTCTGGTAGCCTGCCTGCTGTAAATAAGGAAGGACCCAATCTGGAAGGACTGGCAGGGTTAGGATT
CGGAATTGCTGAAACAGCCGGAAATTGGGCTGAGAAGATTATGGATAATCGAGGTGCGTACTTACCTAAGCAGATGCGTT
TCTCGCCCAAAACGCTTCCGCCGATTATAAGATTGCCTTTAGGGAACTATCAAGTCCCCGCTAAAGGTATGAGTAGAGTG
CGTGGAGTAGGTAAAGCTTTGGGATGGGCAGGAATGGTGCTTACTGGCTATCAAGTTGTGCGTGATGTGCAAAATGGTCG
ATTTGCCGTGGCAGGTACAAGAATTGCCGTAGCAGGTTTAGCTTATGGCGTTACTTTTATTCCCTATGTCGGTTGGGTCT
TAGCTATCGGTATCGGAGTGGCTGATTATACTTGGGGTGACGAGTTCTATGACTGGATAGACAATAGAGCTTCTGAATTG
GAAATGTGGTGGGACGGTGTAAGATTAGCATTATGA

                                