Detailed information of effector
Summary
| Effector ID | EFF00544  |
| Effector type | Tse |
| Cognate immunity protein | IMU00108 |
| Related T6SS (Type) | T6SS00443 (Type i3) |
| Strain | Methylomonas methanica MC09 |
| Replicon | chromosome |
| Sequence | Protein sequence (160 a.a.); Nucleotide sequence (480 bp) |
| Description | - |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | METME_RS17335 |
| Coordinates (Strand) | 3824035..3824514 (-) |
| NCBI ID | 333985114 |
| RefSeq | NC_015572 |
| Uniprot ID | UPI00020EB7F5 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ADPRTs_Tse2 | PF18648.3 | 2e-62 | 7..153 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-159 | MSYDYEKTSIDLYRAIFKGNFTTDVSCYLKPDKDLADADSIAPILHPTFSELSGEGALRRPPDIIANPDTGMYDPDTGGTSVFNRPRVLRRAEGDFFIPSGTDIPPDLKVKKDSYNERLKATHYTIMPLKPMFKESLMGQLDNFVRNAIRRQYEKARGL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9627 | - | - |
Protein sequence: 160 a.a. .
>EFF00544 NC_015572:c3824514-3824035 Type VI Secretion System Effector (Tse) [Methylomonas methanica MC09]
MSYDYEKTSIDLYRAIFKGNFTTDVSCYLKPDKDLADADSIAPILHPTFSELSGEGALRRPPDIIANPDTGMYDPDTGGT
SVFNRPRVLRRAEGDFFIPSGTDIPPDLKVKKDSYNERLKATHYTIMPLKPMFKESLMGQLDNFVRNAIRRQYEKARGL*
MSYDYEKTSIDLYRAIFKGNFTTDVSCYLKPDKDLADADSIAPILHPTFSELSGEGALRRPPDIIANPDTGMYDPDTGGT
SVFNRPRVLRRAEGDFFIPSGTDIPPDLKVKKDSYNERLKATHYTIMPLKPMFKESLMGQLDNFVRNAIRRQYEKARGL*
Nucleotide sequence: 480 bp .
>EFF00544 NC_015572:c3824514-3824035 Type VI Secretion System Effector (Tse) [Methylomonas methanica MC09]
ATGAGTTATGACTATGAAAAAACGAGTATTGACCTCTATCGCGCAATATTTAAGGGGAATTTCACAACGGATGTAAGTTG
TTATCTGAAACCGGACAAGGATTTAGCGGACGCAGACTCAATAGCACCAATTCTCCATCCAACTTTTAGCGAGCTATCAG
GAGAGGGGGCGCTAAGAAGGCCACCAGACATCATCGCTAACCCGGATACAGGAATGTATGATCCAGACACTGGAGGGACA
TCTGTGTTTAATCGACCTAGAGTATTGCGTCGTGCTGAAGGAGACTTTTTTATACCCTCAGGCACAGACATTCCACCTGA
TTTAAAAGTAAAGAAAGATAGTTACAACGAGCGCTTGAAGGCGACACACTATACAATCATGCCATTAAAACCAATGTTCA
AAGAAAGTCTCATGGGCCAGCTTGATAACTTCGTACGGAACGCTATTCGACGCCAATACGAAAAAGCTCGTGGATTGTAG
ATGAGTTATGACTATGAAAAAACGAGTATTGACCTCTATCGCGCAATATTTAAGGGGAATTTCACAACGGATGTAAGTTG
TTATCTGAAACCGGACAAGGATTTAGCGGACGCAGACTCAATAGCACCAATTCTCCATCCAACTTTTAGCGAGCTATCAG
GAGAGGGGGCGCTAAGAAGGCCACCAGACATCATCGCTAACCCGGATACAGGAATGTATGATCCAGACACTGGAGGGACA
TCTGTGTTTAATCGACCTAGAGTATTGCGTCGTGCTGAAGGAGACTTTTTTATACCCTCAGGCACAGACATTCCACCTGA
TTTAAAAGTAAAGAAAGATAGTTACAACGAGCGCTTGAAGGCGACACACTATACAATCATGCCATTAAAACCAATGTTCA
AAGAAAGTCTCATGGGCCAGCTTGATAACTTCGTACGGAACGCTATTCGACGCCAATACGAAAAAGCTCGTGGATTGTAG